Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions
Abstract Recent advances in the expansion and directed pancreatogenic differentiation of human pluripotent stem cells (hPSCs) have intensified efforts to generate functional pancreatic islet cells, especially insulin-secreting β-cells, for cell therapies against diabetes. However, the consistent gen...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13036-017-0066-3 |
| _version_ | 1819036666515423232 |
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| author | Elena F. Jacobson Emmanuel S. Tzanakakis |
| author_facet | Elena F. Jacobson Emmanuel S. Tzanakakis |
| author_sort | Elena F. Jacobson |
| collection | DOAJ |
| description | Abstract Recent advances in the expansion and directed pancreatogenic differentiation of human pluripotent stem cells (hPSCs) have intensified efforts to generate functional pancreatic islet cells, especially insulin-secreting β-cells, for cell therapies against diabetes. However, the consistent generation of glucose-responsive insulin-releasing cells remains challenging. In this article, we first present basic concepts of pancreatic organogenesis, which frequently serves as a basis for engineering differentiation regimens. Next, past and current efforts are critically discussed for the conversion of hPSCs along pancreatic cell lineages, including endocrine β-cells and α-cells, as well as exocrine cells with emphasis placed on the later stages of commitment. Finally, major challenges and future directions are examined, such as the identification of factors for in vivo maturation, large-scale culture and post processing systems, cell loss during differentiation, culture economics, efficiency, and efficacy and exosomes and miRNAs in pancreatic differentiation. |
| first_indexed | 2024-12-21T08:09:09Z |
| format | Article |
| id | doaj.art-7f1277c7c3114cdfaaef289ecd89b6fe |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-12-21T08:09:09Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-7f1277c7c3114cdfaaef289ecd89b6fe2022-12-21T19:10:42ZengBMCJournal of Biological Engineering1754-16112017-07-0111111310.1186/s13036-017-0066-3Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directionsElena F. Jacobson0Emmanuel S. Tzanakakis1Department of Chemical and Biological Engineering, Tufts UniversityDepartment of Chemical and Biological Engineering, Tufts UniversityAbstract Recent advances in the expansion and directed pancreatogenic differentiation of human pluripotent stem cells (hPSCs) have intensified efforts to generate functional pancreatic islet cells, especially insulin-secreting β-cells, for cell therapies against diabetes. However, the consistent generation of glucose-responsive insulin-releasing cells remains challenging. In this article, we first present basic concepts of pancreatic organogenesis, which frequently serves as a basis for engineering differentiation regimens. Next, past and current efforts are critically discussed for the conversion of hPSCs along pancreatic cell lineages, including endocrine β-cells and α-cells, as well as exocrine cells with emphasis placed on the later stages of commitment. Finally, major challenges and future directions are examined, such as the identification of factors for in vivo maturation, large-scale culture and post processing systems, cell loss during differentiation, culture economics, efficiency, and efficacy and exosomes and miRNAs in pancreatic differentiation.http://link.springer.com/article/10.1186/s13036-017-0066-3Pluripotent stem cellsEmbryonic stem cellsInduced pluripotent stem cellsPancreatic differentiationEndocrine cellsExocrine cells |
| spellingShingle | Elena F. Jacobson Emmanuel S. Tzanakakis Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions Journal of Biological Engineering Pluripotent stem cells Embryonic stem cells Induced pluripotent stem cells Pancreatic differentiation Endocrine cells Exocrine cells |
| title | Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions |
| title_full | Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions |
| title_fullStr | Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions |
| title_full_unstemmed | Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions |
| title_short | Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions |
| title_sort | human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies innovations challenges and future directions |
| topic | Pluripotent stem cells Embryonic stem cells Induced pluripotent stem cells Pancreatic differentiation Endocrine cells Exocrine cells |
| url | http://link.springer.com/article/10.1186/s13036-017-0066-3 |
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