Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall Interactions
The number of cases of failure in the treatment of infections associated with resistant bacteria is on the rise, due to the decreasing efficacy of current antibiotics. Notably, 7α-Acetoxy-6β-hydroxyroyleanone (AHR), a diterpene isolated from different <i>Plectranthus</i> species, showed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/7/983 |
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| author | Filipe Pereira Teresa Figueiredo Rodrigo F. M. de Almeida Catarina A. C. Antunes Catarina Garcia Catarina P. Reis Lia Ascensão Rita G. Sobral Patricia Rijo |
| author_facet | Filipe Pereira Teresa Figueiredo Rodrigo F. M. de Almeida Catarina A. C. Antunes Catarina Garcia Catarina P. Reis Lia Ascensão Rita G. Sobral Patricia Rijo |
| author_sort | Filipe Pereira |
| collection | DOAJ |
| description | The number of cases of failure in the treatment of infections associated with resistant bacteria is on the rise, due to the decreasing efficacy of current antibiotics. Notably, 7α-Acetoxy-6β-hydroxyroyleanone (AHR), a diterpene isolated from different <i>Plectranthus</i> species, showed antibacterial activity, namely against Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains. The high antibacterial activity and low cytotoxicity render this natural compound an interesting alternative against resistant bacteria. The aim of this study is to understand the mechanism of action of AHR on MRSA, using the MRSA/Vancomycin-intermediate <i>S. aureus</i> (VISA) strain CIP 106760, and to study the AHR effect on lipid bilayers and on the cell wall. Although AHR interacted with lipid bilayers, it did not have a significant effect on membrane passive permeability. Alternatively, bacteria treated with this royleanone displayed cell wall disruption, without revealing cell lysis. In conclusion, the results gathered so far point to a yet undescribed mode of action that needs further investigation. |
| first_indexed | 2024-03-10T18:46:32Z |
| format | Article |
| id | doaj.art-7eeee9dd63e346b1b0c1a4246b096ee6 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T18:46:32Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-7eeee9dd63e346b1b0c1a4246b096ee62023-11-20T05:29:30ZengMDPI AGBiomolecules2218-273X2020-06-0110798310.3390/biom10070983Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall InteractionsFilipe Pereira0Teresa Figueiredo1Rodrigo F. M. de Almeida2Catarina A. C. Antunes3Catarina Garcia4Catarina P. Reis5Lia Ascensão6Rita G. Sobral7Patricia Rijo8Research Center for Biosciences and Health Technologies (CBIOS), Universidade Lusófona de Humanidades e Tecnologias, Campo Grande, 376, 1749-024 Lisboa, PortugalDepartamento de Ciências da Vida, UCIBIO@REQUIMTE, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2825-149 Caparica, PortugalCentro de Química Estrutural, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, PortugalCentro de Química Estrutural, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, PortugalResearch Center for Biosciences and Health Technologies (CBIOS), Universidade Lusófona de Humanidades e Tecnologias, Campo Grande, 376, 1749-024 Lisboa, PortugalInstituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003 Lisboa, PortugalCentro de Estudos do Ambiente e do Mar (CESAM), Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, PortugalDepartamento de Ciências da Vida, UCIBIO@REQUIMTE, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2825-149 Caparica, PortugalResearch Center for Biosciences and Health Technologies (CBIOS), Universidade Lusófona de Humanidades e Tecnologias, Campo Grande, 376, 1749-024 Lisboa, PortugalThe number of cases of failure in the treatment of infections associated with resistant bacteria is on the rise, due to the decreasing efficacy of current antibiotics. Notably, 7α-Acetoxy-6β-hydroxyroyleanone (AHR), a diterpene isolated from different <i>Plectranthus</i> species, showed antibacterial activity, namely against Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains. The high antibacterial activity and low cytotoxicity render this natural compound an interesting alternative against resistant bacteria. The aim of this study is to understand the mechanism of action of AHR on MRSA, using the MRSA/Vancomycin-intermediate <i>S. aureus</i> (VISA) strain CIP 106760, and to study the AHR effect on lipid bilayers and on the cell wall. Although AHR interacted with lipid bilayers, it did not have a significant effect on membrane passive permeability. Alternatively, bacteria treated with this royleanone displayed cell wall disruption, without revealing cell lysis. In conclusion, the results gathered so far point to a yet undescribed mode of action that needs further investigation.https://www.mdpi.com/2218-273X/10/7/9837α-Acetoxy-6β-hydroxyroyleanoneMRSAantibacterial activitymembrane interactioncell wall |
| spellingShingle | Filipe Pereira Teresa Figueiredo Rodrigo F. M. de Almeida Catarina A. C. Antunes Catarina Garcia Catarina P. Reis Lia Ascensão Rita G. Sobral Patricia Rijo Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall Interactions Biomolecules 7α-Acetoxy-6β-hydroxyroyleanone MRSA antibacterial activity membrane interaction cell wall |
| title | Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall Interactions |
| title_full | Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall Interactions |
| title_fullStr | Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall Interactions |
| title_full_unstemmed | Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall Interactions |
| title_short | Unveiling the Mechanism of Action of 7α-acetoxy-6β-hydroxyroyleanone on an MRSA/VISA Strain: Membrane and Cell Wall Interactions |
| title_sort | unveiling the mechanism of action of 7α acetoxy 6β hydroxyroyleanone on an mrsa visa strain membrane and cell wall interactions |
| topic | 7α-Acetoxy-6β-hydroxyroyleanone MRSA antibacterial activity membrane interaction cell wall |
| url | https://www.mdpi.com/2218-273X/10/7/983 |
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