Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes
Metal transporters, including divalent metal-ion transporter-1 (DMT1), Zrt-/Irt-like protein 8 and 14 (ZIP8 and ZIP14), and ferroportin-1 (FPN1), reportedly participate in cellular cadmium (Cd) uptake, but those in farm animals remain unclarified. This study aimed to examine the growth, plasma bioch...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/11/1408 |
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| author | Kefyalew Gebeyew Chunyu Jiang Qinghua Gao Liping Zhang Hanhua Zhu Yushi Tian Qi Wang Yuqing Wei Zhiliang Tan Xuefeng Han |
| author_facet | Kefyalew Gebeyew Chunyu Jiang Qinghua Gao Liping Zhang Hanhua Zhu Yushi Tian Qi Wang Yuqing Wei Zhiliang Tan Xuefeng Han |
| author_sort | Kefyalew Gebeyew |
| collection | DOAJ |
| description | Metal transporters, including divalent metal-ion transporter-1 (DMT1), Zrt-/Irt-like protein 8 and 14 (ZIP8 and ZIP14), and ferroportin-1 (FPN1), reportedly participate in cellular cadmium (Cd) uptake, but those in farm animals remain unclarified. This study aimed to examine the growth, plasma biochemical indices, Cd accumulation, and expression of metal transporter genes in the liver, kidney, and muscle of goats exposed to rice paddies contaminated with different levels of Cd. Twenty-four goats were randomly assigned across three dietary treatments: 0.23, 0.63, and 1.07 mg of Cd/kg of dry matter (DM) for 60 days. The results showed that dietary Cd exposure increased (<i>p</i> < 0.05) both Cd accumulation and the mRNA expressions of metal transporter genes (<i>DMT1</i>, <i>ZIP</i>, and <i>FPN1</i>) in the liver and kidney but not in the muscle, suggesting dietary Cd exhibited different deposition rates between goat liver, kidney, and muscle. These outcomes suggest that high levels of dietary Cd stimulated the expression of metal transporter genes and thereby enhanced the uptake and accumulation of Cd in the goat liver and kidney. As such, higher Cd concentrations in the liver and kidney observed with Cd diets could be partly explained by upregulation of metal transport genes expression. |
| first_indexed | 2024-03-10T01:33:39Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T01:33:39Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-7f098f316c004cefb295583260f024112023-11-23T13:37:56ZengMDPI AGAnimals2076-26152022-05-011211140810.3390/ani12111408Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter GenesKefyalew Gebeyew0Chunyu Jiang1Qinghua Gao2Liping Zhang3Hanhua Zhu4Yushi Tian5Qi Wang6Yuqing Wei7Zhiliang Tan8Xuefeng Han9Key Laboratory of Animal Husbandry Science and Technology of Xinjiang Production and Construction Corps, College of Animal Science, Tarim University, Alar 843300, ChinaKey Laboratory of Animal Husbandry Science and Technology of Xinjiang Production and Construction Corps, College of Animal Science, Tarim University, Alar 843300, ChinaKey Laboratory of Animal Husbandry Science and Technology of Xinjiang Production and Construction Corps, College of Animal Science, Tarim University, Alar 843300, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, ChinaMetal transporters, including divalent metal-ion transporter-1 (DMT1), Zrt-/Irt-like protein 8 and 14 (ZIP8 and ZIP14), and ferroportin-1 (FPN1), reportedly participate in cellular cadmium (Cd) uptake, but those in farm animals remain unclarified. This study aimed to examine the growth, plasma biochemical indices, Cd accumulation, and expression of metal transporter genes in the liver, kidney, and muscle of goats exposed to rice paddies contaminated with different levels of Cd. Twenty-four goats were randomly assigned across three dietary treatments: 0.23, 0.63, and 1.07 mg of Cd/kg of dry matter (DM) for 60 days. The results showed that dietary Cd exposure increased (<i>p</i> < 0.05) both Cd accumulation and the mRNA expressions of metal transporter genes (<i>DMT1</i>, <i>ZIP</i>, and <i>FPN1</i>) in the liver and kidney but not in the muscle, suggesting dietary Cd exhibited different deposition rates between goat liver, kidney, and muscle. These outcomes suggest that high levels of dietary Cd stimulated the expression of metal transporter genes and thereby enhanced the uptake and accumulation of Cd in the goat liver and kidney. As such, higher Cd concentrations in the liver and kidney observed with Cd diets could be partly explained by upregulation of metal transport genes expression.https://www.mdpi.com/2076-2615/12/11/1408cadmiumgene expressiongoatliverkidneymetal transporter |
| spellingShingle | Kefyalew Gebeyew Chunyu Jiang Qinghua Gao Liping Zhang Hanhua Zhu Yushi Tian Qi Wang Yuqing Wei Zhiliang Tan Xuefeng Han Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes Animals cadmium gene expression goat liver kidney metal transporter |
| title | Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes |
| title_full | Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes |
| title_fullStr | Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes |
| title_full_unstemmed | Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes |
| title_short | Cadmium Accumulation in the Goat Liver and Kidney Is Partially Promoted by the Upregulation of Metal Transporter Genes |
| title_sort | cadmium accumulation in the goat liver and kidney is partially promoted by the upregulation of metal transporter genes |
| topic | cadmium gene expression goat liver kidney metal transporter |
| url | https://www.mdpi.com/2076-2615/12/11/1408 |
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