A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint
Phytohormone auxin controls various aspects of plant growth and development. The typical auxin signalling involves the degradation of canonical Aux/IAA proteins upon auxin perception releasing the auxin response factors (ARF) to activate auxin-regulated gene expression. Extensive research has been p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1284125/full |
| _version_ | 1797324343180525568 |
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| author | Vaishnavi Amarr Reddy Jolly Madathiparambil Saju Kumar Nadimuthu Rajani Sarojam |
| author_facet | Vaishnavi Amarr Reddy Jolly Madathiparambil Saju Kumar Nadimuthu Rajani Sarojam |
| author_sort | Vaishnavi Amarr Reddy |
| collection | DOAJ |
| description | Phytohormone auxin controls various aspects of plant growth and development. The typical auxin signalling involves the degradation of canonical Aux/IAA proteins upon auxin perception releasing the auxin response factors (ARF) to activate auxin-regulated gene expression. Extensive research has been pursued in deciphering the role of canonical Aux/IAAs, however, the function of non-canonical Aux/IAA genes remains elusive. Here we identified a non-canonical Aux/IAA gene, MsIAA32 from spearmint (Mentha spicata), which lacks the TIR1-binding domain and shows its involvement in the development of peltate glandular trichomes (PGT), which are the sites for production and storage of commercially important essential oils. Using yeast two-hybrid studies, two canonical Aux/IAAs, MsIAA3, MsIAA4 and an ARF, MsARF3 were identified as the preferred binding partners of MsIAA32. Expression of a R2R3-MYB gene MsMYB36 and a cyclin gene MsCycB2-4 was altered in MsIAA32 suppressed plants indicating that these genes are possible downstream targets of MsIAA32 mediated signalling. Ectopic expression of MsIAA32 in Arabidopsis affected non-glandular trichome formation along with other auxin related developmental traits. Our findings establish the role of non-canonical Aux/IAA mediated auxin signalling in PGT development and reveal species-specific functionalization of Aux/IAAs. |
| first_indexed | 2024-03-08T05:54:46Z |
| format | Article |
| id | doaj.art-7eea2384a36b4a18be1852021b42340d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T05:54:46Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7eea2384a36b4a18be1852021b42340d2024-02-05T04:39:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.12841251284125A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmintVaishnavi Amarr ReddyJolly Madathiparambil SajuKumar NadimuthuRajani SarojamPhytohormone auxin controls various aspects of plant growth and development. The typical auxin signalling involves the degradation of canonical Aux/IAA proteins upon auxin perception releasing the auxin response factors (ARF) to activate auxin-regulated gene expression. Extensive research has been pursued in deciphering the role of canonical Aux/IAAs, however, the function of non-canonical Aux/IAA genes remains elusive. Here we identified a non-canonical Aux/IAA gene, MsIAA32 from spearmint (Mentha spicata), which lacks the TIR1-binding domain and shows its involvement in the development of peltate glandular trichomes (PGT), which are the sites for production and storage of commercially important essential oils. Using yeast two-hybrid studies, two canonical Aux/IAAs, MsIAA3, MsIAA4 and an ARF, MsARF3 were identified as the preferred binding partners of MsIAA32. Expression of a R2R3-MYB gene MsMYB36 and a cyclin gene MsCycB2-4 was altered in MsIAA32 suppressed plants indicating that these genes are possible downstream targets of MsIAA32 mediated signalling. Ectopic expression of MsIAA32 in Arabidopsis affected non-glandular trichome formation along with other auxin related developmental traits. Our findings establish the role of non-canonical Aux/IAA mediated auxin signalling in PGT development and reveal species-specific functionalization of Aux/IAAs.https://www.frontiersin.org/articles/10.3389/fpls.2024.1284125/fullRNAipeltate glandular trichomesAux/IAAARFauxinspearmint |
| spellingShingle | Vaishnavi Amarr Reddy Jolly Madathiparambil Saju Kumar Nadimuthu Rajani Sarojam A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint Frontiers in Plant Science RNAi peltate glandular trichomes Aux/IAA ARF auxin spearmint |
| title | A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint |
| title_full | A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint |
| title_fullStr | A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint |
| title_full_unstemmed | A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint |
| title_short | A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint |
| title_sort | non canonical aux iaa gene msiaa32 regulates peltate glandular trichome development in spearmint |
| topic | RNAi peltate glandular trichomes Aux/IAA ARF auxin spearmint |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1284125/full |
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