Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells
Abstract Breast cancer is the most prevalent malignant tumor affecting women's health. Bone is the most common distant metastatic organ, worsening the quality of life and increasing the mortality of patients. Early detection of breast cancer bone metastasis is urgent for halting disease progres...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Bioengineering & Translational Medicine |
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| Online Access: | https://doi.org/10.1002/btm2.10597 |
| _version_ | 1797363884194004992 |
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| author | Bowen Weng Mei Li Weilai Zhu Jing Peng Xufeng Mao Yanan Zheng Chi Zhang Senhao Pan Haijiao Mao Jiyuan Zhao |
| author_facet | Bowen Weng Mei Li Weilai Zhu Jing Peng Xufeng Mao Yanan Zheng Chi Zhang Senhao Pan Haijiao Mao Jiyuan Zhao |
| author_sort | Bowen Weng |
| collection | DOAJ |
| description | Abstract Breast cancer is the most prevalent malignant tumor affecting women's health. Bone is the most common distant metastatic organ, worsening the quality of life and increasing the mortality of patients. Early detection of breast cancer bone metastasis is urgent for halting disease progression and improving tumor prognosis. Recently, extracellular matrix (ECM) with biomimetic tissue niches opened a new avenue for tumor models in vitro. Here, we developed a biomimetic decellularized ECM (dECM) system to recapitulate bone niches at different situations, bone mimetic dECM from osteoblasts (BM‐ECM) and bone tumor mimetic dECM from osteosarcoma cells (OS‐ECM). The two kinds of dECMs exhibited distinct morphology, protein composition, and distribution. Interestingly, highly metastatic breast cancer cells tended to adhere and migrate on BM‐ECM, while lowly metastatic breast cancer cells preferred the OS‐ECM niche. Epithelial‐to‐mesenchymal transition was a potential mechanism to initiate the breast cancer cell migration on different biomimetic dECMs. Importantly, in the nude mice model, the dECM system captured metastatic breast cancer cells as early as 10 days after orthotopic transplantation in mammary gland pads, with higher signal on BM‐ECM than that on OS‐ECM. Collectively, the biomimetic dECM system might be a promising tumor model to distinguish the metastatic ability of breast cancer cells in vitro and to facilitate early detection of metastatic breast cancer cells in vivo, contributing to the diagnosis of breast cancer bone metastasis. |
| first_indexed | 2024-03-08T16:27:22Z |
| format | Article |
| id | doaj.art-7f0b735c4aa24dcfa9d4e44738cb9812 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-03-08T16:27:22Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-7f0b735c4aa24dcfa9d4e44738cb98122024-01-07T04:15:59ZengWileyBioengineering & Translational Medicine2380-67612024-01-0191n/an/a10.1002/btm2.10597Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cellsBowen Weng0Mei Li1Weilai Zhu2Jing Peng3Xufeng Mao4Yanan Zheng5Chi Zhang6Senhao Pan7Haijiao Mao8Jiyuan Zhao9Zhejiang Key Laboratory of Pathophysiology School of Medicine, Ningbo University Ningbo Zhejiang ChinaKey Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province The First Affiliated Hospital of Ningbo University Ningbo Zhejiang ChinaZhejiang Key Laboratory of Pathophysiology School of Medicine, Ningbo University Ningbo Zhejiang ChinaZhejiang Key Laboratory of Pathophysiology School of Medicine, Ningbo University Ningbo Zhejiang ChinaZhejiang Key Laboratory of Pathophysiology School of Medicine, Ningbo University Ningbo Zhejiang ChinaZhejiang Key Laboratory of Pathophysiology School of Medicine, Ningbo University Ningbo Zhejiang ChinaDepartment of Orthopaedic Surgery The First Affiliated Hospital of Ningbo University Ningbo Zhejiang ChinaZhejiang Key Laboratory of Pathophysiology School of Medicine, Ningbo University Ningbo Zhejiang ChinaDepartment of Orthopaedic Surgery The First Affiliated Hospital of Ningbo University Ningbo Zhejiang ChinaZhejiang Key Laboratory of Pathophysiology School of Medicine, Ningbo University Ningbo Zhejiang ChinaAbstract Breast cancer is the most prevalent malignant tumor affecting women's health. Bone is the most common distant metastatic organ, worsening the quality of life and increasing the mortality of patients. Early detection of breast cancer bone metastasis is urgent for halting disease progression and improving tumor prognosis. Recently, extracellular matrix (ECM) with biomimetic tissue niches opened a new avenue for tumor models in vitro. Here, we developed a biomimetic decellularized ECM (dECM) system to recapitulate bone niches at different situations, bone mimetic dECM from osteoblasts (BM‐ECM) and bone tumor mimetic dECM from osteosarcoma cells (OS‐ECM). The two kinds of dECMs exhibited distinct morphology, protein composition, and distribution. Interestingly, highly metastatic breast cancer cells tended to adhere and migrate on BM‐ECM, while lowly metastatic breast cancer cells preferred the OS‐ECM niche. Epithelial‐to‐mesenchymal transition was a potential mechanism to initiate the breast cancer cell migration on different biomimetic dECMs. Importantly, in the nude mice model, the dECM system captured metastatic breast cancer cells as early as 10 days after orthotopic transplantation in mammary gland pads, with higher signal on BM‐ECM than that on OS‐ECM. Collectively, the biomimetic dECM system might be a promising tumor model to distinguish the metastatic ability of breast cancer cells in vitro and to facilitate early detection of metastatic breast cancer cells in vivo, contributing to the diagnosis of breast cancer bone metastasis.https://doi.org/10.1002/btm2.10597breast cancer bone metastasisdecellularized extracellular matrix (ECM)early metastatic diagnosisin vitro tumor modelnative niches |
| spellingShingle | Bowen Weng Mei Li Weilai Zhu Jing Peng Xufeng Mao Yanan Zheng Chi Zhang Senhao Pan Haijiao Mao Jiyuan Zhao Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells Bioengineering & Translational Medicine breast cancer bone metastasis decellularized extracellular matrix (ECM) early metastatic diagnosis in vitro tumor model native niches |
| title | Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells |
| title_full | Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells |
| title_fullStr | Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells |
| title_full_unstemmed | Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells |
| title_short | Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells |
| title_sort | distinguished biomimetic decm system facilitates early detection of metastatic breast cancer cells |
| topic | breast cancer bone metastasis decellularized extracellular matrix (ECM) early metastatic diagnosis in vitro tumor model native niches |
| url | https://doi.org/10.1002/btm2.10597 |
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