The effective dose and pattern of soybean extract administration to regulate body weight of laboratory rats
<p class="Pa5"><em>Abstrak </em><em> </em></p> <p class="Pa6"><strong><em>Latar Belakang: </em></strong><em>Protein dalam kedelai, β conglycinin mempunyai efek menekan nafsu makan melalui stimulasi Kolesistok...
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| Format: | Article |
| Language: | English |
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Badan Penelitian dan Pengembangan Kesehatan
2016-07-01
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| Series: | Health Science Journal of Indonesia |
| Subjects: | |
| Online Access: | http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/4916 |
| Summary: | <p class="Pa5"><em>Abstrak </em><em> </em></p> <p class="Pa6"><strong><em>Latar Belakang: </em></strong><em>Protein dalam kedelai, β conglycinin mempunyai efek menekan nafsu makan melalui stimulasi Kolesistokinin. Kolesistokinin adalah hormon yang dilepaskan di saluran pencernaan dipicu oleh asupan protein dan berefek menekan nafsu makan untuk jangka pendek. Detam 1 adalah jenis kedelai berkualitas tinggi berdasarkan dengan Menteri Pertanian Indonesia. Ekstrak protein kedelai Detam 1 oleh Deak metode mengandung kadar β conglycinin yang tinggi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak protein kedelai Detam 1 metode Deak dalam menurunkan asupan makanan, berat badan, dan kadar CCK plasma selama 14 dan 28 hari pada berbagai dosis dan pola pemberian pada tikus Wistar jantan. </em></p> <p class="Pa6"><strong><em>Metode: </em></strong><em>Terdapat sebelas kelompok perlakuan (n=3), kelompok dosis (5mg/1x//hari, 10mg/1x/hari, 20mg/1x/hari), (2,5mg/2x/hari, 5mg/2x/hari, 10mg/2x/hari) dan (1,7mg/3x/hari, 3,4mg/3x/ hari, 6,7mg/3x/ hari), kelompok kontrol negatif (akuades) dan kelompok positif kontrol (Sibutramine). asupan makanan (g), berat badan (g) dan pengukuran tingkat Cholecystokinin plasma dengan metode ELISA (ng / ml). </em></p> <p class="Pa6"><strong><em>Hasil: </em></strong><em>Hasil penelitian menunjukkan bahwa persentase penurunan asupan makanan yang terbaik adalah: kelompok 3,4mg/3x/hari (p <0,05), penghambatan berat badan selama 14 hari: kelompok 10 mg/1x/hari, selama 28 hari: kelompok 1,7mg/3x/hari (p <0,05), peningkatan kadar Kolesistokinin plasma: kelompok 20 mg / 1 x / hari (p <0,05). </em></p> <p class="Pa6"><strong><em>Simpulan: </em></strong><em>Dosis dan pola pemberian terbaik untuk menghambat berat badan selama 14 hari adalah ekstrak 10 mg sekali sehari di pagi hari, selama 28 hari adalah 1,7 mg tiga kali sehari. (<strong>Health Science Journal of Indonesia 2016;7:17-26) </strong></em></p> <p class="Pa6"><strong><em>Kata kunci: </em></strong><em>Kedelai Detam 1 -dosis efektif - berat badan - kolesistokinin </em></p> <p class="Pa5"><strong>Abstract</strong></p> <p class="Pa6"><strong>Background</strong>: Protein in soybean, β conglycinin is responsible for anti-obesity effects by suppressing appetite via stimulation of Cholecystokinin. Cholecystokinin is a hormone released in the digestive tract in response to the intake of protein and suppress appetite for short-term. <em>Detam </em>1 variety is a high-quality soybean according to the Minister of Agriculture of Indonesia. Soybean protein extract Detam 1 by Deak method contains high levels of β conglycinin. The purpose of this study was to determine the effective dose of protein extract Detam 1 soybean Deak Method (PEDSDM) in reducing food intake, regulate body weight, and plasma CCK level for 14 and 28 days at various dosage and pattern of treatment on male Wistar rats.</p> <p class="Pa6"><strong>Methods</strong>: There were eleven groups of treatment (n = 3), administrated with extracts at 5 mg/1x/day, 10 mg/1x/day, 20 mg/1x/day, 2.5 mg/2x/day, 5 mg/2x/day, 10 mg/2x/day and 1.7mg/3x/day, 3.4mg/3x/day, 6.7 mg/3x/day, negative control group (distilled water) and positive control group (Sibutramine). Food intake (g), weight loss (g) and measurement of plasma Cholecystokinin levels by ELISA (ng /ml)</p> <p class="Pa6"><strong>Results</strong>: The results showed that the highest percentage decrease in food intake is: group 3.4mg /3x/ day (p <0.05), inhibition weight gain for 14 days: group 10 mg /1x/ day, for 28 days: group 1.7 mg/3x/day (p <0.05), increased plasma Cholecystokinin levels: group 20 mg /1x/day (p <0.05).</p> <p class="Pa6"><strong>Conclusions: </strong>The effective dose and pattern administrating the rats for 14 days is extract of 10 mg once a day in the morning, for 28 days is 1.7 mg three times a day. <em>(<strong>Health Science Journal of Indonesia 2016;7:17-26) </strong></em></p> <strong>Keywords: </strong>Soybean var Detam 1 -effective dose - body weight - Cholecystokinin<p> </p> |
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| ISSN: | 2087-7021 2338-3437 |