Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri
In this study, 41 isolates of the genus Trichoderma including six species of Trichoderma arundinaceum, T. asperellum, T. atroviride, T. harzianum, T. longibrachiatum and T. virens were isolated from soils of cucumber fields in Kermanshah province, and their efficacy to control Phytophthora drechsler...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2020-02-01
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| Series: | Journal of Crop Protection |
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| Online Access: | http://jcp.modares.ac.ir/article-3-41406-en.html |
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| author | Marzieh Soheiliara Mahyar Sheikholeslami Hamid Reza Zamanizadeh |
| author_facet | Marzieh Soheiliara Mahyar Sheikholeslami Hamid Reza Zamanizadeh |
| author_sort | Marzieh Soheiliara |
| collection | DOAJ |
| description | In this study, 41 isolates of the genus Trichoderma including six species of Trichoderma arundinaceum, T. asperellum, T. atroviride, T. harzianum, T. longibrachiatum and T. virens were isolated from soils of cucumber fields in Kermanshah province, and their efficacy to control Phytophthora drechsleri, the causal agent of cucumber damping off, was investigated in laboratory and greenhouse conditions. In direct confrontation between Trichoderma isolates and the pathogen, all isolates were able to promote, establish, and sporulate on pathogen mycelia. Through dual culture test, the most inhibitory effect on pathogen growth (62.89%) was recorded for T. harzianum (isolate T1). Volatile compounds of T. harzianum (isolate T7) had the greatest mycelial growth inhibition (46.59%) compared with control. Complete growth inhibition of the pathogen was recorded when the growth medium was supplemented with culture filtrate of Trichoderma isolates at concentration of 15% and 30%. At the concentrations of 15% and 30%, 18 isolates and 22 isolates completely inhibited the pathogen expansion respectively. In the greenhouse, the results of data analysis on the survival percent of plants in various treatments showed significant differences between the isolates of Trichoderma in terms of preventing cucumber damping off. In conclusion, T. asperellum showed the highest ability to control Phytophthora damping off and promoting cucumber growth which is a new record for Iran. This species can be an appropriate choice for biological control of the disease caused by P. drechsleri in cucumber. |
| first_indexed | 2024-04-12T15:08:40Z |
| format | Article |
| id | doaj.art-7f0306c562314ba8b79b4eeb108b0c8e |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-04-12T15:08:40Z |
| publishDate | 2020-02-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-7f0306c562314ba8b79b4eeb108b0c8e2022-12-22T03:27:51ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2020-02-0192261274Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleriMarzieh Soheiliara0Mahyar Sheikholeslami1Hamid Reza Zamanizadeh2 College of Agriculture and Natural Resources, Islamic Azad University Science and Research Branch, Tehran, Iran. Plant Protection Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Kermanshah, Iran. College of Agriculture and Natural Resources, Islamic Azad University Science and Research Branch, Tehran, Iran. In this study, 41 isolates of the genus Trichoderma including six species of Trichoderma arundinaceum, T. asperellum, T. atroviride, T. harzianum, T. longibrachiatum and T. virens were isolated from soils of cucumber fields in Kermanshah province, and their efficacy to control Phytophthora drechsleri, the causal agent of cucumber damping off, was investigated in laboratory and greenhouse conditions. In direct confrontation between Trichoderma isolates and the pathogen, all isolates were able to promote, establish, and sporulate on pathogen mycelia. Through dual culture test, the most inhibitory effect on pathogen growth (62.89%) was recorded for T. harzianum (isolate T1). Volatile compounds of T. harzianum (isolate T7) had the greatest mycelial growth inhibition (46.59%) compared with control. Complete growth inhibition of the pathogen was recorded when the growth medium was supplemented with culture filtrate of Trichoderma isolates at concentration of 15% and 30%. At the concentrations of 15% and 30%, 18 isolates and 22 isolates completely inhibited the pathogen expansion respectively. In the greenhouse, the results of data analysis on the survival percent of plants in various treatments showed significant differences between the isolates of Trichoderma in terms of preventing cucumber damping off. In conclusion, T. asperellum showed the highest ability to control Phytophthora damping off and promoting cucumber growth which is a new record for Iran. This species can be an appropriate choice for biological control of the disease caused by P. drechsleri in cucumber.http://jcp.modares.ac.ir/article-3-41406-en.htmlantagonistbiological controlcucurbitspathogen |
| spellingShingle | Marzieh Soheiliara Mahyar Sheikholeslami Hamid Reza Zamanizadeh Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri Journal of Crop Protection antagonist biological control cucurbits pathogen |
| title | Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri |
| title_full | Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri |
| title_fullStr | Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri |
| title_full_unstemmed | Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri |
| title_short | Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri |
| title_sort | screening of native isolates of trichoderma spp for ability to control cucumber damping off caused by phytophthora drechsleri |
| topic | antagonist biological control cucurbits pathogen |
| url | http://jcp.modares.ac.ir/article-3-41406-en.html |
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