Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus
The increasing levels of environmental estrogens are causing negative effects on water, soil, wildlife, and human beings; label-free immunosensors with high specificities and sensitivities are being developed to test estrogeneous chemicals in complex environmental conditions. For the first time, hig...
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MDPI AG
2022-04-01
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author | Ailing Yang Yue Su Zhenzhong Zhang Huaidong Wang Chong Qi Shaoguo Ru Jun Wang |
author_facet | Ailing Yang Yue Su Zhenzhong Zhang Huaidong Wang Chong Qi Shaoguo Ru Jun Wang |
author_sort | Ailing Yang |
collection | DOAJ |
description | The increasing levels of environmental estrogens are causing negative effects on water, soil, wildlife, and human beings; label-free immunosensors with high specificities and sensitivities are being developed to test estrogeneous chemicals in complex environmental conditions. For the first time, highly fluorescent graphene quantum dots (GQDs) were prepared using a visible-Fenton catalysis reaction with graphene oxide (GO) as a precursor. Different microscopy and spectroscopy techniques were employed to characterize the physical and chemical properties of the GQDs. Based on the fluorescence resonance energy transfer (FRET) between amino-functionalized GQDs conjugated with anti-lipovitellin monoclonal antibodies (Anti-Lv-mAb) and reduced graphene oxide (rGO), an ultrasensitive fluorescent “ON-OFF” label-free immunosensor for the detection of lipovitellin (Lv), a sensitive biomarker derived from Paralichthys olivaceus for environmental estrogen, has been established. The immunosensor has a wide linear test range (0.001–1500 ng/mL), a lower limit of detection (LOD, 0.9 pg/mL), excellent sensitivity (26,407.8 CPS/(ng/mL)), and high selectivity and reproducibility for Lv quantification. The results demonstrated that the visible-Fenton is a simple, mild, green, efficient, and general approach to fabricating GQDs, and the fluorescent “ON-OFF” immunosensor is an easy-to-use, time-saving, ultrasensitive, and accurate detection method for weak estrogenic activity. |
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spelling | doaj.art-7f1750a2b2994171999410eba12117ed2023-12-01T00:58:31ZengMDPI AGBiosensors2079-63742022-04-0112424610.3390/bios12040246Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys OlivaceusAiling Yang0Yue Su1Zhenzhong Zhang2Huaidong Wang3Chong Qi4Shaoguo Ru5Jun Wang6College of Physics & Optoelectronic Engineering, Ocean University of China, Qingdao 266100, ChinaCollege of Physics & Optoelectronic Engineering, Ocean University of China, Qingdao 266100, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaCollege of Physics & Optoelectronic Engineering, Ocean University of China, Qingdao 266100, ChinaCollege of Physics & Optoelectronic Engineering, Ocean University of China, Qingdao 266100, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaThe increasing levels of environmental estrogens are causing negative effects on water, soil, wildlife, and human beings; label-free immunosensors with high specificities and sensitivities are being developed to test estrogeneous chemicals in complex environmental conditions. For the first time, highly fluorescent graphene quantum dots (GQDs) were prepared using a visible-Fenton catalysis reaction with graphene oxide (GO) as a precursor. Different microscopy and spectroscopy techniques were employed to characterize the physical and chemical properties of the GQDs. Based on the fluorescence resonance energy transfer (FRET) between amino-functionalized GQDs conjugated with anti-lipovitellin monoclonal antibodies (Anti-Lv-mAb) and reduced graphene oxide (rGO), an ultrasensitive fluorescent “ON-OFF” label-free immunosensor for the detection of lipovitellin (Lv), a sensitive biomarker derived from Paralichthys olivaceus for environmental estrogen, has been established. The immunosensor has a wide linear test range (0.001–1500 ng/mL), a lower limit of detection (LOD, 0.9 pg/mL), excellent sensitivity (26,407.8 CPS/(ng/mL)), and high selectivity and reproducibility for Lv quantification. The results demonstrated that the visible-Fenton is a simple, mild, green, efficient, and general approach to fabricating GQDs, and the fluorescent “ON-OFF” immunosensor is an easy-to-use, time-saving, ultrasensitive, and accurate detection method for weak estrogenic activity.https://www.mdpi.com/2079-6374/12/4/246graphene quantum dotsvisible-Fenton reactionfluorescence resonance energy transferimmunosensorenvironmental estrogenlipovitellin |
spellingShingle | Ailing Yang Yue Su Zhenzhong Zhang Huaidong Wang Chong Qi Shaoguo Ru Jun Wang Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus Biosensors graphene quantum dots visible-Fenton reaction fluorescence resonance energy transfer immunosensor environmental estrogen lipovitellin |
title | Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus |
title_full | Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus |
title_fullStr | Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus |
title_full_unstemmed | Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus |
title_short | Preparation of Graphene Quantum Dots by Visible-Fenton Reaction and Ultrasensitive Label-Free Immunosensor for Detecting Lipovitellin of Paralichthys Olivaceus |
title_sort | preparation of graphene quantum dots by visible fenton reaction and ultrasensitive label free immunosensor for detecting lipovitellin of paralichthys olivaceus |
topic | graphene quantum dots visible-Fenton reaction fluorescence resonance energy transfer immunosensor environmental estrogen lipovitellin |
url | https://www.mdpi.com/2079-6374/12/4/246 |
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