Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory Animals
Extraction was by cold maceration using methanol. The extract was concentrated in vacuo to yield a brown solid of 120.191 g. The crude methanol extract was partitioned into n-hexane 0.1 g, dichloromethane 2.5 g, ethylacetate 4.0 g, and methanol 10.0 g fractions via coarse chromatography. Secondary m...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Altezoro s.r.o. (Slovak Republic) and Publishing Center "Dialog" (Ukraine)
2022-07-01
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| Series: | Traektoriâ Nauki |
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| Online Access: | https://pathofscience.org/index.php/ps/article/view/1324 |
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| author | Odo Peter Odo Ekene Ihemadu Chiaguguom Felix Grace Nwaubani Daniel Amako Ngozi Omekara Israel |
| author_facet | Odo Peter Odo Ekene Ihemadu Chiaguguom Felix Grace Nwaubani Daniel Amako Ngozi Omekara Israel |
| author_sort | Odo Peter |
| collection | DOAJ |
| description | Extraction was by cold maceration using methanol. The extract was concentrated in vacuo to yield a brown solid of 120.191 g. The crude methanol extract was partitioned into n-hexane 0.1 g, dichloromethane 2.5 g, ethylacetate 4.0 g, and methanol 10.0 g fractions via coarse chromatography. Secondary metabolites identified; 21.73±0.36% alkaloids, 40.78±0.27% flavonoids, 15.99±0.044% saponins, 6.088±0.06% tannins, 3.086±0.03% terpenoids and 12.13±0.01% Cardiac Glycosides. Acute toxicity test showed no death in the rats administered with low dose (1000 mg/kg) and high dose (6000 mg/kg). Analgesic activities of crude methanol extract and fractions showed that at a low dose of 4000 mg/kg of the extract and the fractions have percentage inhibition of pains as methanol crude (63.48±4.62), methanol fraction (79.14±7.39), dichloromethane fraction (60.79±6.69), ethylacetate fraction (23.26±9.75) and n-hexane fraction (64.82±9.75). At a high dose of 8000 mg/kg, the percentage inhibition of pain was 5200±2.00, 71.87±7.04, 80.31±6.20, 45.61±12.60 and 43.87±8.13. Statistical analysis; P>0.05 confidence level, methanol fraction recorded highest analgesic activities while ethylacetate fraction had least. |
| first_indexed | 2024-04-11T10:14:04Z |
| format | Article |
| id | doaj.art-7ef05908d96f412cb3fb9d95c95a2a31 |
| institution | Directory Open Access Journal |
| issn | 2413-9009 |
| language | English |
| last_indexed | 2024-04-11T10:14:04Z |
| publishDate | 2022-07-01 |
| publisher | Altezoro s.r.o. (Slovak Republic) and Publishing Center "Dialog" (Ukraine) |
| record_format | Article |
| series | Traektoriâ Nauki |
| spelling | doaj.art-7ef05908d96f412cb3fb9d95c95a2a312022-12-22T04:30:01ZengAltezoro s.r.o. (Slovak Republic) and Publishing Center "Dialog" (Ukraine)Traektoriâ Nauki2413-90092022-07-01873001301010.22178/pos.83-2808Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory AnimalsOdo Peter0Odo Ekene1Ihemadu Chiaguguom2Felix Grace3Nwaubani Daniel4Amako Ngozi5Omekara Israel6Michael Okpara University of Agriculture, UmudikeMichael Okpara University of Agriculture, UmudikeMichael Okpara University of Agriculture, UmudikeMichael Okpara University of Agriculture, UmudikeMichael Okpara University of Agriculture, UmudikeMichael Okpara University of Agriculture, UmudikeMichael Okpara University of Agriculture, UmudikeExtraction was by cold maceration using methanol. The extract was concentrated in vacuo to yield a brown solid of 120.191 g. The crude methanol extract was partitioned into n-hexane 0.1 g, dichloromethane 2.5 g, ethylacetate 4.0 g, and methanol 10.0 g fractions via coarse chromatography. Secondary metabolites identified; 21.73±0.36% alkaloids, 40.78±0.27% flavonoids, 15.99±0.044% saponins, 6.088±0.06% tannins, 3.086±0.03% terpenoids and 12.13±0.01% Cardiac Glycosides. Acute toxicity test showed no death in the rats administered with low dose (1000 mg/kg) and high dose (6000 mg/kg). Analgesic activities of crude methanol extract and fractions showed that at a low dose of 4000 mg/kg of the extract and the fractions have percentage inhibition of pains as methanol crude (63.48±4.62), methanol fraction (79.14±7.39), dichloromethane fraction (60.79±6.69), ethylacetate fraction (23.26±9.75) and n-hexane fraction (64.82±9.75). At a high dose of 8000 mg/kg, the percentage inhibition of pain was 5200±2.00, 71.87±7.04, 80.31±6.20, 45.61±12.60 and 43.87±8.13. Statistical analysis; P>0.05 confidence level, methanol fraction recorded highest analgesic activities while ethylacetate fraction had least.https://pathofscience.org/index.php/ps/article/view/1324alkaloidscardiac glycosidesflavonoidspainsrats |
| spellingShingle | Odo Peter Odo Ekene Ihemadu Chiaguguom Felix Grace Nwaubani Daniel Amako Ngozi Omekara Israel Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory Animals Traektoriâ Nauki alkaloids cardiac glycosides flavonoids pains rats |
| title | Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory Animals |
| title_full | Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory Animals |
| title_fullStr | Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory Animals |
| title_full_unstemmed | Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory Animals |
| title_short | Phytochemical Screening, Acute Toxicity and Analgesic Activity of Extracts From Newbouldia laevis in Laboratory Animals |
| title_sort | phytochemical screening acute toxicity and analgesic activity of extracts from newbouldia laevis in laboratory animals |
| topic | alkaloids cardiac glycosides flavonoids pains rats |
| url | https://pathofscience.org/index.php/ps/article/view/1324 |
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