Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions
Microalgae produce a variety of high-value products. Enhancing product contents in microalgal cells is one of the efficient ways to decrease production costs. Improved germplasm and heterotrophic cultivation may enhance microalgae biomass and lipid content. In this study, we investigated the effect...
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MDPI AG
2021-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/5/961 |
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| author | Wei Xing Ruihao Zhang Qun Shao Chunxiao Meng Xiaodong Wang Zuoxi Wei Fengjie Sun Chang Wang Kai Cao Bingkui Zhu Zhengquan Gao |
| author_facet | Wei Xing Ruihao Zhang Qun Shao Chunxiao Meng Xiaodong Wang Zuoxi Wei Fengjie Sun Chang Wang Kai Cao Bingkui Zhu Zhengquan Gao |
| author_sort | Wei Xing |
| collection | DOAJ |
| description | Microalgae produce a variety of high-value products. Enhancing product contents in microalgal cells is one of the efficient ways to decrease production costs. Improved germplasm and heterotrophic cultivation may enhance microalgae biomass and lipid content. In this study, we investigated the effect of three types of laser irradiation and heterotrophic cultivation on lipid productivity, lipid content, and biomass of two <i>Chlorella</i> strains (i.e., FACHB 9 and FACHB 31). Results showed that the highest biomasses of 4.81 g/L (15.03-fold) and 4.66 g/L (7.32-fold) were obtained in the third generation of FACHB 9 and FACHB 31 induced by a neodymium-doped yttrium aluminum garnet (Nd:YAG) laser for 8 min and 12 min, respectively. The highest lipid contents were 525.6 mg/g (1.67-fold) dry weight (DW) and 780.0 mg/g DW (2.20-fold) in the third and the first generations of FACHB 9 and FACHB 31 induced by Nd:YAG for 8 min and by a helium–neon (He–Ne) laser for 4 min, respectively. The highest lipid productivities of 69.82 ± 3.29 mg/L/d (19.7-fold) and 30.71 ± 3.77 mg/L/d (3.1-fold) were obtained in FACHB 9 and FACHB 31 treated by a semiconductor (SC) laser for 4 min and by a He–Ne laser for 12 min, respectively. Our study suggested that laser mutagenesis is a potential method for screening economically important oleaginous microalgae strains. |
| first_indexed | 2024-03-10T11:29:47Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T11:29:47Z |
| publishDate | 2021-05-01 |
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| series | Agronomy |
| spelling | doaj.art-7f03d419f0ff402fbfeece746fe885b82023-11-21T19:22:38ZengMDPI AGAgronomy2073-43952021-05-0111596110.3390/agronomy11050961Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic ConditionsWei Xing0Ruihao Zhang1Qun Shao2Chunxiao Meng3Xiaodong Wang4Zuoxi Wei5Fengjie Sun6Chang Wang7Kai Cao8Bingkui Zhu9Zhengquan Gao10School of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences, Shandong Normal University, Jinan 250014, ChinaSchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Science and Technology, Georgia Gwinnett College, 1000 University Center Lane, Lawrenceville, GA 30043, USASchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences, Shandong University of Technology, Zibo 255049, ChinaMicroalgae produce a variety of high-value products. Enhancing product contents in microalgal cells is one of the efficient ways to decrease production costs. Improved germplasm and heterotrophic cultivation may enhance microalgae biomass and lipid content. In this study, we investigated the effect of three types of laser irradiation and heterotrophic cultivation on lipid productivity, lipid content, and biomass of two <i>Chlorella</i> strains (i.e., FACHB 9 and FACHB 31). Results showed that the highest biomasses of 4.81 g/L (15.03-fold) and 4.66 g/L (7.32-fold) were obtained in the third generation of FACHB 9 and FACHB 31 induced by a neodymium-doped yttrium aluminum garnet (Nd:YAG) laser for 8 min and 12 min, respectively. The highest lipid contents were 525.6 mg/g (1.67-fold) dry weight (DW) and 780.0 mg/g DW (2.20-fold) in the third and the first generations of FACHB 9 and FACHB 31 induced by Nd:YAG for 8 min and by a helium–neon (He–Ne) laser for 4 min, respectively. The highest lipid productivities of 69.82 ± 3.29 mg/L/d (19.7-fold) and 30.71 ± 3.77 mg/L/d (3.1-fold) were obtained in FACHB 9 and FACHB 31 treated by a semiconductor (SC) laser for 4 min and by a He–Ne laser for 12 min, respectively. Our study suggested that laser mutagenesis is a potential method for screening economically important oleaginous microalgae strains.https://www.mdpi.com/2073-4395/11/5/961biomassheterotrophic cultivationlaser mutationlipid contentlipid productivitysecondary metabolite |
| spellingShingle | Wei Xing Ruihao Zhang Qun Shao Chunxiao Meng Xiaodong Wang Zuoxi Wei Fengjie Sun Chang Wang Kai Cao Bingkui Zhu Zhengquan Gao Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions Agronomy biomass heterotrophic cultivation laser mutation lipid content lipid productivity secondary metabolite |
| title | Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions |
| title_full | Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions |
| title_fullStr | Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions |
| title_full_unstemmed | Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions |
| title_short | Effects of Laser Mutagenesis on Microalgae Production and Lipid Accumulation in Two Economically Important Fresh <i>Chlorella</i> Strains under Heterotrophic Conditions |
| title_sort | effects of laser mutagenesis on microalgae production and lipid accumulation in two economically important fresh i chlorella i strains under heterotrophic conditions |
| topic | biomass heterotrophic cultivation laser mutation lipid content lipid productivity secondary metabolite |
| url | https://www.mdpi.com/2073-4395/11/5/961 |
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