Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy
Recently, we developed an integrated optical-resolution (OR) and acoustic-resolution (AR) PAM, which has multiscale imaging capability using different resolutions. However, limited by the scanning method, a tradeoff exists between the imaging speed and field of view, which impedes its wider applicat...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221359792200012X |
| _version_ | 1818006193073815552 |
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| author | Zhiqiang Xu Yinhao Pan Ningbo Chen Silue Zeng Liangjian Liu Rongkang Gao Jianhui Zhang Chihua Fang Liang Song Chengbo Liu |
| author_facet | Zhiqiang Xu Yinhao Pan Ningbo Chen Silue Zeng Liangjian Liu Rongkang Gao Jianhui Zhang Chihua Fang Liang Song Chengbo Liu |
| author_sort | Zhiqiang Xu |
| collection | DOAJ |
| description | Recently, we developed an integrated optical-resolution (OR) and acoustic-resolution (AR) PAM, which has multiscale imaging capability using different resolutions. However, limited by the scanning method, a tradeoff exists between the imaging speed and field of view, which impedes its wider applications. Here, we present an improved multiscale PAM which achieves high-speed wide-field imaging based on a homemade polygon scanner. Encoder trigger mode was proposed to avoid jittering of the polygon scanner during imaging. Distortions caused by polygon scanning were analyzed theoretically and compared with traditional types of distortions in optical-scanning PAM. Then a depth correction method was proposed and verified to compensate for the distortions. System characterization of OR-PAM and AR-PAM was performed prior to in vivo imaging. Blood reperfusion of an in vivo mouse ear was imaged continuously to demonstrate the feasibility of the multiscale PAM for high-speed imaging. Results showed that the maximum B-scan rate could be 14.65 Hz in a fixed range of 10 mm. Compared with our previous multiscale system, the imaging speed of the improved system was increased by a factor of 12.35. In vivo imaging of a subcutaneously inoculated B-16 melanoma of a mouse was performed. Results showed that the blood vasculature around the melanoma could be resolved and the melanoma could be visualized at a depth up to 1.6 mm using the multiscale PAM. |
| first_indexed | 2024-04-14T04:57:08Z |
| format | Article |
| id | doaj.art-7f0b7678369b485e8e21cd8aabf7d139 |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-04-14T04:57:08Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-7f0b7678369b485e8e21cd8aabf7d1392022-12-22T02:11:07ZengElsevierPhotoacoustics2213-59792022-06-0126100342Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopyZhiqiang Xu0Yinhao Pan1Ningbo Chen2Silue Zeng3Liangjian Liu4Rongkang Gao5Jianhui Zhang6Chihua Fang7Liang Song8Chengbo Liu9Research Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; College of Mechanical and Electrical Engineering, Guangzhou University, Guangzhou 510006, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Department of Hepatobiliary Surgery I, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaCollege of Mechanical and Electrical Engineering, Guangzhou University, Guangzhou 510006, ChinaDepartment of Hepatobiliary Surgery I, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Corresponding author.Recently, we developed an integrated optical-resolution (OR) and acoustic-resolution (AR) PAM, which has multiscale imaging capability using different resolutions. However, limited by the scanning method, a tradeoff exists between the imaging speed and field of view, which impedes its wider applications. Here, we present an improved multiscale PAM which achieves high-speed wide-field imaging based on a homemade polygon scanner. Encoder trigger mode was proposed to avoid jittering of the polygon scanner during imaging. Distortions caused by polygon scanning were analyzed theoretically and compared with traditional types of distortions in optical-scanning PAM. Then a depth correction method was proposed and verified to compensate for the distortions. System characterization of OR-PAM and AR-PAM was performed prior to in vivo imaging. Blood reperfusion of an in vivo mouse ear was imaged continuously to demonstrate the feasibility of the multiscale PAM for high-speed imaging. Results showed that the maximum B-scan rate could be 14.65 Hz in a fixed range of 10 mm. Compared with our previous multiscale system, the imaging speed of the improved system was increased by a factor of 12.35. In vivo imaging of a subcutaneously inoculated B-16 melanoma of a mouse was performed. Results showed that the blood vasculature around the melanoma could be resolved and the melanoma could be visualized at a depth up to 1.6 mm using the multiscale PAM.http://www.sciencedirect.com/science/article/pii/S221359792200012XMultiscale photoacoustic microscopyHigh speedPolygon scannerDistortion correctionMelanoma imaging |
| spellingShingle | Zhiqiang Xu Yinhao Pan Ningbo Chen Silue Zeng Liangjian Liu Rongkang Gao Jianhui Zhang Chihua Fang Liang Song Chengbo Liu Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy Photoacoustics Multiscale photoacoustic microscopy High speed Polygon scanner Distortion correction Melanoma imaging |
| title | Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy |
| title_full | Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy |
| title_fullStr | Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy |
| title_full_unstemmed | Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy |
| title_short | Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy |
| title_sort | visualizing tumor angiogenesis and boundary with polygon scanning multiscale photoacoustic microscopy |
| topic | Multiscale photoacoustic microscopy High speed Polygon scanner Distortion correction Melanoma imaging |
| url | http://www.sciencedirect.com/science/article/pii/S221359792200012X |
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