In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i>
Both fluorescent and luminescent observation are widely used to examine real-time gene expression patterns in living organisms. Several fluuorescent and luminescent proteins with specific optical properties have been developed and applied for simultaneous, multi-color observation of more than two ge...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/1/119 |
| _version_ | 1797543574126985216 |
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| author | Motomichi Doi Megumi Sato Yoshihiro Ohmiya |
| author_facet | Motomichi Doi Megumi Sato Yoshihiro Ohmiya |
| author_sort | Motomichi Doi |
| collection | DOAJ |
| description | Both fluorescent and luminescent observation are widely used to examine real-time gene expression patterns in living organisms. Several fluuorescent and luminescent proteins with specific optical properties have been developed and applied for simultaneous, multi-color observation of more than two gene expression profiles. Compared to fluorescent proteins, however, the application of multi-color luminescent imaging in living organisms is still limited. In this study, we introduced two-color luciferases into the soil nematode <i>C. elegans</i> and performed simultaneous analysis of two gene expression profiles. Using a green-emitting luciferase Eluc (emerald luciferase) and red-emitting luciferase SLR (stable luciferase red), the expression patterns of two genes were simultaneously observed in single animals from embryonic to adult stages over its whole life span. In addition, dual gene activities were observed at the single embryo level, with the simultaneous observation of morphological changes. These are the first application of a two-color luciferase system into a whole animal and suggest that precise relationship of expression patterns of multiple genes of interest can be analyzed over the whole life of the animal, dependent on the changes in genetic and/or environmental conditions. |
| first_indexed | 2024-03-10T13:47:34Z |
| format | Article |
| id | doaj.art-7f25b2687dbf4ff18b6762b179f6aac4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:47:34Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7f25b2687dbf4ff18b6762b179f6aac42023-11-21T02:28:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-0122111910.3390/ijms22010119In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i>Motomichi Doi0Megumi Sato1Yoshihiro Ohmiya2Biomedical Research Institute, AIST, Tsukuba, Ibaraki 305-8566, JapanBiomedical Research Institute, AIST, Tsukuba, Ibaraki 305-8566, JapanBiomedical Research Institute, AIST, Tsukuba, Ibaraki 305-8566, JapanBoth fluorescent and luminescent observation are widely used to examine real-time gene expression patterns in living organisms. Several fluuorescent and luminescent proteins with specific optical properties have been developed and applied for simultaneous, multi-color observation of more than two gene expression profiles. Compared to fluorescent proteins, however, the application of multi-color luminescent imaging in living organisms is still limited. In this study, we introduced two-color luciferases into the soil nematode <i>C. elegans</i> and performed simultaneous analysis of two gene expression profiles. Using a green-emitting luciferase Eluc (emerald luciferase) and red-emitting luciferase SLR (stable luciferase red), the expression patterns of two genes were simultaneously observed in single animals from embryonic to adult stages over its whole life span. In addition, dual gene activities were observed at the single embryo level, with the simultaneous observation of morphological changes. These are the first application of a two-color luciferase system into a whole animal and suggest that precise relationship of expression patterns of multiple genes of interest can be analyzed over the whole life of the animal, dependent on the changes in genetic and/or environmental conditions.https://www.mdpi.com/1422-0067/22/1/119bioluminescencegene expressionmulti-color luciferases<i>C. elegans</i> |
| spellingShingle | Motomichi Doi Megumi Sato Yoshihiro Ohmiya In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i> International Journal of Molecular Sciences bioluminescence gene expression multi-color luciferases <i>C. elegans</i> |
| title | In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i> |
| title_full | In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i> |
| title_fullStr | In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i> |
| title_short | In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in <i>Caenorhabditis elegans</i> |
| title_sort | in vivo simultaneous analysis of gene expression by dual color luciferases in i caenorhabditis elegans i |
| topic | bioluminescence gene expression multi-color luciferases <i>C. elegans</i> |
| url | https://www.mdpi.com/1422-0067/22/1/119 |
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