Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose diet
Background: Consumed fructose enters enterocytes of the intestinal epithelial apical membrane mediated by glucose transporter 5 (GLUT5). Aim: To determine the effects of Lombok Island's local Moringa leaf powder on reducing liver fructose levels and GLUT5 expression in the small intestine...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Tripoli University
2023-05-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | https://www.openveterinaryjournal.com/?mno=143461 |
| _version_ | 1797794229757411328 |
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| author | Aladhiana Cahyaningrum Masruroh Rahayu Achmad Rudijanto Dian Handayani |
| author_facet | Aladhiana Cahyaningrum Masruroh Rahayu Achmad Rudijanto Dian Handayani |
| author_sort | Aladhiana Cahyaningrum |
| collection | DOAJ |
| description | Background:
Consumed fructose enters enterocytes of the intestinal epithelial apical membrane mediated by glucose transporter 5 (GLUT5).
Aim:
To determine the effects of Lombok Island's local Moringa leaf powder on reducing liver fructose levels and GLUT5 expression in the small intestine of albino rats (Rattus novergicus) fed a high-fructose diet.
Methods:
Moringa leaf (Moringa oleifera) was obtained from Lombok Island, Indonesia. Subsequently, 30 male albino rats (Rattus novergicus) were used, divided into the normal group (NG), treatment group 1 (T1G), treatment group 2 (T2G), Quercetin group (QG), Moringa group (MG). Quercetin and moringa leaf powder (Moringa oleifera) was administered at 50 mg/kgbw and 500 mg/kgbw for 28 days. Enzyme-linked immunosorbent assay (Elisa) method used to examine liver fructose levels. The observation of GLUT5 expression in the small intestine was performed by the Immunofluorescence method.
Results:
The Anova test prove that there are significant differences (p < 0.005) in liver fructose levels in all groups. Further post hoc tests show no significant difference (p>0.005) in liver fructose levels in rats fed a high fructose diet in T1G and T2G with QG and MG rats. However, Moringa leaf powder freduces liver fructose levels by 32.1% and 17.2% in T1G and T2G rats.
The Anova test showed a significant difference (p < 0.005) in the expression of GLUT5 in all groups. Further post hoc tests showed a significant difference (p < 0.005) in the expression of GLUT5 in the duodenum, jejunum, and ileum between the normal group and the T1G rats. Meanwhile, in T2G rats, significant differences were only found in the jejunum. Moringa leaf powder reduces GLUT5 expression in T1G rats by 44.5%, 59.5%, and 57.2% in the duodenum, jejunum, and ileum, whereas in T2G rats is by 33.5%, 50.2%, and 48.1%, respectively.
Conclusion:
The administration of local moringa (Moringa oleifera) leaf powder in Lombok Island had an effect on reducing GLUT5 expression in the small intestine, however, did not in liver fructose levels of albino rats (Rattus novergicus) fed a high-fructose diet. [Open Vet J 2023; 13(5.000): 532-540] |
| first_indexed | 2024-03-13T02:59:14Z |
| format | Article |
| id | doaj.art-7f2ec56beb89436bb847c2241326388d |
| institution | Directory Open Access Journal |
| issn | 2226-4485 |
| language | English |
| last_indexed | 2024-03-13T02:59:14Z |
| publishDate | 2023-05-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-7f2ec56beb89436bb847c2241326388d2023-06-27T16:19:47ZengTripoli UniversityOpen Veterinary Journal2226-44852023-05-0113553254010.5455/OVJ.2023.v13.i5.5143461Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose dietAladhiana Cahyaningrum0Masruroh Rahayu1Achmad Rudijanto2Dian Handayani3Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Brawijaya Jl. Veteran, Malang 65145, East Java, Indonesia Endocrine and Metabolic Division, Internal Medicine, Dr Saiful Anwar Hospital, Malang. Jl. Jaksa Agung Suprapto No.2 Klojen District, Malang City, East Java. 65112, Indonesia Faculty of Medicine, Universitas Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia Faculty of Health Sciences,Universitas Brawijaya, Jl. Puncak Dieng, Kunci, Kalisongo, DauDistrict, Malang 65151 East Java, Indonesia.Background: Consumed fructose enters enterocytes of the intestinal epithelial apical membrane mediated by glucose transporter 5 (GLUT5). Aim: To determine the effects of Lombok Island's local Moringa leaf powder on reducing liver fructose levels and GLUT5 expression in the small intestine of albino rats (Rattus novergicus) fed a high-fructose diet. Methods: Moringa leaf (Moringa oleifera) was obtained from Lombok Island, Indonesia. Subsequently, 30 male albino rats (Rattus novergicus) were used, divided into the normal group (NG), treatment group 1 (T1G), treatment group 2 (T2G), Quercetin group (QG), Moringa group (MG). Quercetin and moringa leaf powder (Moringa oleifera) was administered at 50 mg/kgbw and 500 mg/kgbw for 28 days. Enzyme-linked immunosorbent assay (Elisa) method used to examine liver fructose levels. The observation of GLUT5 expression in the small intestine was performed by the Immunofluorescence method. Results: The Anova test prove that there are significant differences (p < 0.005) in liver fructose levels in all groups. Further post hoc tests show no significant difference (p>0.005) in liver fructose levels in rats fed a high fructose diet in T1G and T2G with QG and MG rats. However, Moringa leaf powder freduces liver fructose levels by 32.1% and 17.2% in T1G and T2G rats. The Anova test showed a significant difference (p < 0.005) in the expression of GLUT5 in all groups. Further post hoc tests showed a significant difference (p < 0.005) in the expression of GLUT5 in the duodenum, jejunum, and ileum between the normal group and the T1G rats. Meanwhile, in T2G rats, significant differences were only found in the jejunum. Moringa leaf powder reduces GLUT5 expression in T1G rats by 44.5%, 59.5%, and 57.2% in the duodenum, jejunum, and ileum, whereas in T2G rats is by 33.5%, 50.2%, and 48.1%, respectively. Conclusion: The administration of local moringa (Moringa oleifera) leaf powder in Lombok Island had an effect on reducing GLUT5 expression in the small intestine, however, did not in liver fructose levels of albino rats (Rattus novergicus) fed a high-fructose diet. [Open Vet J 2023; 13(5.000): 532-540]https://www.openveterinaryjournal.com/?mno=143461fructose levelsglut5 expressionhigh fructose dietlocal moringa leaf powderquercetin |
| spellingShingle | Aladhiana Cahyaningrum Masruroh Rahayu Achmad Rudijanto Dian Handayani Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose diet Open Veterinary Journal fructose levels glut5 expression high fructose diet local moringa leaf powder quercetin |
| title | Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose diet |
| title_full | Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose diet |
| title_fullStr | Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose diet |
| title_full_unstemmed | Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose diet |
| title_short | Effects of Lombok Island's local moringa (Moringa oleifera) leaf powder on the decrease of liver fructose levels and GLUT5 expression in albino rats (Rattus novergicus) fed a high fructose diet |
| title_sort | effects of lombok island apos s local moringa moringa oleifera leaf powder on the decrease of liver fructose levels and glut5 expression in albino rats rattus novergicus fed a high fructose diet |
| topic | fructose levels glut5 expression high fructose diet local moringa leaf powder quercetin |
| url | https://www.openveterinaryjournal.com/?mno=143461 |
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