| Summary: | The present study investigated the antioxidant enzyme activities (AEA) of ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX), and superoxide dismutase (SOD) as biomarkers of Cu and Pb stress by using <i>Centella asiatica</i> grown in an experimental hydroponic condition. The results showed (i) higher accumulations of Cu and Pb in the roots of <i>C. asiatica</i> than those in the leaves, (ii) synergistic effects of Cu and Pb stress at higher metal-level exposures, and (iii) Cu and Pb stress triggered the increment of APX, CAT, GPX, and SOD levels in both the leaves and roots of <i>C. asiatica</i>. The increment of four AEA indicated that <i>C. asiatica</i> underwent oxidative stress caused by the production of reactive oxygen species when the plant was exposed to Cu and Pb. In order to prevent damages caused by Cu and Pb stress, the AEA system was heightened in <i>C. asiatica</i>, in which APX, CAT, GPX, and SOD can be used as biomarkers of Pb and Cu stress in the plant.
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