Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection.
OBJECTIVES: To investigate whether T-cell activation and exhaustion is linked to HCV- and HIV disease parameters in HIV/HCV infected individuals, we studied T-cell characteristics in HIV/HCV coinfected patients and controls. METHODS: 14 HIV/HCV coinfected, 19 HCV monoinfected, 10 HIV monoinfected pa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3598709?pdf=render |
| _version_ | 1811226290322145280 |
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| author | Thijs Feuth Joop E Arends Justin H Fransen Nening M Nanlohy Karel J van Erpecum Peter D Siersema Andy I M Hoepelman Debbie van Baarle |
| author_facet | Thijs Feuth Joop E Arends Justin H Fransen Nening M Nanlohy Karel J van Erpecum Peter D Siersema Andy I M Hoepelman Debbie van Baarle |
| author_sort | Thijs Feuth |
| collection | DOAJ |
| description | OBJECTIVES: To investigate whether T-cell activation and exhaustion is linked to HCV- and HIV disease parameters in HIV/HCV infected individuals, we studied T-cell characteristics in HIV/HCV coinfected patients and controls. METHODS: 14 HIV/HCV coinfected, 19 HCV monoinfected, 10 HIV monoinfected patients and 15 healthy controls were included in this cross-sectional study. Differences in expression of activation and exhaustion markers (HLA-DR, CD38, PD-1, Tim-3 and Fas) and phenotypic markers on CD4(+) and CD8(+) T-cells were analysed by flow cytometry and were related to HCV disease parameters (HCV-viremia, ALT and liver fibrosis). RESULTS: Frequencies of activated CD4(+) and CD8(+) T-cells were higher in HIV/HCV-coinfected compared to healthy controls and HCV or HIV mono-infected individuals. Coinfected patients also showed high expression of the exhaustion marker PD-1 and death receptor Fas. In contrast, the exhaustion marker Tim-3 was only elevated in HIV-monoinfected patients. T-cell activation and exhaustion were correlated with HCV-RNA, suggesting that viral antigen influences T-cell activation and exhaustion. Interestingly, increased percentages of effector CD8(+) T-cells were found in patients with severe (F3-F4) liver fibrosis compared to those with no to minimal fibrosis (F0-F2). CONCLUSIONS: HIV/HCV coinfected patients display a high level of T-cell activation and exhaustion in the peripheral blood. Our data suggest that T-cell activation and exhaustion are influenced by the level of HCV viremia. Furthermore, high percentages of cytotoxic/effector CD8(+) T-cells are associated with liver fibrosis in both HCV monoinfected and HIV/HCV coinfected patients. |
| first_indexed | 2024-04-12T09:21:38Z |
| format | Article |
| id | doaj.art-7f0f5cfd321a4ceda0beacde83ce6fe9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T09:21:38Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7f0f5cfd321a4ceda0beacde83ce6fe92022-12-22T03:38:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5930210.1371/journal.pone.0059302Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection.Thijs FeuthJoop E ArendsJustin H FransenNening M NanlohyKarel J van ErpecumPeter D SiersemaAndy I M HoepelmanDebbie van BaarleOBJECTIVES: To investigate whether T-cell activation and exhaustion is linked to HCV- and HIV disease parameters in HIV/HCV infected individuals, we studied T-cell characteristics in HIV/HCV coinfected patients and controls. METHODS: 14 HIV/HCV coinfected, 19 HCV monoinfected, 10 HIV monoinfected patients and 15 healthy controls were included in this cross-sectional study. Differences in expression of activation and exhaustion markers (HLA-DR, CD38, PD-1, Tim-3 and Fas) and phenotypic markers on CD4(+) and CD8(+) T-cells were analysed by flow cytometry and were related to HCV disease parameters (HCV-viremia, ALT and liver fibrosis). RESULTS: Frequencies of activated CD4(+) and CD8(+) T-cells were higher in HIV/HCV-coinfected compared to healthy controls and HCV or HIV mono-infected individuals. Coinfected patients also showed high expression of the exhaustion marker PD-1 and death receptor Fas. In contrast, the exhaustion marker Tim-3 was only elevated in HIV-monoinfected patients. T-cell activation and exhaustion were correlated with HCV-RNA, suggesting that viral antigen influences T-cell activation and exhaustion. Interestingly, increased percentages of effector CD8(+) T-cells were found in patients with severe (F3-F4) liver fibrosis compared to those with no to minimal fibrosis (F0-F2). CONCLUSIONS: HIV/HCV coinfected patients display a high level of T-cell activation and exhaustion in the peripheral blood. Our data suggest that T-cell activation and exhaustion are influenced by the level of HCV viremia. Furthermore, high percentages of cytotoxic/effector CD8(+) T-cells are associated with liver fibrosis in both HCV monoinfected and HIV/HCV coinfected patients.http://europepmc.org/articles/PMC3598709?pdf=render |
| spellingShingle | Thijs Feuth Joop E Arends Justin H Fransen Nening M Nanlohy Karel J van Erpecum Peter D Siersema Andy I M Hoepelman Debbie van Baarle Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection. PLoS ONE |
| title | Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection. |
| title_full | Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection. |
| title_fullStr | Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection. |
| title_full_unstemmed | Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection. |
| title_short | Complementary role of HCV and HIV in T-cell activation and exhaustion in HIV/HCV coinfection. |
| title_sort | complementary role of hcv and hiv in t cell activation and exhaustion in hiv hcv coinfection |
| url | http://europepmc.org/articles/PMC3598709?pdf=render |
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