MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer
Abstract Background Aberrant expression of microRNAs (miRNAs) contributes to the development of high grade serous ovarian cancer (HGSOC). However, the molecular mechanism by which miRNA-585-3p mediates high-grade serous ovarian carcinogenesis is unclear. This study aims to investigate the specific m...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-07-01
|
| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13048-021-00841-w |
| _version_ | 1797968490286546944 |
|---|---|
| author | Xiaoyuan Lu Guilin Li Sicong Liu Haihong Wang Buze Chen |
| author_facet | Xiaoyuan Lu Guilin Li Sicong Liu Haihong Wang Buze Chen |
| author_sort | Xiaoyuan Lu |
| collection | DOAJ |
| description | Abstract Background Aberrant expression of microRNAs (miRNAs) contributes to the development of high grade serous ovarian cancer (HGSOC). However, the molecular mechanism by which miRNA-585-3p mediates high-grade serous ovarian carcinogenesis is unclear. This study aims to investigate the specific mechanism of action of miR-585-3p in HGSOC. Methods Expression of miR-585-3p in HGSOC tissues and cell lines was detected by qRT-PCR. Cell viability and migration were detected using MTT and transwell system. The expression of target genes and target proteins of miR-585-3p was detected by dual luciferase reporter assay and western blot. Results The expression of miR-585-3p was significantly lower in HGSOC tissues and cells than in normal ovarian tissues and cell lines. In HGSOC tissues, CAPN9 expression was inversely correlated with miR-585-3p expression. MiR-585-3p inhibited the proliferation and migration of HGSOC cells. MiR-585-3p bound to the 3'-untranslated region (UTR) of CAPN9 and inhibits CAPN9 expression. Overexpression of CAPN9 reduced the inhibitory effect of miR-585-3p in HGSOC cells. Conclusions miR-585-3p is significantly down-regulated in HGSOC tissues and cell lines. MiR-585-3p inhibits the proliferation and migration of HGSOC cells by targeting CAPN9. |
| first_indexed | 2024-04-11T02:47:42Z |
| format | Article |
| id | doaj.art-7ee3804e82614387ac8838d96e31cd40 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:47:42Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-7ee3804e82614387ac8838d96e31cd402023-01-02T17:35:35ZengBMCJournal of Ovarian Research1757-22152021-07-011411710.1186/s13048-021-00841-wMiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancerXiaoyuan Lu0Guilin Li1Sicong Liu2Haihong Wang3Buze Chen4Department of Gynecology, The Affiliated Hospital of Xuzhou Medical UniversityDepartment of Gynecology, Maternal and Child Health Care Hospital Affiliated to Xuzhou Medical UniversityGraduate School, Xuzhou Medical UniversityDepartment of Gynecology, The Affiliated Hospital of Xuzhou Medical UniversityDepartment of Gynecology, The Affiliated Hospital of Xuzhou Medical UniversityAbstract Background Aberrant expression of microRNAs (miRNAs) contributes to the development of high grade serous ovarian cancer (HGSOC). However, the molecular mechanism by which miRNA-585-3p mediates high-grade serous ovarian carcinogenesis is unclear. This study aims to investigate the specific mechanism of action of miR-585-3p in HGSOC. Methods Expression of miR-585-3p in HGSOC tissues and cell lines was detected by qRT-PCR. Cell viability and migration were detected using MTT and transwell system. The expression of target genes and target proteins of miR-585-3p was detected by dual luciferase reporter assay and western blot. Results The expression of miR-585-3p was significantly lower in HGSOC tissues and cells than in normal ovarian tissues and cell lines. In HGSOC tissues, CAPN9 expression was inversely correlated with miR-585-3p expression. MiR-585-3p inhibited the proliferation and migration of HGSOC cells. MiR-585-3p bound to the 3'-untranslated region (UTR) of CAPN9 and inhibits CAPN9 expression. Overexpression of CAPN9 reduced the inhibitory effect of miR-585-3p in HGSOC cells. Conclusions miR-585-3p is significantly down-regulated in HGSOC tissues and cell lines. MiR-585-3p inhibits the proliferation and migration of HGSOC cells by targeting CAPN9.https://doi.org/10.1186/s13048-021-00841-wOvarian cancermiR-585-3pCAPN9BioinformaticsCell biology |
| spellingShingle | Xiaoyuan Lu Guilin Li Sicong Liu Haihong Wang Buze Chen MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer Journal of Ovarian Research Ovarian cancer miR-585-3p CAPN9 Bioinformatics Cell biology |
| title | MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer |
| title_full | MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer |
| title_fullStr | MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer |
| title_full_unstemmed | MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer |
| title_short | MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer |
| title_sort | mir 585 3p suppresses tumor proliferation and migration by directly targeting capn9 in high grade serous ovarian cancer |
| topic | Ovarian cancer miR-585-3p CAPN9 Bioinformatics Cell biology |
| url | https://doi.org/10.1186/s13048-021-00841-w |
| work_keys_str_mv | AT xiaoyuanlu mir5853psuppressestumorproliferationandmigrationbydirectlytargetingcapn9inhighgradeserousovariancancer AT guilinli mir5853psuppressestumorproliferationandmigrationbydirectlytargetingcapn9inhighgradeserousovariancancer AT sicongliu mir5853psuppressestumorproliferationandmigrationbydirectlytargetingcapn9inhighgradeserousovariancancer AT haihongwang mir5853psuppressestumorproliferationandmigrationbydirectlytargetingcapn9inhighgradeserousovariancancer AT buzechen mir5853psuppressestumorproliferationandmigrationbydirectlytargetingcapn9inhighgradeserousovariancancer |