THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTION
Aim: to determine the association between plasma concentrations of biomarkers (sCD40L, PDGF-BB, PlGF-1, ST2) with histochemical and immunohistochemical signs of heart rejection.Materials and methods. The study included 98 heart recipients aged from 12 to 69 (mean age 43 ± 14) years, of which 78 men....
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2017-01-01
|
| Series: | Vestnik Transplantologii i Iskusstvennyh Organov |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/702 |
| _version_ | 1797872007174422528 |
|---|---|
| author | O. P. Shevchenko A. A. Ulybysheva A. V. Aksyonova N. P. Mozhejko E. A. Stakhanova V. V. Dontsov E. A. Nikitina A. O. Shevchenko |
| author_facet | O. P. Shevchenko A. A. Ulybysheva A. V. Aksyonova N. P. Mozhejko E. A. Stakhanova V. V. Dontsov E. A. Nikitina A. O. Shevchenko |
| author_sort | O. P. Shevchenko |
| collection | DOAJ |
| description | Aim: to determine the association between plasma concentrations of biomarkers (sCD40L, PDGF-BB, PlGF-1, ST2) with histochemical and immunohistochemical signs of heart rejection.Materials and methods. The study included 98 heart recipients aged from 12 to 69 (mean age 43 ± 14) years, of which 78 men. In 68 patients dilated cardiomyopathy was diagnosed, 30 recipients were diagnosed with coronary heart disease. The concentrations of placental growth factor (PlGF-1), platelet-derived growth factor (PDGF-BB), soluble CD40 ligand (sCD40L) were measured using xMAP technology. The concentrations of ST2 cardiac biomarker were measured by ELISA.Results. No correlation was found between the levels of biomarkers (sCD40L, PDGF-BB, PlGF-1, ST2) and gender, age and diagnosis. The rejection was diagnosed via biopsy in 49 biopsies taken from 37 recipients. 1A rejection was found in 25 patients (34 biopsies), 1B rejection was identifi ed in 2 patients (3 biopsies), 3A rejection was diagnosed in 4 patients. Immunohistochemical signs of humoral rejection were identifi ed in 3 patients. The combination of acute cellular and humoral rejection was found in 4 patients (5 biopsies). The PDGFBB level was measured at the same day as the biopsy was taken, and it was shown to be signifi cantly higher in patients with rejection (p = 0.02). Rejection frequency was signifi cantly higher in patients with high PDGF-BB level (≥2473.7 pg/ml, RR = 1.64 ± 0.23; 95% CI [1.03–2.61]). Rejection frequency increased to 2.11 ± 0.34 [95% CI [1.08–4.11]] in recipients with ST2 and PDGF-BB concentration higher than the median value. The highest predictive value for heart rejection can be reached by a panel of three biomarkers: sCD40L, PlGF-1 and ST2 (RR = 2.51 ± 0.38; 95% CI [1.18–5.3]).Conclusion. PDGF-BB has moderate predictive value for heart rejection. The highest predictive value for heart rejection was reached by a panel of three biomarkers: sCD40L, PlGF-1 and ST2. |
| first_indexed | 2024-04-10T00:53:05Z |
| format | Article |
| id | doaj.art-7f00f4ead1bc4429b46a060583fd6a51 |
| institution | Directory Open Access Journal |
| issn | 1995-1191 |
| language | Russian |
| last_indexed | 2024-04-10T00:53:05Z |
| publishDate | 2017-01-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Vestnik Transplantologii i Iskusstvennyh Organov |
| spelling | doaj.art-7f00f4ead1bc4429b46a060583fd6a512023-03-13T10:37:23ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovVestnik Transplantologii i Iskusstvennyh Organov1995-11912017-01-01184717610.15825/1995-1191-2016-4-71-76586THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTIONO. P. Shevchenko0A. A. Ulybysheva1A. V. Aksyonova2N. P. Mozhejko3E. A. Stakhanova4V. V. Dontsov5E. A. Nikitina6A. O. Shevchenko7V.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation I.M. Sechenov First Moscow State Medical UniversityV.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation N.I. Pirogov Russian National Research Medical UniversityV.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian FederationI.M. Sechenov First Moscow State Medical UniversityV.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian FederationV.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation I.M. Sechenov First Moscow State Medical UniversityAim: to determine the association between plasma concentrations of biomarkers (sCD40L, PDGF-BB, PlGF-1, ST2) with histochemical and immunohistochemical signs of heart rejection.Materials and methods. The study included 98 heart recipients aged from 12 to 69 (mean age 43 ± 14) years, of which 78 men. In 68 patients dilated cardiomyopathy was diagnosed, 30 recipients were diagnosed with coronary heart disease. The concentrations of placental growth factor (PlGF-1), platelet-derived growth factor (PDGF-BB), soluble CD40 ligand (sCD40L) were measured using xMAP technology. The concentrations of ST2 cardiac biomarker were measured by ELISA.Results. No correlation was found between the levels of biomarkers (sCD40L, PDGF-BB, PlGF-1, ST2) and gender, age and diagnosis. The rejection was diagnosed via biopsy in 49 biopsies taken from 37 recipients. 1A rejection was found in 25 patients (34 biopsies), 1B rejection was identifi ed in 2 patients (3 biopsies), 3A rejection was diagnosed in 4 patients. Immunohistochemical signs of humoral rejection were identifi ed in 3 patients. The combination of acute cellular and humoral rejection was found in 4 patients (5 biopsies). The PDGFBB level was measured at the same day as the biopsy was taken, and it was shown to be signifi cantly higher in patients with rejection (p = 0.02). Rejection frequency was signifi cantly higher in patients with high PDGF-BB level (≥2473.7 pg/ml, RR = 1.64 ± 0.23; 95% CI [1.03–2.61]). Rejection frequency increased to 2.11 ± 0.34 [95% CI [1.08–4.11]] in recipients with ST2 and PDGF-BB concentration higher than the median value. The highest predictive value for heart rejection can be reached by a panel of three biomarkers: sCD40L, PlGF-1 and ST2 (RR = 2.51 ± 0.38; 95% CI [1.18–5.3]).Conclusion. PDGF-BB has moderate predictive value for heart rejection. The highest predictive value for heart rejection was reached by a panel of three biomarkers: sCD40L, PlGF-1 and ST2.https://journal.transpl.ru/vtio/article/view/702heart transplantationrejectionbiomarkersmultiplex analysisscd40lpdgf-bbplgf-1st2. |
| spellingShingle | O. P. Shevchenko A. A. Ulybysheva A. V. Aksyonova N. P. Mozhejko E. A. Stakhanova V. V. Dontsov E. A. Nikitina A. O. Shevchenko THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTION Vestnik Transplantologii i Iskusstvennyh Organov heart transplantation rejection biomarkers multiplex analysis scd40l pdgf-bb plgf-1 st2. |
| title | THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTION |
| title_full | THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTION |
| title_fullStr | THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTION |
| title_full_unstemmed | THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTION |
| title_short | THE DIAGNOSTIC VALUE OF PLATELET-DERIVED FACTOR PDGF-BB AND ST2 IN HEART REJECTION |
| title_sort | diagnostic value of platelet derived factor pdgf bb and st2 in heart rejection |
| topic | heart transplantation rejection biomarkers multiplex analysis scd40l pdgf-bb plgf-1 st2. |
| url | https://journal.transpl.ru/vtio/article/view/702 |
| work_keys_str_mv | AT opshevchenko thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT aaulybysheva thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT avaksyonova thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT npmozhejko thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT eastakhanova thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT vvdontsov thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT eanikitina thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT aoshevchenko thediagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT opshevchenko diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT aaulybysheva diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT avaksyonova diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT npmozhejko diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT eastakhanova diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT vvdontsov diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT eanikitina diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection AT aoshevchenko diagnosticvalueofplateletderivedfactorpdgfbbandst2inheartrejection |