Comparative study of sperms motility analysis with CASA by using leja and microscope slide
The aim of this study was to compare the assessment of spermatozoa motility by using leja and microscope slide (MS). Fresh semen of four Ongole crossbred (PO) bulls were collected by using an artificial vagina. The semen with 70% sperms motility was diluted into into 30 million/ml concentration and...
| প্রধান লেখক: | , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
| প্রকাশিত: |
Fakultas Peternakan Universitas Brawijaya
2020-08-01
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| মালা: | Jurnal Ilmu-Ilmu Peternakan |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://jiip.ub.ac.id/index.php/jiip/article/view/708 |
| _version_ | 1827942168710348800 |
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| author | Dian Ratnawati Muchamad Luthfi |
| author_facet | Dian Ratnawati Muchamad Luthfi |
| author_sort | Dian Ratnawati |
| collection | DOAJ |
| description | The aim of this study was to compare the assessment of spermatozoa motility by using leja and microscope slide (MS). Fresh semen of four Ongole crossbred (PO) bulls were collected by using an artificial vagina. The semen with 70% sperms motility was diluted into into 30 million/ml concentration and assessed for its motility by using CASA (SCA 5.2 Microptic). The measured parameters in this study were sperms motility, progressive sperm motility, curve linear velocity (VCL), straight linear velocity (VSL), average pathway velocity (VAP), linearity (LIN), straightness (STR), wobble (WOB), hyperactivity (H), lateral head amplitude (ALH) and beat cross frequency (BCF). The data were analyzed by using independent simple t-test (IBM SPSS statistics 24). The results showed a hyperactive spermatozoa when analyzed by using leja was at 7.3%, higher than assessed with Microscope slide (2.8%). Meanwhile, other motility parameters (motility, progressive motility, VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) did not show any difference. Microscope slides can be used as a means of supporting sperms motility assesment by using CASA and more efficient. |
| first_indexed | 2024-03-13T09:54:40Z |
| format | Article |
| id | doaj.art-7efddd1f9b6a466da4af07fa77fa72ad |
| institution | Directory Open Access Journal |
| issn | 0852-3681 2443-0765 |
| language | English |
| last_indexed | 2024-03-13T09:54:40Z |
| publishDate | 2020-08-01 |
| publisher | Fakultas Peternakan Universitas Brawijaya |
| record_format | Article |
| series | Jurnal Ilmu-Ilmu Peternakan |
| spelling | doaj.art-7efddd1f9b6a466da4af07fa77fa72ad2023-05-24T03:58:50ZengFakultas Peternakan Universitas BrawijayaJurnal Ilmu-Ilmu Peternakan0852-36812443-07652020-08-0130211512210.21776/ub.jiip.2020.030.02.03512Comparative study of sperms motility analysis with CASA by using leja and microscope slideDian Ratnawati0Muchamad Luthfi1Loka Penelitian Sapi Potong, Grati Pasuruan, IndonesiaLoka Penelitian Sapi PotongThe aim of this study was to compare the assessment of spermatozoa motility by using leja and microscope slide (MS). Fresh semen of four Ongole crossbred (PO) bulls were collected by using an artificial vagina. The semen with 70% sperms motility was diluted into into 30 million/ml concentration and assessed for its motility by using CASA (SCA 5.2 Microptic). The measured parameters in this study were sperms motility, progressive sperm motility, curve linear velocity (VCL), straight linear velocity (VSL), average pathway velocity (VAP), linearity (LIN), straightness (STR), wobble (WOB), hyperactivity (H), lateral head amplitude (ALH) and beat cross frequency (BCF). The data were analyzed by using independent simple t-test (IBM SPSS statistics 24). The results showed a hyperactive spermatozoa when analyzed by using leja was at 7.3%, higher than assessed with Microscope slide (2.8%). Meanwhile, other motility parameters (motility, progressive motility, VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) did not show any difference. Microscope slides can be used as a means of supporting sperms motility assesment by using CASA and more efficient.https://jiip.ub.ac.id/index.php/jiip/article/view/708ledjamicroscope slidesperms motilitycasa |
| spellingShingle | Dian Ratnawati Muchamad Luthfi Comparative study of sperms motility analysis with CASA by using leja and microscope slide Jurnal Ilmu-Ilmu Peternakan ledja microscope slide sperms motility casa |
| title | Comparative study of sperms motility analysis with CASA by using leja and microscope slide |
| title_full | Comparative study of sperms motility analysis with CASA by using leja and microscope slide |
| title_fullStr | Comparative study of sperms motility analysis with CASA by using leja and microscope slide |
| title_full_unstemmed | Comparative study of sperms motility analysis with CASA by using leja and microscope slide |
| title_short | Comparative study of sperms motility analysis with CASA by using leja and microscope slide |
| title_sort | comparative study of sperms motility analysis with casa by using leja and microscope slide |
| topic | ledja microscope slide sperms motility casa |
| url | https://jiip.ub.ac.id/index.php/jiip/article/view/708 |
| work_keys_str_mv | AT dianratnawati comparativestudyofspermsmotilityanalysiswithcasabyusinglejaandmicroscopeslide AT muchamadluthfi comparativestudyofspermsmotilityanalysiswithcasabyusinglejaandmicroscopeslide |