Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases
Group B <i>Streptococcus</i> (GBS) is an important pathogen of neonatal infections, and the clonal complex (CC)-17/serotype III GBS strain has emerged as the dominant strain. The clinical manifestations of CC17/III GBS sepsis may vary greatly but have not been well-investigated. A total...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11626 |
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| author | Jen-Fu Hsu Jang-Jih Lu Chih Lin Shih-Ming Chu Lee-Chung Lin Mei-Yin Lai Hsuan-Rong Huang Ming-Chou Chiang Ming-Horng Tsai |
| author_facet | Jen-Fu Hsu Jang-Jih Lu Chih Lin Shih-Ming Chu Lee-Chung Lin Mei-Yin Lai Hsuan-Rong Huang Ming-Chou Chiang Ming-Horng Tsai |
| author_sort | Jen-Fu Hsu |
| collection | DOAJ |
| description | Group B <i>Streptococcus</i> (GBS) is an important pathogen of neonatal infections, and the clonal complex (CC)-17/serotype III GBS strain has emerged as the dominant strain. The clinical manifestations of CC17/III GBS sepsis may vary greatly but have not been well-investigated. A total of 103 CC17/III GBS isolates that caused neonatal invasive diseases were studied using a new approach based on clustered regularly interspaced short palindromic repeats (CRISPR) loci and restriction fragment length polymorphism (RFLP) analyses. All spacers of CRISPR loci were sequenced and analyzed with the clinical presentations. After CRISPR-RFLP analyses, a total of 11 different patterns were observed among the 103 CRISPR-positive GBS isolates. GBS isolates with the same RFLP patterns were found to have highly comparable spacer contents. Comparative sequence analysis of the CRISPR1 spacer content revealed that it is highly diverse and consistent with the dynamics of this system. A total of 29 of 43 (67.4%) spacers displayed homology to reported phage and plasmid DNA sequences. In addition, all CC17/III GBS isolates could be categorized into three subgroups based on the CRISPR-RFLP patterns and eBURST analysis. The CC17/III GBS isolates with a specific CRISPR-RFLP pattern were more significantly associated with occurrences of severe sepsis (57.1% vs. 29.3%, <i>p</i> = 0.012) and meningitis (50.0% vs. 20.8%, <i>p</i> = 0.009) than GBS isolates with RFLP lengths between 1000 and 1300 bp. Whole-genome sequencing was also performed to verify the differences between CC17/III GBS isolates with different CRISPR-RFLP patterns. We concluded that the CRISPR-RFLP analysis is potentially applicable to categorizing CC17/III GBS isolates, and a specific CRISPR-RFLP pattern could be used as a new biomarker to predict meningitis and illness severity after further verification. |
| first_indexed | 2024-03-10T06:01:45Z |
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| language | English |
| last_indexed | 2024-03-10T06:01:45Z |
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| spelling | doaj.art-7f0b4f77ba79480596f21f4377a55b192023-11-22T20:55:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122211162610.3390/ijms222111626Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive DiseasesJen-Fu Hsu0Jang-Jih Lu1Chih Lin2Shih-Ming Chu3Lee-Chung Lin4Mei-Yin Lai5Hsuan-Rong Huang6Ming-Chou Chiang7Ming-Horng Tsai8Division of Pediatric Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanSchool of Medicine, College of Medicine, Chang Gung University, Taoyuan 333, TaiwanDivision of Pediatric Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanDivision of Pediatric Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanDivision of Pediatric Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanDivision of Pediatric Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanDivision of Pediatric Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333, TaiwanSchool of Medicine, College of Medicine, Chang Gung University, Taoyuan 333, TaiwanGroup B <i>Streptococcus</i> (GBS) is an important pathogen of neonatal infections, and the clonal complex (CC)-17/serotype III GBS strain has emerged as the dominant strain. The clinical manifestations of CC17/III GBS sepsis may vary greatly but have not been well-investigated. A total of 103 CC17/III GBS isolates that caused neonatal invasive diseases were studied using a new approach based on clustered regularly interspaced short palindromic repeats (CRISPR) loci and restriction fragment length polymorphism (RFLP) analyses. All spacers of CRISPR loci were sequenced and analyzed with the clinical presentations. After CRISPR-RFLP analyses, a total of 11 different patterns were observed among the 103 CRISPR-positive GBS isolates. GBS isolates with the same RFLP patterns were found to have highly comparable spacer contents. Comparative sequence analysis of the CRISPR1 spacer content revealed that it is highly diverse and consistent with the dynamics of this system. A total of 29 of 43 (67.4%) spacers displayed homology to reported phage and plasmid DNA sequences. In addition, all CC17/III GBS isolates could be categorized into three subgroups based on the CRISPR-RFLP patterns and eBURST analysis. The CC17/III GBS isolates with a specific CRISPR-RFLP pattern were more significantly associated with occurrences of severe sepsis (57.1% vs. 29.3%, <i>p</i> = 0.012) and meningitis (50.0% vs. 20.8%, <i>p</i> = 0.009) than GBS isolates with RFLP lengths between 1000 and 1300 bp. Whole-genome sequencing was also performed to verify the differences between CC17/III GBS isolates with different CRISPR-RFLP patterns. We concluded that the CRISPR-RFLP analysis is potentially applicable to categorizing CC17/III GBS isolates, and a specific CRISPR-RFLP pattern could be used as a new biomarker to predict meningitis and illness severity after further verification.https://www.mdpi.com/1422-0067/22/21/11626group B <i>Streptococcus</i>CRISPRmultilocus sequence typingantimicrobial resistancephage |
| spellingShingle | Jen-Fu Hsu Jang-Jih Lu Chih Lin Shih-Ming Chu Lee-Chung Lin Mei-Yin Lai Hsuan-Rong Huang Ming-Chou Chiang Ming-Horng Tsai Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases International Journal of Molecular Sciences group B <i>Streptococcus</i> CRISPR multilocus sequence typing antimicrobial resistance phage |
| title | Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases |
| title_full | Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases |
| title_fullStr | Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases |
| title_full_unstemmed | Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases |
| title_short | Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases |
| title_sort | clustered regularly interspaced short palindromic repeat analysis of clonal complex 17 serotype iii group b i streptococcus i strains causing neonatal invasive diseases |
| topic | group B <i>Streptococcus</i> CRISPR multilocus sequence typing antimicrobial resistance phage |
| url | https://www.mdpi.com/1422-0067/22/21/11626 |
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