Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i>
The present study investigated the antioxidant enzyme activities (AEA) of ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX), and superoxide dismutase (SOD) as biomarkers of Cu and Pb stress by using <i>Centella asiatica</i> grown in an experimental hydroponic conditio...
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/2673-7140/1/4/18 |
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| author | Chee Kong Yap Wen Siang Tan Koe Wei Wong Ghim Hock Ong Wan Hee Cheng Rosimah Nulit Mohd. Hafiz Ibrahim Weiyun Chew Franklin Berandah Edward Hideo Okamura Khalid Awadh Al-Mutairi Salman Abdo Al-Shami Moslem Sharifinia Muskhazli Mustafa Wah June Leong Chen Feng You |
| author_facet | Chee Kong Yap Wen Siang Tan Koe Wei Wong Ghim Hock Ong Wan Hee Cheng Rosimah Nulit Mohd. Hafiz Ibrahim Weiyun Chew Franklin Berandah Edward Hideo Okamura Khalid Awadh Al-Mutairi Salman Abdo Al-Shami Moslem Sharifinia Muskhazli Mustafa Wah June Leong Chen Feng You |
| author_sort | Chee Kong Yap |
| collection | DOAJ |
| description | The present study investigated the antioxidant enzyme activities (AEA) of ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX), and superoxide dismutase (SOD) as biomarkers of Cu and Pb stress by using <i>Centella asiatica</i> grown in an experimental hydroponic condition. The results showed (i) higher accumulations of Cu and Pb in the roots of <i>C. asiatica</i> than those in the leaves, (ii) synergistic effects of Cu and Pb stress at higher metal-level exposures, and (iii) Cu and Pb stress triggered the increment of APX, CAT, GPX, and SOD levels in both the leaves and roots of <i>C. asiatica</i>. The increment of four AEA indicated that <i>C. asiatica</i> underwent oxidative stress caused by the production of reactive oxygen species when the plant was exposed to Cu and Pb. In order to prevent damages caused by Cu and Pb stress, the AEA system was heightened in <i>C. asiatica</i>, in which APX, CAT, GPX, and SOD can be used as biomarkers of Pb and Cu stress in the plant. |
| first_indexed | 2024-03-10T03:04:54Z |
| format | Article |
| id | doaj.art-7f3061a564d245fea5f6c30e32d6a681 |
| institution | Directory Open Access Journal |
| issn | 2673-7140 |
| language | English |
| last_indexed | 2024-03-10T03:04:54Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Stresses |
| spelling | doaj.art-7f3061a564d245fea5f6c30e32d6a6812023-11-23T10:35:13ZengMDPI AGStresses2673-71402021-11-011425326510.3390/stresses1040018Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i>Chee Kong Yap0Wen Siang Tan1Koe Wei Wong2Ghim Hock Ong3Wan Hee Cheng4Rosimah Nulit5Mohd. Hafiz Ibrahim6Weiyun Chew7Franklin Berandah Edward8Hideo Okamura9Khalid Awadh Al-Mutairi10Salman Abdo Al-Shami11Moslem Sharifinia12Muskhazli Mustafa13Wah June Leong14Chen Feng You15Department of Biology, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaLaboratory of Vaccines and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaDepartment of Biology, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaFaculty of Science, Technology, Engineering and Mathematics, INTI International University, Nilai 71800, Negeri Sembilan, MalaysiaFaculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Nilai 71800, Negeri Sembilan, MalaysiaDepartment of Biology, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaDepartment of Biology, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaCentre for Pre-University Study, Level, 6, Unity Building, MAHSA University, Bandar Saujana Putra, Jenjarom 42610, Selangor, MalaysiaNatural Resources and Environment Board, Petra Jaya, Kuching 93050, Sarawak, MalaysiaGraduate School of Maritime Sciences, Faculty of Maritime Sciences, Kobe University, Kobe 658-0022, JapanDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk P.O. Box 741, Saudi ArabiaIndian River Research and Education Center, IFAS, University of Florida, Fort Pierce, FL 34945, USAShrimp Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr 7516989177, IranDepartment of Biology, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaDepartment of Mathematics and Statistics, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaDepartment of Earth Sciences, National Cheng-Kung University, No 1, University Road, Tainan City 701, TaiwanThe present study investigated the antioxidant enzyme activities (AEA) of ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX), and superoxide dismutase (SOD) as biomarkers of Cu and Pb stress by using <i>Centella asiatica</i> grown in an experimental hydroponic condition. The results showed (i) higher accumulations of Cu and Pb in the roots of <i>C. asiatica</i> than those in the leaves, (ii) synergistic effects of Cu and Pb stress at higher metal-level exposures, and (iii) Cu and Pb stress triggered the increment of APX, CAT, GPX, and SOD levels in both the leaves and roots of <i>C. asiatica</i>. The increment of four AEA indicated that <i>C. asiatica</i> underwent oxidative stress caused by the production of reactive oxygen species when the plant was exposed to Cu and Pb. In order to prevent damages caused by Cu and Pb stress, the AEA system was heightened in <i>C. asiatica</i>, in which APX, CAT, GPX, and SOD can be used as biomarkers of Pb and Cu stress in the plant.https://www.mdpi.com/2673-7140/1/4/18PbCu<i>Centella asiatica</i>antioxidant enzymes |
| spellingShingle | Chee Kong Yap Wen Siang Tan Koe Wei Wong Ghim Hock Ong Wan Hee Cheng Rosimah Nulit Mohd. Hafiz Ibrahim Weiyun Chew Franklin Berandah Edward Hideo Okamura Khalid Awadh Al-Mutairi Salman Abdo Al-Shami Moslem Sharifinia Muskhazli Mustafa Wah June Leong Chen Feng You Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i> Stresses Pb Cu <i>Centella asiatica</i> antioxidant enzymes |
| title | Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i> |
| title_full | Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i> |
| title_fullStr | Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i> |
| title_full_unstemmed | Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i> |
| title_short | Antioxidant Enzyme Activities as Biomarkers of Cu and Pb Stress in <i>Centella asiatica</i> |
| title_sort | antioxidant enzyme activities as biomarkers of cu and pb stress in i centella asiatica i |
| topic | Pb Cu <i>Centella asiatica</i> antioxidant enzymes |
| url | https://www.mdpi.com/2673-7140/1/4/18 |
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