Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles
The paper provides new information on β-glucans biosynthesis capacity and the morphological features modification of cells and colonies of Saccharomyces cerevisiae CNMN-Y-20 yeast strain known as producer of β-glucans. It was found that the development cycle and bioproductive capacity of the yeast w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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"Alexandru Ioan Cuza" University of Iași
2018-05-01
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| Series: | Journal of Experimental and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/10 |
| _version_ | 1797230665991716864 |
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| author | Agafia Uastîi Natalia Chiseliţa Nadejda Efremova Alina Beşliu |
| author_facet | Agafia Uastîi Natalia Chiseliţa Nadejda Efremova Alina Beşliu |
| author_sort | Agafia Uastîi |
| collection | DOAJ |
| description | The paper provides new information on β-glucans biosynthesis capacity and the morphological features modification of cells and colonies of Saccharomyces cerevisiae CNMN-Y-20 yeast strain known as producer of β-glucans. It was found that the development cycle and bioproductive capacity of the yeast was affected by cultivation in the presence of ZnO in a concentrations and the contact duration manner. Within 6-24 hours, the reproduction of the nanomodified cell was decreased compared to the control, but after 120 hours of submerged cultivation an insignificant increase in biomass content relative to 1L culture medium was recorded predominantly related to nanoparticle concentrations 0.5-1.0 mg/L. ZnO nanoparticles (<100 nm). Concentrations of 0.5-15 mg/L initiated a 13-15% increase in average dimensions of Saccharomyces cerevisiae CNMN-Y-20 cells and 7-12% increase in the ability to form of β-glucans, especially at concentrations of 1 and 5 mg/L. The correlation coefficient between the cells area and the β-glucans amount is strong (R² = 0.8021). The results provide the possibility of enhancing of the range of analyzes and the formation of reference bases necessary for the strategy to enlarge the biotechnological performance of yeasts. |
| first_indexed | 2024-04-24T15:32:06Z |
| format | Article |
| id | doaj.art-7ee30a5121b741fc9711ff9bd62d1e23 |
| institution | Directory Open Access Journal |
| issn | 2601-6974 |
| language | English |
| last_indexed | 2024-04-24T15:32:06Z |
| publishDate | 2018-05-01 |
| publisher | "Alexandru Ioan Cuza" University of Iași |
| record_format | Article |
| series | Journal of Experimental and Molecular Biology |
| spelling | doaj.art-7ee30a5121b741fc9711ff9bd62d1e232024-04-02T03:32:43Zeng"Alexandru Ioan Cuza" University of IașiJournal of Experimental and Molecular Biology2601-69742018-05-01191Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticlesAgafia UastîiNatalia ChiseliţaNadejda EfremovaAlina BeşliuThe paper provides new information on β-glucans biosynthesis capacity and the morphological features modification of cells and colonies of Saccharomyces cerevisiae CNMN-Y-20 yeast strain known as producer of β-glucans. It was found that the development cycle and bioproductive capacity of the yeast was affected by cultivation in the presence of ZnO in a concentrations and the contact duration manner. Within 6-24 hours, the reproduction of the nanomodified cell was decreased compared to the control, but after 120 hours of submerged cultivation an insignificant increase in biomass content relative to 1L culture medium was recorded predominantly related to nanoparticle concentrations 0.5-1.0 mg/L. ZnO nanoparticles (<100 nm). Concentrations of 0.5-15 mg/L initiated a 13-15% increase in average dimensions of Saccharomyces cerevisiae CNMN-Y-20 cells and 7-12% increase in the ability to form of β-glucans, especially at concentrations of 1 and 5 mg/L. The correlation coefficient between the cells area and the β-glucans amount is strong (R² = 0.8021). The results provide the possibility of enhancing of the range of analyzes and the formation of reference bases necessary for the strategy to enlarge the biotechnological performance of yeasts.http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/10ZnO nanoparticles,Saccharomyces cerevisiae,β-glucans,morphological features |
| spellingShingle | Agafia Uastîi Natalia Chiseliţa Nadejda Efremova Alina Beşliu Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles Journal of Experimental and Molecular Biology ZnO nanoparticles, Saccharomyces cerevisiae, β-glucans, morphological features |
| title | Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles |
| title_full | Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles |
| title_fullStr | Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles |
| title_full_unstemmed | Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles |
| title_short | Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles |
| title_sort | biosynthesis of β glucans and morphological features saccharomyces cerevisiae cnmn y 20 yeasts under the action of zinc oxide nanoparticles |
| topic | ZnO nanoparticles, Saccharomyces cerevisiae, β-glucans, morphological features |
| url | http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/10 |
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