Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton
Glutamate decarboxylase (GAD) mainly regulated the biosynthesis of γ-aminobutyric acid (GABA) and played an important role in plant growth and stress resistance. To explore the potential function of GAD in cotton growth, the genome-wide identification, structure, and expression analysis of GAD genes...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.965058/full |
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| author | Hui Huang Yunxin He Aihua Cui Liangqing Sun Mingge Han Jing Wang Cun Rui Yuqian Lei Xiaoyu Liu Nan Xu Hong Zhang Yuexin Zhang Yapeng Fan Xixian Feng Kesong Ni Jie Jiang Xingping Zhang Chao Chen Shuai Wang Xiugui Chen Xuke Lu Delong Wang Junjuan Wang Zujun Yin Bobokhonova Zebinisso Qaraevna Lixue Guo Lanjie Zhao Wuwei Ye |
| author_facet | Hui Huang Yunxin He Aihua Cui Liangqing Sun Mingge Han Jing Wang Cun Rui Yuqian Lei Xiaoyu Liu Nan Xu Hong Zhang Yuexin Zhang Yapeng Fan Xixian Feng Kesong Ni Jie Jiang Xingping Zhang Chao Chen Shuai Wang Xiugui Chen Xuke Lu Delong Wang Junjuan Wang Zujun Yin Bobokhonova Zebinisso Qaraevna Lixue Guo Lanjie Zhao Wuwei Ye |
| author_sort | Hui Huang |
| collection | DOAJ |
| description | Glutamate decarboxylase (GAD) mainly regulated the biosynthesis of γ-aminobutyric acid (GABA) and played an important role in plant growth and stress resistance. To explore the potential function of GAD in cotton growth, the genome-wide identification, structure, and expression analysis of GAD genes were performed in this study. There were 10, 9, 5, and 5 GAD genes identified in G. hirsutum, G. barbadense, G. arboreum, and G. raimondii, respectively. GAD was divided into four clades according to the protein motif composition, gene structure, and phylogenetic relationship. The segmental duplication was the main way of the GAD gene family evolution. Most GhGADs respond to abiotic stress. Clade Ⅲ GAD was induced by Cd2+ stress, especially GhGAD6, and silencing GhGAD6 would lead to more serious Cd2+ poisoning in cotton. The oxidative damage caused by Cd2+ stress was relieved by increasing the GABA content. It was speculated that the decreased expression of GhGAD6 reduced the content of GABA in vivo and caused the accumulation of ROS. This study will further expand our understanding of the relationship between the evolution and function of the GhGAD gene family and provide new genetic resources for cotton breeding under environmental stress and phytoremediation. |
| first_indexed | 2024-04-12T05:11:42Z |
| format | Article |
| id | doaj.art-7eef985325114e269627cfdff8910a5d |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-12T05:11:42Z |
| publishDate | 2022-09-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-7eef985325114e269627cfdff8910a5d2022-12-22T03:46:45ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-09-011310.3389/fgene.2022.965058965058Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cottonHui Huang0Yunxin He1Aihua Cui2Liangqing Sun3Mingge Han4Jing Wang5Cun Rui6Yuqian Lei7Xiaoyu Liu8Nan Xu9Hong Zhang10Yuexin Zhang11Yapeng Fan12Xixian Feng13Kesong Ni14Jie Jiang15Xingping Zhang16Chao Chen17Shuai Wang18Xiugui Chen19Xuke Lu20Delong Wang21Junjuan Wang22Zujun Yin23Bobokhonova Zebinisso Qaraevna24Lixue Guo25Lanjie Zhao26Wuwei Ye27Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaHunan Institute of Cotton Science, Changde, ChinaCotton Research Institute of Jiangxi Province, Jiujiang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaHunan Institute of Cotton Science, Changde, ChinaHunan Institute of Cotton Science, Changde, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaDepartment Cotton Growing, Genetics, Breeding and Seed, Tajik Agrarian University Named Shirinsho Shotemur Dushanbe, Dushanbe, TajikistanInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaInstitute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, ChinaGlutamate decarboxylase (GAD) mainly regulated the biosynthesis of γ-aminobutyric acid (GABA) and played an important role in plant growth and stress resistance. To explore the potential function of GAD in cotton growth, the genome-wide identification, structure, and expression analysis of GAD genes were performed in this study. There were 10, 9, 5, and 5 GAD genes identified in G. hirsutum, G. barbadense, G. arboreum, and G. raimondii, respectively. GAD was divided into four clades according to the protein motif composition, gene structure, and phylogenetic relationship. The segmental duplication was the main way of the GAD gene family evolution. Most GhGADs respond to abiotic stress. Clade Ⅲ GAD was induced by Cd2+ stress, especially GhGAD6, and silencing GhGAD6 would lead to more serious Cd2+ poisoning in cotton. The oxidative damage caused by Cd2+ stress was relieved by increasing the GABA content. It was speculated that the decreased expression of GhGAD6 reduced the content of GABA in vivo and caused the accumulation of ROS. This study will further expand our understanding of the relationship between the evolution and function of the GhGAD gene family and provide new genetic resources for cotton breeding under environmental stress and phytoremediation.https://www.frontiersin.org/articles/10.3389/fgene.2022.965058/fullGhGAD6Cd2+GABAexpressionevolutionary |
| spellingShingle | Hui Huang Yunxin He Aihua Cui Liangqing Sun Mingge Han Jing Wang Cun Rui Yuqian Lei Xiaoyu Liu Nan Xu Hong Zhang Yuexin Zhang Yapeng Fan Xixian Feng Kesong Ni Jie Jiang Xingping Zhang Chao Chen Shuai Wang Xiugui Chen Xuke Lu Delong Wang Junjuan Wang Zujun Yin Bobokhonova Zebinisso Qaraevna Lixue Guo Lanjie Zhao Wuwei Ye Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton Frontiers in Genetics GhGAD6 Cd2+ GABA expression evolutionary |
| title | Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton |
| title_full | Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton |
| title_fullStr | Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton |
| title_full_unstemmed | Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton |
| title_short | Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton |
| title_sort | genome wide identification of gad family genes suggests ghgad6 functionally respond to cd2 stress in cotton |
| topic | GhGAD6 Cd2+ GABA expression evolutionary |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.965058/full |
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