RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cells
<p>Abstract</p> <p>Background</p> <p>A key factor underlying the control of the cellular growth, size and proliferation involves the regulation of the total protein synthesis. Most often, the initial stages of mRNA translation are rate limiting, which involves a group o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-06-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | http://www.wjso.com/content/10/1/119 |
| _version_ | 1828394028870139904 |
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| author | Wang Zheng Chen Jinxian Sun Jianhua Cui Zhe Wu Hui |
| author_facet | Wang Zheng Chen Jinxian Sun Jianhua Cui Zhe Wu Hui |
| author_sort | Wang Zheng |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>A key factor underlying the control of the cellular growth, size and proliferation involves the regulation of the total protein synthesis. Most often, the initial stages of mRNA translation are rate limiting, which involves a group of eukaryotic translation initiation factors (<it>EIFs</it>). Research advances focused on the inhibition of their expression and activity hold the key to the initiation and progression of tumor and tumor prognosis.</p> <p>Method</p> <p>We performed RNA interference (RNAi) with the lentivirus vector system to silence the <it>EIF3B</it> gene using the colon cancer cell strain SW1116. The negative control included the normal target cells infected with the negative control virus whereas the knockdown cells included the normal target cells transfected with the RNAi target virus. We tested the inhibition resulting from the decreased expression of <it>EIF3B</it> gene on the proliferation rate of SW1116 cells, including the cell cycle, apoptosis and clonability.</p> <p>Results</p> <p>Compared with the negative control, the impact of <it>EIF3B</it> gene expression in SW1116 cells on the levels of mRNA and protein in the knockdown group, was significantly inhibited (<it>P</it> <0.01). Furthermore, the cell proliferation rate and clonability were also significantly inhibited (<it>P</it> <0.01). The apoptosis rate increased significantly (<it>P</it> <0.05). A significant decrease in the number of cells in the G1 phase (<it>P</it> <0.01) and significant increases in S (<it>P</it> <0.01) and G2 phases (<it>P</it> <0.05) were observed.</p> <p>Conclusions</p> <p>The silencing of <it>EIF3B</it> gene expression inhibits the proliferation of colon cancer cells.</p> |
| first_indexed | 2024-12-10T07:48:26Z |
| format | Article |
| id | doaj.art-7f02dfc8c1e74384891506280bc037e1 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-10T07:48:26Z |
| publishDate | 2012-06-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-7f02dfc8c1e74384891506280bc037e12022-12-22T01:57:06ZengBMCWorld Journal of Surgical Oncology1477-78192012-06-0110111910.1186/1477-7819-10-119RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cellsWang ZhengChen JinxianSun JianhuaCui ZheWu Hui<p>Abstract</p> <p>Background</p> <p>A key factor underlying the control of the cellular growth, size and proliferation involves the regulation of the total protein synthesis. Most often, the initial stages of mRNA translation are rate limiting, which involves a group of eukaryotic translation initiation factors (<it>EIFs</it>). Research advances focused on the inhibition of their expression and activity hold the key to the initiation and progression of tumor and tumor prognosis.</p> <p>Method</p> <p>We performed RNA interference (RNAi) with the lentivirus vector system to silence the <it>EIF3B</it> gene using the colon cancer cell strain SW1116. The negative control included the normal target cells infected with the negative control virus whereas the knockdown cells included the normal target cells transfected with the RNAi target virus. We tested the inhibition resulting from the decreased expression of <it>EIF3B</it> gene on the proliferation rate of SW1116 cells, including the cell cycle, apoptosis and clonability.</p> <p>Results</p> <p>Compared with the negative control, the impact of <it>EIF3B</it> gene expression in SW1116 cells on the levels of mRNA and protein in the knockdown group, was significantly inhibited (<it>P</it> <0.01). Furthermore, the cell proliferation rate and clonability were also significantly inhibited (<it>P</it> <0.01). The apoptosis rate increased significantly (<it>P</it> <0.05). A significant decrease in the number of cells in the G1 phase (<it>P</it> <0.01) and significant increases in S (<it>P</it> <0.01) and G2 phases (<it>P</it> <0.05) were observed.</p> <p>Conclusions</p> <p>The silencing of <it>EIF3B</it> gene expression inhibits the proliferation of colon cancer cells.</p>http://www.wjso.com/content/10/1/119Eukaryotic initiation factorColon cancer cell SW1116Proliferation |
| spellingShingle | Wang Zheng Chen Jinxian Sun Jianhua Cui Zhe Wu Hui RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cells World Journal of Surgical Oncology Eukaryotic initiation factor Colon cancer cell SW1116 Proliferation |
| title | RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cells |
| title_full | RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cells |
| title_fullStr | RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cells |
| title_full_unstemmed | RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cells |
| title_short | RNA interference-mediated silencing of eukaryotic translation initiation factor 3, subunit B (<it>EIF3B</it>) gene expression inhibits proliferation of colon cancer cells |
| title_sort | rna interference mediated silencing of eukaryotic translation initiation factor 3 subunit b it eif3b it gene expression inhibits proliferation of colon cancer cells |
| topic | Eukaryotic initiation factor Colon cancer cell SW1116 Proliferation |
| url | http://www.wjso.com/content/10/1/119 |
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