Effects of Copper Availability on the Physiology of Marine Heterotrophic Bacteria
Marine heterotrophic bacteria play a crucial role in the cycling of energy and nutrients in the ocean. Copper (Cu) belongs to the repertoire of essential trace nutrients for bacterial growth, yet physiological responses of marine heterotrophic bacteria to Cu deficiency remain unexplored. Here, we ex...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmars.2018.00523/full |
| _version_ | 1819084128826425344 |
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| author | Anna M. Posacka David M. Semeniuk Maria T. Maldonado |
| author_facet | Anna M. Posacka David M. Semeniuk Maria T. Maldonado |
| author_sort | Anna M. Posacka |
| collection | DOAJ |
| description | Marine heterotrophic bacteria play a crucial role in the cycling of energy and nutrients in the ocean. Copper (Cu) belongs to the repertoire of essential trace nutrients for bacterial growth, yet physiological responses of marine heterotrophic bacteria to Cu deficiency remain unexplored. Here, we examined these responses in oceanic and coastal isolates of heterotrophic bacteria from ecologically significant microbial clades (Flavobacteriia class from Bacteroidetes phylum, and marine Roseobacter clade within Alphaproteobacteria class and Alteromonadales within Gammaproteobacteria class, both from Proteobacteria phylum). Bacterial growth, Cu quotas (Cu:P), macronutrient content and stoichiometry (cellular C, N, P, S, and C:N, S:P), as well as carbon metabolism (respiration, productivity, carbon demand, growth efficiency) were monitored across a gradient of Cu conditions, characteristic of coastal and open-ocean surface waters. Cu deficiency had most severe effects on a Flavobacteriia member Dokdonia sp. strain Dokd-P16 for which we observed significant reductions in growth, C metabolism and Cu quotas. Other strains did not significantly reduce their growth rate, but adjusted their Cu content and some C metabolic rates (Ruegeria pomeroyi DSS-3, Roseobacter clade) or were unaffected (Pseudoalteromonas sp. strains PAlt-P26 and PAlt-P2, Alteromonadales clade). These diverse bacterial responses were accompanied by constant cellular composition of major elements and stoichiometric ratios. Changes in bacterial Cu quotas occurred within a modest range (∼5-fold range) relative to the 50-fold variation in total Cu in the media. We hypothesize that this may reflect a well-controlled Cu homeostasis in marine heterotrophic bacteria. In a preliminary assessment, we found that Cu quotas of bacteria and those of eukaryotic phytoplankton are not statistically different. However, compared to eukaryotic phytoplankton, the variability of Cu quotas in marine heterotrophic bacteria is smaller, which could reflect differences in their Cu homeostasis. Using Cu quotas obtained in our study, we assessed the contribution of bacterial Cu to the biogenic Cu pool in an oceanic euphotic zone in the NE Pacific. These preliminary estimates suggest that up to 50% of the biogenic Cu could be contained in the biomass of marine heterotrophic bacteria. Our study sheds light on the interactions between Cu and marine heterotrophic bacteria, demonstrating the potential for Cu to influence microbial ecology and for microbes to play role in Cu biolgeochemical cycle. |
| first_indexed | 2024-12-21T20:43:33Z |
| format | Article |
| id | doaj.art-7f2268bfb99b49c2aab5f7d556046a09 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-21T20:43:33Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-7f2268bfb99b49c2aab5f7d556046a092022-12-21T18:50:54ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452019-02-01510.3389/fmars.2018.00523397955Effects of Copper Availability on the Physiology of Marine Heterotrophic BacteriaAnna M. PosackaDavid M. SemeniukMaria T. MaldonadoMarine heterotrophic bacteria play a crucial role in the cycling of energy and nutrients in the ocean. Copper (Cu) belongs to the repertoire of essential trace nutrients for bacterial growth, yet physiological responses of marine heterotrophic bacteria to Cu deficiency remain unexplored. Here, we examined these responses in oceanic and coastal isolates of heterotrophic bacteria from ecologically significant microbial clades (Flavobacteriia class from Bacteroidetes phylum, and marine Roseobacter clade within Alphaproteobacteria class and Alteromonadales within Gammaproteobacteria class, both from Proteobacteria phylum). Bacterial growth, Cu quotas (Cu:P), macronutrient content and stoichiometry (cellular C, N, P, S, and C:N, S:P), as well as carbon metabolism (respiration, productivity, carbon demand, growth efficiency) were monitored across a gradient of Cu conditions, characteristic of coastal and open-ocean surface waters. Cu deficiency had most severe effects on a Flavobacteriia member Dokdonia sp. strain Dokd-P16 for which we observed significant reductions in growth, C metabolism and Cu quotas. Other strains did not significantly reduce their growth rate, but adjusted their Cu content and some C metabolic rates (Ruegeria pomeroyi DSS-3, Roseobacter clade) or were unaffected (Pseudoalteromonas sp. strains PAlt-P26 and PAlt-P2, Alteromonadales clade). These diverse bacterial responses were accompanied by constant cellular composition of major elements and stoichiometric ratios. Changes in bacterial Cu quotas occurred within a modest range (∼5-fold range) relative to the 50-fold variation in total Cu in the media. We hypothesize that this may reflect a well-controlled Cu homeostasis in marine heterotrophic bacteria. In a preliminary assessment, we found that Cu quotas of bacteria and those of eukaryotic phytoplankton are not statistically different. However, compared to eukaryotic phytoplankton, the variability of Cu quotas in marine heterotrophic bacteria is smaller, which could reflect differences in their Cu homeostasis. Using Cu quotas obtained in our study, we assessed the contribution of bacterial Cu to the biogenic Cu pool in an oceanic euphotic zone in the NE Pacific. These preliminary estimates suggest that up to 50% of the biogenic Cu could be contained in the biomass of marine heterotrophic bacteria. Our study sheds light on the interactions between Cu and marine heterotrophic bacteria, demonstrating the potential for Cu to influence microbial ecology and for microbes to play role in Cu biolgeochemical cycle.https://www.frontiersin.org/article/10.3389/fmars.2018.00523/fullcopperCu quotalimitationmarine heterotrophic bacteriaFlavobacteriiaRoseobacteria |
| spellingShingle | Anna M. Posacka David M. Semeniuk Maria T. Maldonado Effects of Copper Availability on the Physiology of Marine Heterotrophic Bacteria Frontiers in Marine Science copper Cu quota limitation marine heterotrophic bacteria Flavobacteriia Roseobacteria |
| title | Effects of Copper Availability on the Physiology of Marine Heterotrophic Bacteria |
| title_full | Effects of Copper Availability on the Physiology of Marine Heterotrophic Bacteria |
| title_fullStr | Effects of Copper Availability on the Physiology of Marine Heterotrophic Bacteria |
| title_full_unstemmed | Effects of Copper Availability on the Physiology of Marine Heterotrophic Bacteria |
| title_short | Effects of Copper Availability on the Physiology of Marine Heterotrophic Bacteria |
| title_sort | effects of copper availability on the physiology of marine heterotrophic bacteria |
| topic | copper Cu quota limitation marine heterotrophic bacteria Flavobacteriia Roseobacteria |
| url | https://www.frontiersin.org/article/10.3389/fmars.2018.00523/full |
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