The effects of whole-body vibration on wound healing in a mouse pressure ulcer model
Background: Pressure ulcers are one of the most common complications of immobility resulting from pressure and shear. Whole-body vibration (WBV) has been shown to increase skin blood flow but little information is known about its effect on pressure ulcers. This study investigated the effects of WBV...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021009968 |
| _version_ | 1819138706408210432 |
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| author | Nattaya Wano Sompol Sanguanrungsirikul Somboon Keelawat Juraiporn Somboonwong |
| author_facet | Nattaya Wano Sompol Sanguanrungsirikul Somboon Keelawat Juraiporn Somboonwong |
| author_sort | Nattaya Wano |
| collection | DOAJ |
| description | Background: Pressure ulcers are one of the most common complications of immobility resulting from pressure and shear. Whole-body vibration (WBV) has been shown to increase skin blood flow but little information is known about its effect on pressure ulcers. This study investigated the effects of WBV on wound healing in a mouse pressure ulcer model. Methods: Two cycles of ischemia-reperfusion were performed by external application of two magnetic plates to dorsal skin to induce stage II pressure ulcers characterized by partial-thickness skin loss with exposed dermis. A total of 32 male ICR mice were randomly and equally divided into untreated control and the WBV groups. Immediately after the completion of 2-cycle ischemia-reperfusion injury, mice in the WBV group participated in a WBV program using a vibrator (frequency 45 Hz, peak acceleration 0.4 g, vertical motion) for 30 min/day and 5 consecutive days/week. At days 7 and 14 post-ulceration, wound closure rate was assessed. Wound tissues were harvested for determination of collagen deposition in Masson's trichrome stained sections, neutrophil infiltration and capillary density in hematoxylin and eosin-stained sections, as well as TNF-α and VEGF levels using ELISA. Results: TNF-α levels and neutrophil infiltration were significantly decreased in wounds on days 7 and 14 of WBV treatment. Moreover, wound closure rate and collagen deposition were remarkably accelerated on day 14. Tissue VEGF and capillary density were unaffected by WBV at either time point. Conclusions: These findings suggest that WBV has the potential to promote the healing process of stage II pressure ulcers, as evidenced by attenuation of wound inflammation and enhancement collagen deposition. |
| first_indexed | 2024-12-22T11:11:02Z |
| format | Article |
| id | doaj.art-7ee84238be7f473dbfdb3892b67dd461 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-22T11:11:02Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-7ee84238be7f473dbfdb3892b67dd4612022-12-21T18:28:09ZengElsevierHeliyon2405-84402021-04-0174e06893The effects of whole-body vibration on wound healing in a mouse pressure ulcer modelNattaya Wano0Sompol Sanguanrungsirikul1Somboon Keelawat2Juraiporn Somboonwong3Medical Science Program, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandDepartment of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandDepartment of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandDepartment of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Corresponding author.Background: Pressure ulcers are one of the most common complications of immobility resulting from pressure and shear. Whole-body vibration (WBV) has been shown to increase skin blood flow but little information is known about its effect on pressure ulcers. This study investigated the effects of WBV on wound healing in a mouse pressure ulcer model. Methods: Two cycles of ischemia-reperfusion were performed by external application of two magnetic plates to dorsal skin to induce stage II pressure ulcers characterized by partial-thickness skin loss with exposed dermis. A total of 32 male ICR mice were randomly and equally divided into untreated control and the WBV groups. Immediately after the completion of 2-cycle ischemia-reperfusion injury, mice in the WBV group participated in a WBV program using a vibrator (frequency 45 Hz, peak acceleration 0.4 g, vertical motion) for 30 min/day and 5 consecutive days/week. At days 7 and 14 post-ulceration, wound closure rate was assessed. Wound tissues were harvested for determination of collagen deposition in Masson's trichrome stained sections, neutrophil infiltration and capillary density in hematoxylin and eosin-stained sections, as well as TNF-α and VEGF levels using ELISA. Results: TNF-α levels and neutrophil infiltration were significantly decreased in wounds on days 7 and 14 of WBV treatment. Moreover, wound closure rate and collagen deposition were remarkably accelerated on day 14. Tissue VEGF and capillary density were unaffected by WBV at either time point. Conclusions: These findings suggest that WBV has the potential to promote the healing process of stage II pressure ulcers, as evidenced by attenuation of wound inflammation and enhancement collagen deposition.http://www.sciencedirect.com/science/article/pii/S2405844021009968Whole-body vibrationPressure ulcerIschemia-reperfusion injuryVascular endothelial growth factor (VEGF)Tumor necrosis factor alpha (TNF-α)Wound healing |
| spellingShingle | Nattaya Wano Sompol Sanguanrungsirikul Somboon Keelawat Juraiporn Somboonwong The effects of whole-body vibration on wound healing in a mouse pressure ulcer model Heliyon Whole-body vibration Pressure ulcer Ischemia-reperfusion injury Vascular endothelial growth factor (VEGF) Tumor necrosis factor alpha (TNF-α) Wound healing |
| title | The effects of whole-body vibration on wound healing in a mouse pressure ulcer model |
| title_full | The effects of whole-body vibration on wound healing in a mouse pressure ulcer model |
| title_fullStr | The effects of whole-body vibration on wound healing in a mouse pressure ulcer model |
| title_full_unstemmed | The effects of whole-body vibration on wound healing in a mouse pressure ulcer model |
| title_short | The effects of whole-body vibration on wound healing in a mouse pressure ulcer model |
| title_sort | effects of whole body vibration on wound healing in a mouse pressure ulcer model |
| topic | Whole-body vibration Pressure ulcer Ischemia-reperfusion injury Vascular endothelial growth factor (VEGF) Tumor necrosis factor alpha (TNF-α) Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021009968 |
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