Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran
Background and Aim: Cystic echinococcosis (CE), as a major zoonotic helminth infection, imposes remarkable socioeconomic burden on many endemic countries across the world, including Iran. Due to the high importance of free-roaming dogs in the transmission of CE, epidemiological and molecular studies...
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| Format: | Article |
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Veterinary World
2020-04-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.13/April-2020/18.pdf |
| _version_ | 1818649442525380608 |
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| author | Alireza Keyhani Iraj Sharifi Mehdi Bamorovat Mohammad Ali Mohammadi Asma Askari Mohammad Ebrahimipour Majid Fasihi Harandi |
| author_facet | Alireza Keyhani Iraj Sharifi Mehdi Bamorovat Mohammad Ali Mohammadi Asma Askari Mohammad Ebrahimipour Majid Fasihi Harandi |
| author_sort | Alireza Keyhani |
| collection | DOAJ |
| description | Background and Aim: Cystic echinococcosis (CE), as a major zoonotic helminth infection, imposes remarkable socioeconomic burden on many endemic countries across the world, including Iran. Due to the high importance of free-roaming dogs in the transmission of CE, epidemiological and molecular studies in this type of hosts are required in the endemic regions. This study aimed to investigate the epidemiology and genotyping of Echinococcus granulosus isolated from stray dogs in Kerman, Southeast Iran.
Materials and Methods: Eighty-four samples were isolated from stray dogs in the city and suburbs of Kerman in coordination with the health authorities and the municipality office for rabies control and dog population management. Dog demographic data, including age and sex were collected. The worm was isolated by necropsy and genomic DNA was extracted and partial cytochrome c oxidase subunit 1 gene was amplified using specific primers. Phylogenetic and Templeton-Crandall-Sing (TCS) network analyses were carried out on the sequence data.
Results: The overall prevalence of CE in the surveyed dogs was 10.7% (9/84 cases). Out of 84 stray dogs, 33 (39.3%) and 51 (60.7%) cases were male and female, respectively. There was not a statistically significant difference between the infection and gender of dogs. However, infection is shown more in dogs under one year of age with a statistically significant difference (p<0.05). The results of molecular studies indicated E. granulosus G1 genotype for all isolates. The high presence of free-roaming dogs in urban and peri-urban areas and high frequency of parasite in this animal is a risk factor for humans in the region. Haplotype sequence analysis on the dog isolates revealed a close relationship with other E. granulosus isolates in Kerman.
Conclusion: The findings of this study provide evidence-based data about the epidemiological and molecular characteristics of CE in dog definitive hosts of Southeast Iran. Further studies are required to understand the prevalence and parasite genotypes in dogs in Iran. |
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| id | doaj.art-7f24d5a30a5343c1a7235db08986b218 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T01:34:23Z |
| publishDate | 2020-04-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-7f24d5a30a5343c1a7235db08986b2182022-12-21T22:08:29ZengVeterinary WorldVeterinary World0972-89882231-09162020-04-0113473974510.14202/vetworld.2020.739-745Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast IranAlireza Keyhani0Iraj Sharifi1https://orcid.org/0000-0002-6894-6834Mehdi Bamorovat2Mohammad Ali Mohammadi3https://orcid.org/0000-0002-0438-4174Asma Askari4Mohammad Ebrahimipour5https://orcid.org/0000-0002-3832-3337Majid Fasihi Harandi6https://orcid.org/0000-0003-3257-5389Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.Background and Aim: Cystic echinococcosis (CE), as a major zoonotic helminth infection, imposes remarkable socioeconomic burden on many endemic countries across the world, including Iran. Due to the high importance of free-roaming dogs in the transmission of CE, epidemiological and molecular studies in this type of hosts are required in the endemic regions. This study aimed to investigate the epidemiology and genotyping of Echinococcus granulosus isolated from stray dogs in Kerman, Southeast Iran. Materials and Methods: Eighty-four samples were isolated from stray dogs in the city and suburbs of Kerman in coordination with the health authorities and the municipality office for rabies control and dog population management. Dog demographic data, including age and sex were collected. The worm was isolated by necropsy and genomic DNA was extracted and partial cytochrome c oxidase subunit 1 gene was amplified using specific primers. Phylogenetic and Templeton-Crandall-Sing (TCS) network analyses were carried out on the sequence data. Results: The overall prevalence of CE in the surveyed dogs was 10.7% (9/84 cases). Out of 84 stray dogs, 33 (39.3%) and 51 (60.7%) cases were male and female, respectively. There was not a statistically significant difference between the infection and gender of dogs. However, infection is shown more in dogs under one year of age with a statistically significant difference (p<0.05). The results of molecular studies indicated E. granulosus G1 genotype for all isolates. The high presence of free-roaming dogs in urban and peri-urban areas and high frequency of parasite in this animal is a risk factor for humans in the region. Haplotype sequence analysis on the dog isolates revealed a close relationship with other E. granulosus isolates in Kerman. Conclusion: The findings of this study provide evidence-based data about the epidemiological and molecular characteristics of CE in dog definitive hosts of Southeast Iran. Further studies are required to understand the prevalence and parasite genotypes in dogs in Iran.http://www.veterinaryworld.org/Vol.13/April-2020/18.pdfechinococcosisfree-roaming dogsgenotypehaplotype networkhydatid cystiran |
| spellingShingle | Alireza Keyhani Iraj Sharifi Mehdi Bamorovat Mohammad Ali Mohammadi Asma Askari Mohammad Ebrahimipour Majid Fasihi Harandi Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran Veterinary World echinococcosis free-roaming dogs genotype haplotype network hydatid cyst iran |
| title | Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran |
| title_full | Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran |
| title_fullStr | Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran |
| title_full_unstemmed | Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran |
| title_short | Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran |
| title_sort | epidemiological and molecular studies on echinococcus granulosus from free roaming dogs in southeast iran |
| topic | echinococcosis free-roaming dogs genotype haplotype network hydatid cyst iran |
| url | http://www.veterinaryworld.org/Vol.13/April-2020/18.pdf |
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