Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication
Objectives: The clinical utility of sonication as an adjunctive diagnostic tool for the microbial diagnosis of cardiac implantable device-associated infections (CIDAIs) was investigated. Methods: The implants of 83 subjects were investigated, 15 with a CIDAI and 68 without a clinical infection. Clin...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S120197121500185X |
| _version_ | 1819171757304578048 |
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| author | Ricardo Cantarim Inacio Giselle Burlamaqui Klautau Maria Aparecida S. Murça Cely Barreto da Silva Stanley Nigro Luiz Antonio Rivetti Wilson Lopes Pereira Mauro Jose Costa Salles |
| author_facet | Ricardo Cantarim Inacio Giselle Burlamaqui Klautau Maria Aparecida S. Murça Cely Barreto da Silva Stanley Nigro Luiz Antonio Rivetti Wilson Lopes Pereira Mauro Jose Costa Salles |
| author_sort | Ricardo Cantarim Inacio |
| collection | DOAJ |
| description | Objectives: The clinical utility of sonication as an adjunctive diagnostic tool for the microbial diagnosis of cardiac implantable device-associated infections (CIDAIs) was investigated.
Methods: The implants of 83 subjects were investigated, 15 with a CIDAI and 68 without a clinical infection. Clinical data were analyzed prospectively and sonication fluid cultures (83 patients, 100%) and traditional cultures (31 patients, 37.4%) were performed
Results: Generator pocket infection and device-related endocarditis were found in 13 (86.7%) and four (26.7%) subjects, respectively. The mean numbers of previous technical complications and infections were higher in the infected patients compared to the non-infected patients (8 vs. 1, p < 0.001; 2 vs. 0, p < 0.031, respectively). The sensitivity and specificity for detecting CIDAI was 73.3% (11/15) and 48.5% (33/68) for sonication fluid culture, and 26.7% (4/15) and 100% (16/16) for traditional culture (p < 0.001), respectively. A higher number of organisms were identified by sonication fluid than by tissue culture (58 vs. 4 specimens; p < 0.001). The most frequent organisms cultured were Gram-positive cocci (66.1%), mainly coagulase-negative staphylococci (35.5%). Thirty-five (51.5%) non-infected subjects were considered colonized due to the positive identification of organisms exclusively through sonication fluid culture.
Conclusions: Sonication fluid culture from the removed cardiac implants has the potential to improve the microbiological diagnosis of CIDAIs. |
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| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-22T19:56:22Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-7ee4dc3bc65d46748a18d0bb9b3d27f32022-12-21T18:14:25ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112015-09-0138C545910.1016/j.ijid.2015.07.018Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonicationRicardo Cantarim Inacio0Giselle Burlamaqui Klautau1Maria Aparecida S. Murça2Cely Barreto da Silva3Stanley Nigro4Luiz Antonio Rivetti5Wilson Lopes Pereira6Mauro Jose Costa Salles7Emergency Service, Santa Casa de São Paulo School of Medical Sciences, São Paulo, BrazilDepartment of Internal Medicine, Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, Rua Dr Cesáreo Mota Jr 112, CEP 01303-060, São Paulo, SP, BrazilDepartment of Laboratory Medicine and Pathology, Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, BrazilDepartment of Laboratory Medicine and Pathology, Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, BrazilDepartment of Laboratory Medicine and Pathology, Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, BrazilDepartment of Surgery, Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, BrazilDepartment of Surgery, Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, São Paulo, BrazilDepartment of Internal Medicine, Division of Infectious Diseases, Santa Casa de São Paulo School of Medical Sciences, Rua Dr Cesáreo Mota Jr 112, CEP 01303-060, São Paulo, SP, BrazilObjectives: The clinical utility of sonication as an adjunctive diagnostic tool for the microbial diagnosis of cardiac implantable device-associated infections (CIDAIs) was investigated. Methods: The implants of 83 subjects were investigated, 15 with a CIDAI and 68 without a clinical infection. Clinical data were analyzed prospectively and sonication fluid cultures (83 patients, 100%) and traditional cultures (31 patients, 37.4%) were performed Results: Generator pocket infection and device-related endocarditis were found in 13 (86.7%) and four (26.7%) subjects, respectively. The mean numbers of previous technical complications and infections were higher in the infected patients compared to the non-infected patients (8 vs. 1, p < 0.001; 2 vs. 0, p < 0.031, respectively). The sensitivity and specificity for detecting CIDAI was 73.3% (11/15) and 48.5% (33/68) for sonication fluid culture, and 26.7% (4/15) and 100% (16/16) for traditional culture (p < 0.001), respectively. A higher number of organisms were identified by sonication fluid than by tissue culture (58 vs. 4 specimens; p < 0.001). The most frequent organisms cultured were Gram-positive cocci (66.1%), mainly coagulase-negative staphylococci (35.5%). Thirty-five (51.5%) non-infected subjects were considered colonized due to the positive identification of organisms exclusively through sonication fluid culture. Conclusions: Sonication fluid culture from the removed cardiac implants has the potential to improve the microbiological diagnosis of CIDAIs.http://www.sciencedirect.com/science/article/pii/S120197121500185XSonicationCardiac implantable electronic devicesBiofilmColonizationInfection |
| spellingShingle | Ricardo Cantarim Inacio Giselle Burlamaqui Klautau Maria Aparecida S. Murça Cely Barreto da Silva Stanley Nigro Luiz Antonio Rivetti Wilson Lopes Pereira Mauro Jose Costa Salles Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication International Journal of Infectious Diseases Sonication Cardiac implantable electronic devices Biofilm Colonization Infection |
| title | Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication |
| title_full | Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication |
| title_fullStr | Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication |
| title_full_unstemmed | Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication |
| title_short | Microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication |
| title_sort | microbial diagnosis of infection and colonization of cardiac implantable electronic devices by use of sonication |
| topic | Sonication Cardiac implantable electronic devices Biofilm Colonization Infection |
| url | http://www.sciencedirect.com/science/article/pii/S120197121500185X |
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