Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin
Abstract Background In recent years, researchers have become increasingly interested in developing natural feed additives that can stabilize ruminal pH and thus prevent or eliminate the risk of severe subacute rumen acidosis. Herein, 3 experiments were conducted using a semi-automated in vitro gas p...
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03457-4 |
| _version_ | 1798001410831286272 |
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| author | Mariam G. Ahmed Adham A. Al-Sagheer Samir Z. El-Zarkouny Eman A. Elwakeel |
| author_facet | Mariam G. Ahmed Adham A. Al-Sagheer Samir Z. El-Zarkouny Eman A. Elwakeel |
| author_sort | Mariam G. Ahmed |
| collection | DOAJ |
| description | Abstract Background In recent years, researchers have become increasingly interested in developing natural feed additives that can stabilize ruminal pH and thus prevent or eliminate the risk of severe subacute rumen acidosis. Herein, 3 experiments were conducted using a semi-automated in vitro gas production technique. In the experiment (Exp.) 1, the efficacy of 9 plant extracts (1.5 mg/ml), compared to monensin (MON; 12 μg/ml), to counteract ruminal acidosis stimulated by adding glucose (0.1 g/ml) as a fermentable carbohydrate without buffer was assessed for 6 h. In Exp. 2, cinnamon extract (CIN) and MON were evaluated to combat glucose-induced acidosis with buffer use for 24 h. In Exp. 3, the effect of CIN and MON on preventing acidosis when corn or barley grains were used as substrate was examined. Results In Exp. 1, cinnamon, grape seeds, orange, pomegranate peels, propolis, and guava extracts significantly increased (P < 0.05) pH compared to control (CON). Both CIN and MON significantly increased the pH (P < 0.001) but reduced cumulated gas production (P < 0.01) compared to the other treatments. In Exp. 2, the addition of CIN extract increased (P < 0.01) pH value compared to CON at the first 6 h of incubation. However, no significant differences in pH values between CIN and CON at 24 h of incubation were observed. The addition of CIN extract and MON decreased (P < 0.001) lactic acid concentration and TVFA compared to CON at 24 h. The CIN significantly (P < 0.01) increased acetate: propionate ratio while MON reduced it. In Exp. 3, both CIN and MON significantly increased (P < 0.05) ruminal pH at 6 and 24 h and reduced lactic acid concentration at 24 h compared to CON with corn as substrate. However, CIN had no effect on pH with barley substrate at all incubation times. Conclusions It can be concluded that CIN can be used effectively as an alternative antibiotic to MON to control ruminal acidosis when corn is used as a basal diet. |
| first_indexed | 2024-04-11T11:35:53Z |
| format | Article |
| id | doaj.art-7f247023cf5c47a8820476b22eb1a830 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-11T11:35:53Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-7f247023cf5c47a8820476b22eb1a8302022-12-22T04:25:58ZengBMCBMC Veterinary Research1746-61482022-09-0118111110.1186/s12917-022-03457-4Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensinMariam G. Ahmed0Adham A. Al-Sagheer1Samir Z. El-Zarkouny2Eman A. Elwakeel3Department of Animal and Fish Production, Faculty of Agriculture (El-Shatby), Alexandria UniversityAnimal Production Department, Faculty of Agriculture, Zagazig UniversityDepartment of Animal and Fish Production, Faculty of Agriculture (El-Shatby), Alexandria UniversityDepartment of Animal and Fish Production, Faculty of Agriculture (El-Shatby), Alexandria UniversityAbstract Background In recent years, researchers have become increasingly interested in developing natural feed additives that can stabilize ruminal pH and thus prevent or eliminate the risk of severe subacute rumen acidosis. Herein, 3 experiments were conducted using a semi-automated in vitro gas production technique. In the experiment (Exp.) 1, the efficacy of 9 plant extracts (1.5 mg/ml), compared to monensin (MON; 12 μg/ml), to counteract ruminal acidosis stimulated by adding glucose (0.1 g/ml) as a fermentable carbohydrate without buffer was assessed for 6 h. In Exp. 2, cinnamon extract (CIN) and MON were evaluated to combat glucose-induced acidosis with buffer use for 24 h. In Exp. 3, the effect of CIN and MON on preventing acidosis when corn or barley grains were used as substrate was examined. Results In Exp. 1, cinnamon, grape seeds, orange, pomegranate peels, propolis, and guava extracts significantly increased (P < 0.05) pH compared to control (CON). Both CIN and MON significantly increased the pH (P < 0.001) but reduced cumulated gas production (P < 0.01) compared to the other treatments. In Exp. 2, the addition of CIN extract increased (P < 0.01) pH value compared to CON at the first 6 h of incubation. However, no significant differences in pH values between CIN and CON at 24 h of incubation were observed. The addition of CIN extract and MON decreased (P < 0.001) lactic acid concentration and TVFA compared to CON at 24 h. The CIN significantly (P < 0.01) increased acetate: propionate ratio while MON reduced it. In Exp. 3, both CIN and MON significantly increased (P < 0.05) ruminal pH at 6 and 24 h and reduced lactic acid concentration at 24 h compared to CON with corn as substrate. However, CIN had no effect on pH with barley substrate at all incubation times. Conclusions It can be concluded that CIN can be used effectively as an alternative antibiotic to MON to control ruminal acidosis when corn is used as a basal diet.https://doi.org/10.1186/s12917-022-03457-4Ruminal acidosisPlant extractsCinnamon extractMonensin |
| spellingShingle | Mariam G. Ahmed Adham A. Al-Sagheer Samir Z. El-Zarkouny Eman A. Elwakeel Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin BMC Veterinary Research Ruminal acidosis Plant extracts Cinnamon extract Monensin |
| title | Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin |
| title_full | Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin |
| title_fullStr | Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin |
| title_full_unstemmed | Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin |
| title_short | Potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin |
| title_sort | potential of selected plant extracts to control severe subacute ruminal acidosis in vitro as compared with monensin |
| topic | Ruminal acidosis Plant extracts Cinnamon extract Monensin |
| url | https://doi.org/10.1186/s12917-022-03457-4 |
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