Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling Pathway
Cosmetic ingredients originating from natural resources have garnered considerable attention, and the demand for whitening ingredients is increasing, particularly in Asian countries. Lignin is a natural phenolic biopolymer significantly effective as a natural sunscreen, as its ultraviolet protection...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/13/7462 |
| _version_ | 1797479525891702784 |
|---|---|
| author | Moon-Hee Choi Seung-Hwa Yang Won-Keun Park Hyun-Jae Shin |
| author_facet | Moon-Hee Choi Seung-Hwa Yang Won-Keun Park Hyun-Jae Shin |
| author_sort | Moon-Hee Choi |
| collection | DOAJ |
| description | Cosmetic ingredients originating from natural resources have garnered considerable attention, and the demand for whitening ingredients is increasing, particularly in Asian countries. Lignin is a natural phenolic biopolymer significantly effective as a natural sunscreen, as its ultraviolet protection efficacy ranges from 250 to 400 nm. However, using different types of lignin as cosmetic ingredients is difficult owing to the heterogeneity of lignin and the lack of in vitro and in vivo safety and efficacy data. Thus, steam-exploded lignin (SEL) was prepared from bamboo, fractionated via successive organic solvent extraction, and sequentially fractionated using ethyl acetate, methanol, and acetone to investigate its potential as a natural whitening material. Gel permeation chromatography showed that the molecular weight of acetone-soluble and acetone-insoluble SEL fractions were the lowest and the highest, respectively. Monomer structures of the four lignin fractions were elucidated using <sup>1</sup>H, <sup>13</sup>C, and 2D heteronuclear single quantum coherence nuclear magnetic resonance and pyrolysis gas chromatography/mass spectrometry. The antioxidant and tyrosinase inhibition activities of the four fractions were compared. The methanol-soluble SEL fraction (SEL-F2) showed the highest antioxidant activity (except 2,2-diphenyl-1-picrylhydrazyl scavenging activity), and the enzyme inhibition kinetics were confirmed. In this study, the expression pattern of the anti-melanogenic-related proteins by SEL-F2 was confirmed for the first time via the protein kinase A (PKA)/cAMP-response element-binding (CREB) protein signaling pathway in B16F10 melanoma cells. Thus, SEL may serve as a valuable cosmetic whitening ingredient. |
| first_indexed | 2024-03-09T21:46:46Z |
| format | Article |
| id | doaj.art-7ef1be686908405caf756731ffc95193 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:46:46Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7ef1be686908405caf756731ffc951932023-11-23T20:14:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012313746210.3390/ijms23137462Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling PathwayMoon-Hee Choi0Seung-Hwa Yang1Won-Keun Park2Hyun-Jae Shin3Department of Beauty and Cosmetics, Graduate School of Industrial Technology Startup, Chosun University, 309 Pilmundaero, Gwangju 61452, KoreaDepartment of Chemical Engineering, Graduate School of Chosun University, Gwangju 61452, KoreaDepartment of Chemical Energy Engineering, Sangmyong University, Seoul 03016, KoreaDepartment of Beauty and Cosmetics, Graduate School of Industrial Technology Startup, Chosun University, 309 Pilmundaero, Gwangju 61452, KoreaCosmetic ingredients originating from natural resources have garnered considerable attention, and the demand for whitening ingredients is increasing, particularly in Asian countries. Lignin is a natural phenolic biopolymer significantly effective as a natural sunscreen, as its ultraviolet protection efficacy ranges from 250 to 400 nm. However, using different types of lignin as cosmetic ingredients is difficult owing to the heterogeneity of lignin and the lack of in vitro and in vivo safety and efficacy data. Thus, steam-exploded lignin (SEL) was prepared from bamboo, fractionated via successive organic solvent extraction, and sequentially fractionated using ethyl acetate, methanol, and acetone to investigate its potential as a natural whitening material. Gel permeation chromatography showed that the molecular weight of acetone-soluble and acetone-insoluble SEL fractions were the lowest and the highest, respectively. Monomer structures of the four lignin fractions were elucidated using <sup>1</sup>H, <sup>13</sup>C, and 2D heteronuclear single quantum coherence nuclear magnetic resonance and pyrolysis gas chromatography/mass spectrometry. The antioxidant and tyrosinase inhibition activities of the four fractions were compared. The methanol-soluble SEL fraction (SEL-F2) showed the highest antioxidant activity (except 2,2-diphenyl-1-picrylhydrazyl scavenging activity), and the enzyme inhibition kinetics were confirmed. In this study, the expression pattern of the anti-melanogenic-related proteins by SEL-F2 was confirmed for the first time via the protein kinase A (PKA)/cAMP-response element-binding (CREB) protein signaling pathway in B16F10 melanoma cells. Thus, SEL may serve as a valuable cosmetic whitening ingredient.https://www.mdpi.com/1422-0067/23/13/7462antioxidant activitybamboo stemlignin fractionationPKA/CREB pathwaysteam explosiontyrosinase inhibition |
| spellingShingle | Moon-Hee Choi Seung-Hwa Yang Won-Keun Park Hyun-Jae Shin Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling Pathway International Journal of Molecular Sciences antioxidant activity bamboo stem lignin fractionation PKA/CREB pathway steam explosion tyrosinase inhibition |
| title | Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling Pathway |
| title_full | Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling Pathway |
| title_fullStr | Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling Pathway |
| title_full_unstemmed | Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling Pathway |
| title_short | Bamboo Lignin Fractions with In Vitro Tyrosinase Inhibition Activity Downregulate Melanogenesis in B16F10 Cells via PKA/CREB Signaling Pathway |
| title_sort | bamboo lignin fractions with in vitro tyrosinase inhibition activity downregulate melanogenesis in b16f10 cells via pka creb signaling pathway |
| topic | antioxidant activity bamboo stem lignin fractionation PKA/CREB pathway steam explosion tyrosinase inhibition |
| url | https://www.mdpi.com/1422-0067/23/13/7462 |
| work_keys_str_mv | AT moonheechoi bambooligninfractionswithinvitrotyrosinaseinhibitionactivitydownregulatemelanogenesisinb16f10cellsviapkacrebsignalingpathway AT seunghwayang bambooligninfractionswithinvitrotyrosinaseinhibitionactivitydownregulatemelanogenesisinb16f10cellsviapkacrebsignalingpathway AT wonkeunpark bambooligninfractionswithinvitrotyrosinaseinhibitionactivitydownregulatemelanogenesisinb16f10cellsviapkacrebsignalingpathway AT hyunjaeshin bambooligninfractionswithinvitrotyrosinaseinhibitionactivitydownregulatemelanogenesisinb16f10cellsviapkacrebsignalingpathway |