Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei
Membrane contact sites are regions of close apposition between two organelles, typically less than 30 nanometers apart, that facilitate transfer of biomolecules. The presence of contact sites has been demonstrated in yeast, plants, and mammalian cells. Here, we investigated the presence of such cont...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-03-01
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| Series: | Pathogens |
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| Online Access: | http://www.mdpi.com/2076-0817/7/2/33 |
| _version_ | 1817989739039424512 |
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| author | Srinivasan Ramakrishnan Beejan Asady Roberto Docampo |
| author_facet | Srinivasan Ramakrishnan Beejan Asady Roberto Docampo |
| author_sort | Srinivasan Ramakrishnan |
| collection | DOAJ |
| description | Membrane contact sites are regions of close apposition between two organelles, typically less than 30 nanometers apart, that facilitate transfer of biomolecules. The presence of contact sites has been demonstrated in yeast, plants, and mammalian cells. Here, we investigated the presence of such contact sites in Trypanosoma brucei. In mammalian cells, endoplasmic reticulum-mitochondria contact sites facilitate mitochondrial uptake of Ca2+ released by the ER-located inositol 1,4,5-trisphosphate receptor (InsP3R). However, the InsP3R in trypanosomes localizes to acidocalcisomes, which serve as major Ca2+ stores in these parasites. In this work, we have used super-resolution structured illumination microscopy and electron microscopy to identify membrane contact sites that exist between acidocalcisomes and mitochondria. Furthermore, we have confirmed the close association of these organelles using proximity ligation assays. Characterization of these contact sites may be a necessary starting point towards unraveling the role of Ca2+ in regulating trypanosome bioenergetics. |
| first_indexed | 2024-04-14T00:50:51Z |
| format | Article |
| id | doaj.art-7f10b8c0cc20453f8d377cb93facde27 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-14T00:50:51Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7f10b8c0cc20453f8d377cb93facde272022-12-22T02:21:48ZengMDPI AGPathogens2076-08172018-03-01723310.3390/pathogens7020033pathogens7020033Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma bruceiSrinivasan Ramakrishnan0Beejan Asady1Roberto Docampo2Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USACenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USACenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USAMembrane contact sites are regions of close apposition between two organelles, typically less than 30 nanometers apart, that facilitate transfer of biomolecules. The presence of contact sites has been demonstrated in yeast, plants, and mammalian cells. Here, we investigated the presence of such contact sites in Trypanosoma brucei. In mammalian cells, endoplasmic reticulum-mitochondria contact sites facilitate mitochondrial uptake of Ca2+ released by the ER-located inositol 1,4,5-trisphosphate receptor (InsP3R). However, the InsP3R in trypanosomes localizes to acidocalcisomes, which serve as major Ca2+ stores in these parasites. In this work, we have used super-resolution structured illumination microscopy and electron microscopy to identify membrane contact sites that exist between acidocalcisomes and mitochondria. Furthermore, we have confirmed the close association of these organelles using proximity ligation assays. Characterization of these contact sites may be a necessary starting point towards unraveling the role of Ca2+ in regulating trypanosome bioenergetics.http://www.mdpi.com/2076-0817/7/2/33membrane contact sitescalcium signalingtrypanosomes |
| spellingShingle | Srinivasan Ramakrishnan Beejan Asady Roberto Docampo Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei Pathogens membrane contact sites calcium signaling trypanosomes |
| title | Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei |
| title_full | Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei |
| title_fullStr | Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei |
| title_full_unstemmed | Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei |
| title_short | Acidocalcisome-Mitochondrion Membrane Contact Sites in Trypanosoma brucei |
| title_sort | acidocalcisome mitochondrion membrane contact sites in trypanosoma brucei |
| topic | membrane contact sites calcium signaling trypanosomes |
| url | http://www.mdpi.com/2076-0817/7/2/33 |
| work_keys_str_mv | AT srinivasanramakrishnan acidocalcisomemitochondrionmembranecontactsitesintrypanosomabrucei AT beejanasady acidocalcisomemitochondrionmembranecontactsitesintrypanosomabrucei AT robertodocampo acidocalcisomemitochondrionmembranecontactsitesintrypanosomabrucei |