Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia
The GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and li...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada
2022-02-01
|
| Series: | Buletin Peternakan |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/buletinpeternakan/article/view/67188 |
| _version_ | 1828822213564825600 |
|---|---|
| author | Fatimah Az Zahra Chairunissa Sigit Bintara Tety Hartatik |
| author_facet | Fatimah Az Zahra Chairunissa Sigit Bintara Tety Hartatik |
| author_sort | Fatimah Az Zahra Chairunissa |
| collection | DOAJ |
| description | The GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and litter size in animals. This study aims to determine the kinship relationship of local goats compared to goats in Asia on prolific traits and to determine the restriction mapping of the GDF9 gene in goats based on the different SNP locations. The local goat comes from the Bligon goat, Kacang goat and Kejobong goats which is compared to the GenBank data (EF446168, EU883989 and KY780296). GDF9 sequences were analyzed using BioEdit and sequencing results to identify Single Nucleotide Polymorphism (SNP) and using NEBCutter V2 to determine the restriction enzyme which recognized the sequence around SNP. The result shows that three variations of SNP were found in exon 2 (g.3615T>C, g.3760T>C and g.3855A>C). Identification of SNP position found 1 SNP position identified by restriction enzyme at g.3855A> C. The identified restriction enzyme is HpaII and MspI. The results of this study are expected to provide genetic information that will be used for further research on the relationship between GDF9 gene polymorphisms to animal prolific. |
| first_indexed | 2024-12-12T13:09:47Z |
| format | Article |
| id | doaj.art-7f2e8d8aa29f4cc1a403041a09ec9cfe |
| institution | Directory Open Access Journal |
| issn | 0126-4400 2407-876X |
| language | English |
| last_indexed | 2024-12-12T13:09:47Z |
| publishDate | 2022-02-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Buletin Peternakan |
| spelling | doaj.art-7f2e8d8aa29f4cc1a403041a09ec9cfe2022-12-22T00:23:33ZengUniversitas Gadjah MadaBuletin Peternakan0126-44002407-876X2022-02-01461414510.21059/buletinpeternak.v46i1.6718831307Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in AsiaFatimah Az Zahra Chairunissa0Sigit Bintara1Tety Hartatik2Faculty Animal Science Universitas Gadjah MadaDepartment of Animal Breeding and Reproduction, Faculty of Animal Science Universitas Gadjah Mada, Yogyakarta, 55281Department of Animal Breeding and Reproduction, Faculty of Animal Science Universitas Gadjah Mada, Yogyakarta, 55281The GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and litter size in animals. This study aims to determine the kinship relationship of local goats compared to goats in Asia on prolific traits and to determine the restriction mapping of the GDF9 gene in goats based on the different SNP locations. The local goat comes from the Bligon goat, Kacang goat and Kejobong goats which is compared to the GenBank data (EF446168, EU883989 and KY780296). GDF9 sequences were analyzed using BioEdit and sequencing results to identify Single Nucleotide Polymorphism (SNP) and using NEBCutter V2 to determine the restriction enzyme which recognized the sequence around SNP. The result shows that three variations of SNP were found in exon 2 (g.3615T>C, g.3760T>C and g.3855A>C). Identification of SNP position found 1 SNP position identified by restriction enzyme at g.3855A> C. The identified restriction enzyme is HpaII and MspI. The results of this study are expected to provide genetic information that will be used for further research on the relationship between GDF9 gene polymorphisms to animal prolific.https://jurnal.ugm.ac.id/buletinpeternakan/article/view/67188gdf9goatsnpphylogeneticrestriction mapping |
| spellingShingle | Fatimah Az Zahra Chairunissa Sigit Bintara Tety Hartatik Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia Buletin Peternakan gdf9 goat snp phylogenetic restriction mapping |
| title | Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia |
| title_full | Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia |
| title_fullStr | Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia |
| title_full_unstemmed | Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia |
| title_short | Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia |
| title_sort | single nucleotide polymorphism of partial gdf9 gene in three local goat of indonesia compare with several goat in asia |
| topic | gdf9 goat snp phylogenetic restriction mapping |
| url | https://jurnal.ugm.ac.id/buletinpeternakan/article/view/67188 |
| work_keys_str_mv | AT fatimahazzahrachairunissa singlenucleotidepolymorphismofpartialgdf9geneinthreelocalgoatofindonesiacomparewithseveralgoatinasia AT sigitbintara singlenucleotidepolymorphismofpartialgdf9geneinthreelocalgoatofindonesiacomparewithseveralgoatinasia AT tetyhartatik singlenucleotidepolymorphismofpartialgdf9geneinthreelocalgoatofindonesiacomparewithseveralgoatinasia |