One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no ‘two-in-one’ KI strategy combining...

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Bibliographic Details
Main Authors: Wenyuan Li, Yage Zhang, Bingzhou Han, Lianyan Li, Muhang Li, Xiaochan Lu, Cheng Chen, Mengjia Lu, Yujie Zhang, Xuefeng Jia, Zuoyan Zhu, Xiangjun Tong, Bo Zhang
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-10-01
Series:eLife
Subjects:
CRISPR/cas
NHEJ
targeted insertion
knockin
conditional knock out
gene-labeling
Online Access:https://elifesciences.org/articles/48081

Internet

https://elifesciences.org/articles/48081

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