Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction.
A single HIV-1 variant establishes infection of the host after sexual contact. Identifying the phenotypic characteristics of these Transmitted Founder (T/F) viruses is important to understand the restriction mechanisms during transmission. Langerhans cells (LCs) are the mucosal dendritic cell subset...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0226651 |
| _version_ | 1819128428438224896 |
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| author | Nina Hertoghs Bernadien M Nijmeijer Nienke H van Teijlingen Angharad E Fenton-May Tanja M Kaptein John L van Hamme John C Kappes Neeltje A Kootstra Beatrice H Hahn Persephone Borrow Carla M S Ribeiro Teunis B H Geijtenbeek |
| author_facet | Nina Hertoghs Bernadien M Nijmeijer Nienke H van Teijlingen Angharad E Fenton-May Tanja M Kaptein John L van Hamme John C Kappes Neeltje A Kootstra Beatrice H Hahn Persephone Borrow Carla M S Ribeiro Teunis B H Geijtenbeek |
| author_sort | Nina Hertoghs |
| collection | DOAJ |
| description | A single HIV-1 variant establishes infection of the host after sexual contact. Identifying the phenotypic characteristics of these Transmitted Founder (T/F) viruses is important to understand the restriction mechanisms during transmission. Langerhans cells (LCs) are the mucosal dendritic cell subset that has been shown to have a protective role in HIV-1 transmission. Immature LCs efficiently capture and degrade HIV-1 via langerin-mediated restriction. Here we have investigated the capacity of T/F HIV-1 strains to infect mucosal Langerhans cells (LCs). Notably, most T/F variants efficiently infected immature LCs derived from skin and vaginal tissue in contrast to chronic HIV-1 laboratory strains. Next we screened a panel of T/F viruses and their matched 6-month consensus sequence viruses. Interestingly most T/F variants infected immature LCs whereas donor-matched 6-month consensus sequence viruses had lost the ability to infect LCs. However, we also identified 6-month consensus sequence viruses that had retained an ability to infect LCs similar to that of the donor-matched T/F virus. Moreover, some T/F viruses and 6-month consensus sequence viruses were unable to infect immature LCs. Further analyses indicated that T/F viruses are less sensitive to langerin-mediated restriction. These data suggest that T/F HIV-1 variants have the ability to infect immature LCs, which will facilitate transmission. |
| first_indexed | 2024-12-22T08:27:40Z |
| format | Article |
| id | doaj.art-7f2e847c87e44fb38eee994e4426b62e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T08:27:40Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7f2e847c87e44fb38eee994e4426b62e2022-12-21T18:32:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022665110.1371/journal.pone.0226651Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction.Nina HertoghsBernadien M NijmeijerNienke H van TeijlingenAngharad E Fenton-MayTanja M KapteinJohn L van HammeJohn C KappesNeeltje A KootstraBeatrice H HahnPersephone BorrowCarla M S RibeiroTeunis B H GeijtenbeekA single HIV-1 variant establishes infection of the host after sexual contact. Identifying the phenotypic characteristics of these Transmitted Founder (T/F) viruses is important to understand the restriction mechanisms during transmission. Langerhans cells (LCs) are the mucosal dendritic cell subset that has been shown to have a protective role in HIV-1 transmission. Immature LCs efficiently capture and degrade HIV-1 via langerin-mediated restriction. Here we have investigated the capacity of T/F HIV-1 strains to infect mucosal Langerhans cells (LCs). Notably, most T/F variants efficiently infected immature LCs derived from skin and vaginal tissue in contrast to chronic HIV-1 laboratory strains. Next we screened a panel of T/F viruses and their matched 6-month consensus sequence viruses. Interestingly most T/F variants infected immature LCs whereas donor-matched 6-month consensus sequence viruses had lost the ability to infect LCs. However, we also identified 6-month consensus sequence viruses that had retained an ability to infect LCs similar to that of the donor-matched T/F virus. Moreover, some T/F viruses and 6-month consensus sequence viruses were unable to infect immature LCs. Further analyses indicated that T/F viruses are less sensitive to langerin-mediated restriction. These data suggest that T/F HIV-1 variants have the ability to infect immature LCs, which will facilitate transmission.https://doi.org/10.1371/journal.pone.0226651 |
| spellingShingle | Nina Hertoghs Bernadien M Nijmeijer Nienke H van Teijlingen Angharad E Fenton-May Tanja M Kaptein John L van Hamme John C Kappes Neeltje A Kootstra Beatrice H Hahn Persephone Borrow Carla M S Ribeiro Teunis B H Geijtenbeek Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction. PLoS ONE |
| title | Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction. |
| title_full | Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction. |
| title_fullStr | Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction. |
| title_full_unstemmed | Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction. |
| title_short | Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction. |
| title_sort | sexually transmitted founder hiv 1 viruses are relatively resistant to langerhans cell mediated restriction |
| url | https://doi.org/10.1371/journal.pone.0226651 |
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