In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth
Abstract Polyethyleneglycol-coated biocompatible CuO–ZnO nanocomposites were fabricated hydrothermally varying Zn:Cu ratios as 1:1, 2:1, and 1:2, and their antibacterial activity was determined through the well diffusion method against the Gram-negative Escherichia coli , Pseudomonas aeruginosa, Kle...
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| Format: | Article |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52014-6 |
| _version_ | 1797355792619274240 |
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| author | Madara Jayanetti Charitha Thambiliyagodage Heshan Liyanaarachchi Geethma Ekanayake Amavin Mendis Leshan Usgodaarachchi |
| author_facet | Madara Jayanetti Charitha Thambiliyagodage Heshan Liyanaarachchi Geethma Ekanayake Amavin Mendis Leshan Usgodaarachchi |
| author_sort | Madara Jayanetti |
| collection | DOAJ |
| description | Abstract Polyethyleneglycol-coated biocompatible CuO–ZnO nanocomposites were fabricated hydrothermally varying Zn:Cu ratios as 1:1, 2:1, and 1:2, and their antibacterial activity was determined through the well diffusion method against the Gram-negative Escherichia coli , Pseudomonas aeruginosa, Klebsiella pneumoniae, and the Gram-positive Staphylococcus aureus. The minimum inhibitory concentration and the minimum bactericidal concentration values of the synthesized samples were determined. Subsequently, the time synergy kill assay was performed to elucidate the nature of the overall inhibitory effect against the aforementioned bacterial species. The mean zone of inhibition values for all four samples are presented. The inhibitory effect increased with increasing concentration of the nanocomposite (20, 40 and 60 mg/ml) on all the bacterial species except for S. aureus. According to the MBC/MIC ratio, ZnO was found to be bacteriostatic for E. coli and P. aeruginosa, and bactericidal for S. aureus and K. pneumoniae. Zn:Cu 2:1 was bactericidal on all bacterial species. A bacteriostatic effect was observed on E. coli and P. aeruginosa in the presence of Zn:Cu 1:1 whereas, it showed a bactericidal effect on S. aureus and K. pneumoniae. Zn:Cu 1:2 exhibited a bacteriostatic effect on E. coli while a bactericidal effect was observed for E. coli, P. aeruginosa, and K. pneumoniae. The metal oxide nanocomposites were found to be more sensitive towards the Gram-positive strain than the Gram-negative strains. Further, all the nanocomposites possess anti-oxidant activity as shown by the DPPH assay. |
| first_indexed | 2024-03-08T14:17:15Z |
| format | Article |
| id | doaj.art-7f139299baf1485ea80d07c153d494da |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T14:17:15Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7f139299baf1485ea80d07c153d494da2024-01-14T12:21:12ZengNature PortfolioScientific Reports2045-23222024-01-0114112110.1038/s41598-024-52014-6In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growthMadara Jayanetti0Charitha Thambiliyagodage1Heshan Liyanaarachchi2Geethma Ekanayake3Amavin Mendis4Leshan Usgodaarachchi5Faculty of Humanities and Sciences, Sri Lanka Institute of Information TechnologyFaculty of Humanities and Sciences, Sri Lanka Institute of Information TechnologyFaculty of Humanities and Sciences, Sri Lanka Institute of Information TechnologyFaculty of Humanities and Sciences, Sri Lanka Institute of Information TechnologyFaculty of Humanities and Sciences, Sri Lanka Institute of Information TechnologyFaculty of Humanities and Sciences, Sri Lanka Institute of Information TechnologyAbstract Polyethyleneglycol-coated biocompatible CuO–ZnO nanocomposites were fabricated hydrothermally varying Zn:Cu ratios as 1:1, 2:1, and 1:2, and their antibacterial activity was determined through the well diffusion method against the Gram-negative Escherichia coli , Pseudomonas aeruginosa, Klebsiella pneumoniae, and the Gram-positive Staphylococcus aureus. The minimum inhibitory concentration and the minimum bactericidal concentration values of the synthesized samples were determined. Subsequently, the time synergy kill assay was performed to elucidate the nature of the overall inhibitory effect against the aforementioned bacterial species. The mean zone of inhibition values for all four samples are presented. The inhibitory effect increased with increasing concentration of the nanocomposite (20, 40 and 60 mg/ml) on all the bacterial species except for S. aureus. According to the MBC/MIC ratio, ZnO was found to be bacteriostatic for E. coli and P. aeruginosa, and bactericidal for S. aureus and K. pneumoniae. Zn:Cu 2:1 was bactericidal on all bacterial species. A bacteriostatic effect was observed on E. coli and P. aeruginosa in the presence of Zn:Cu 1:1 whereas, it showed a bactericidal effect on S. aureus and K. pneumoniae. Zn:Cu 1:2 exhibited a bacteriostatic effect on E. coli while a bactericidal effect was observed for E. coli, P. aeruginosa, and K. pneumoniae. The metal oxide nanocomposites were found to be more sensitive towards the Gram-positive strain than the Gram-negative strains. Further, all the nanocomposites possess anti-oxidant activity as shown by the DPPH assay.https://doi.org/10.1038/s41598-024-52014-6 |
| spellingShingle | Madara Jayanetti Charitha Thambiliyagodage Heshan Liyanaarachchi Geethma Ekanayake Amavin Mendis Leshan Usgodaarachchi In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth Scientific Reports |
| title | In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth |
| title_full | In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth |
| title_fullStr | In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth |
| title_full_unstemmed | In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth |
| title_short | In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth |
| title_sort | in vitro influence of peg functionalized zno cuo nanocomposites on bacterial growth |
| url | https://doi.org/10.1038/s41598-024-52014-6 |
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