Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, Iran
Introduction: Yersinia ruckeri is the etiological agent of enteric redmouth (ERM) disease, one of the important bacterial diseases in the cultured salmonids. The aim of present study was detection of virulence genes (yrpE and yrp1) in the Y.ruckeri bacterium Materials and methods In this study fish...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2014-04-01
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| Series: | Biological Journal of Microorganism |
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| Online Access: | http://uijs.ui.ac.ir/bjm/browse.php?a_id=133&slc_lang=en&sid=1&ftxt=1 |
| _version_ | 1818208437387919360 |
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| author | Firooz fadaeifard Saeed Simin |
| author_facet | Firooz fadaeifard Saeed Simin |
| author_sort | Firooz fadaeifard |
| collection | DOAJ |
| description | Introduction: Yersinia ruckeri is the etiological agent of enteric redmouth (ERM) disease, one of the important bacterial diseases in the cultured salmonids. The aim of present study was detection of virulence genes (yrpE and yrp1) in the Y.ruckeri bacterium
Materials and methods In this study fish were evaluated in average size 10-12 Cm from six rainbow trout farms in Chaharmahal-Va-Bakhtiary province. In each farm 10 fish (totally 60) suspected to ERM were randomly selected, sampling was done from lower part of intestine and cultured on general (trypticase soy agar) and specific (Waltman-Shots) mediums. Finally, colonies that were isolated on these mediums tested by PCR method for Y.ruckeri detection. Then, in the identified strains of Y.ruckeri virulenc genes (yrp1and yrpE) were detected by PCR test.
Results: In the all strains of Y.ruckeri two virulence genes (yrp1and yrpE) were identified by molecular test. 24 strains out of total isolates were identified as Y.ruckeri, and among them 12 cases (50%) and 11 cases (45.83%) had yrp1 andyrpE genes, respectively. Also, 6 samples (25%) had both genes simultaneously.
Discussion and conclusion: The study revealed that virulence factor yrp1 is as extracellular protease presence in Y.ruckeri strains. This factor plays an important role in the bacterial virulence and pathogenesis of ERM disease. Data obtained in this study help to control disease especially in development of current vaccines. |
| first_indexed | 2024-12-12T04:44:48Z |
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| id | doaj.art-7f0d61a6870747a4a3fc09b0c3d0087b |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-12T04:44:48Z |
| publishDate | 2014-04-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-7f0d61a6870747a4a3fc09b0c3d0087b2022-12-22T00:37:39ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812014-04-01396574Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, IranFirooz fadaeifard 0Saeed Simin 1Associated professor of Aquatic animal health and disease, Shahrekord Branch,Islamic Azad University, Shahrekord, Iran, fadaeifard@gmail.comStudent of veterinary medicine, Shahrekord Branch,Islamic Azad University, Shahrekord, Iran, saeed.simin_vet@yahoo.comIntroduction: Yersinia ruckeri is the etiological agent of enteric redmouth (ERM) disease, one of the important bacterial diseases in the cultured salmonids. The aim of present study was detection of virulence genes (yrpE and yrp1) in the Y.ruckeri bacterium Materials and methods In this study fish were evaluated in average size 10-12 Cm from six rainbow trout farms in Chaharmahal-Va-Bakhtiary province. In each farm 10 fish (totally 60) suspected to ERM were randomly selected, sampling was done from lower part of intestine and cultured on general (trypticase soy agar) and specific (Waltman-Shots) mediums. Finally, colonies that were isolated on these mediums tested by PCR method for Y.ruckeri detection. Then, in the identified strains of Y.ruckeri virulenc genes (yrp1and yrpE) were detected by PCR test. Results: In the all strains of Y.ruckeri two virulence genes (yrp1and yrpE) were identified by molecular test. 24 strains out of total isolates were identified as Y.ruckeri, and among them 12 cases (50%) and 11 cases (45.83%) had yrp1 andyrpE genes, respectively. Also, 6 samples (25%) had both genes simultaneously. Discussion and conclusion: The study revealed that virulence factor yrp1 is as extracellular protease presence in Y.ruckeri strains. This factor plays an important role in the bacterial virulence and pathogenesis of ERM disease. Data obtained in this study help to control disease especially in development of current vaccines.http://uijs.ui.ac.ir/bjm/browse.php?a_id=133&slc_lang=en&sid=1&ftxt=1Rainbow troutPCRYersinia ruckeriyrp1yrpE |
| spellingShingle | Firooz fadaeifard Saeed Simin Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, Iran Biological Journal of Microorganism Rainbow trout PCR Yersinia ruckeri yrp1 yrpE |
| title | Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, Iran |
| title_full | Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, Iran |
| title_fullStr | Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, Iran |
| title_full_unstemmed | Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, Iran |
| title_short | Detection of virulence genes (yrp1 and yrpE) in the Yersinia ruckeri by polymerase chain reaction test in Chaharmahal-Va-Bakhtiary province, Iran |
| title_sort | detection of virulence genes yrp1 and yrpe in the yersinia ruckeri by polymerase chain reaction test in chaharmahal va bakhtiary province iran |
| topic | Rainbow trout PCR Yersinia ruckeri yrp1 yrpE |
| url | http://uijs.ui.ac.ir/bjm/browse.php?a_id=133&slc_lang=en&sid=1&ftxt=1 |
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