Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MS
Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septa...
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MDPI AG
2019-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/12/2330 |
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| author | Jenni Tienaho Maarit Karonen Riina Muilu–Mäkelä Kristiina Wähälä Eduardo Leon Denegri Robert Franzén Matti Karp Ville Santala Tytti Sarjala |
| author_facet | Jenni Tienaho Maarit Karonen Riina Muilu–Mäkelä Kristiina Wähälä Eduardo Leon Denegri Robert Franzén Matti Karp Ville Santala Tytti Sarjala |
| author_sort | Jenni Tienaho |
| collection | DOAJ |
| description | Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC−DAD−ESI−MS−Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance. |
| first_indexed | 2024-04-12T01:21:07Z |
| format | Article |
| id | doaj.art-7f0a9b04afeb4f7d800e828b6a9d1225 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T01:21:07Z |
| publishDate | 2019-06-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7f0a9b04afeb4f7d800e828b6a9d12252022-12-22T03:53:48ZengMDPI AGMolecules1420-30492019-06-012412233010.3390/molecules24122330molecules24122330Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MSJenni Tienaho0Maarit Karonen1Riina Muilu–Mäkelä2Kristiina Wähälä3Eduardo Leon Denegri4Robert Franzén5Matti Karp6Ville Santala7Tytti Sarjala8Faculty of Natural Sciences and Engineering, Tampere University, FI-33101 Tampere, FinlandNatural Chemistry Research Group, Department of Chemistry, University of Turku, FI-20014 Turku, FinlandNatural Resources Institute Finland (Luke), FI-00791 Helsinki, FinlandDepartment of Chemistry, University of Helsinki, FI-00014 Helsinki, FinlandDepartment of Chemistry, University of Helsinki, FI-00014 Helsinki, FinlandSchool of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, FI-00076 Espoo, FinlandFaculty of Natural Sciences and Engineering, Tampere University, FI-33101 Tampere, FinlandFaculty of Natural Sciences and Engineering, Tampere University, FI-33101 Tampere, FinlandNatural Resources Institute Finland (Luke), FI-00791 Helsinki, FinlandEndophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC−DAD−ESI−MS−Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.https://www.mdpi.com/1420-3049/24/12/2330endophytesendophytic fungi<i>Acephala applanata</i><i>Phialocephala fortinii</i><i>Humicolopsis cephalosporioides</i><i>Coniochaeta mutabilis</i>Scots pinemetabolitesUPLC–MSpeptides |
| spellingShingle | Jenni Tienaho Maarit Karonen Riina Muilu–Mäkelä Kristiina Wähälä Eduardo Leon Denegri Robert Franzén Matti Karp Ville Santala Tytti Sarjala Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MS Molecules endophytes endophytic fungi <i>Acephala applanata</i> <i>Phialocephala fortinii</i> <i>Humicolopsis cephalosporioides</i> <i>Coniochaeta mutabilis</i> Scots pine metabolites UPLC–MS peptides |
| title | Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MS |
| title_full | Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MS |
| title_fullStr | Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MS |
| title_full_unstemmed | Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MS |
| title_short | Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (<i>Pinus sylvestris</i> L.) Seedlings Using UPLC–Orbitrap–MS |
| title_sort | metabolic profiling of water soluble compounds from the extracts of dark septate endophytic fungi dse isolated from scots pine i pinus sylvestris i l seedlings using uplc orbitrap ms |
| topic | endophytes endophytic fungi <i>Acephala applanata</i> <i>Phialocephala fortinii</i> <i>Humicolopsis cephalosporioides</i> <i>Coniochaeta mutabilis</i> Scots pine metabolites UPLC–MS peptides |
| url | https://www.mdpi.com/1420-3049/24/12/2330 |
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