A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells
Abstract Background Stemazole is a novel small molecule that has been suggested to have the ability to protect multiple stem cells. The proliferation-promoting activity and promising neuroprotective effects of stemazole make it a prospective drug for neurodegenerative disease treatment. Methods Sinc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | Proteome Science |
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| Online Access: | https://doi.org/10.1186/s12953-020-00168-2 |
| _version_ | 1818594692108910592 |
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| author | Huajun Li Yubo Zhang Jing Zhang Chaoran Zhao Yizi Zhu Mei Han |
| author_facet | Huajun Li Yubo Zhang Jing Zhang Chaoran Zhao Yizi Zhu Mei Han |
| author_sort | Huajun Li |
| collection | DOAJ |
| description | Abstract Background Stemazole is a novel small molecule that has been suggested to have the ability to protect multiple stem cells. The proliferation-promoting activity and promising neuroprotective effects of stemazole make it a prospective drug for neurodegenerative disease treatment. Methods Since previous studies have shown that it protective effect in extreme conditions, to understand more aspects of stemazole, in this study, a systematic tandem mass tags (TMT)-labelled proteomics approach was used to address the whole proteome expression profile with or without stemazole in normal conditions instead of extreme conditions. Bioinformatics analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and protein-protein interaction (PPI) network analyses, were employed. Results The effect of stemazole on the expression profiles of neural stem cells was obtained. A total of 408 proteins with changes at the abundance level of two groups were identified: 178 proteins increase in abundance and 240 proteins decrease in abundance, respectively. Low abundance of some mitochondrial respiratory chain enzyme, overproduction of reactive oxygen species (ROS) and reduction of mitochondrial membrane potential may indicate stemazole has cytotoxicity. Conclusions It is the first proteomics research about stemazole, and the possible cytotoxicity of stemazole has been reported for the first time. The information about proteins that were affected by stemazole and more characteristics of stemazole will help obtain a complete picture of this small molecule drug. These findings provide a scientific basis for further stemazole treatment research. |
| first_indexed | 2024-12-16T11:04:09Z |
| format | Article |
| id | doaj.art-7f344856a6da49daa7206a7e87f71471 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-16T11:04:09Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-7f344856a6da49daa7206a7e87f714712022-12-21T22:33:54ZengBMCProteome Science1477-59562020-12-0118111210.1186/s12953-020-00168-2A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cellsHuajun Li0Yubo Zhang1Jing Zhang2Chaoran Zhao3Yizi Zhu4Mei Han5Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal UniversityKey Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal UniversityKey Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal UniversityKey Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal UniversityKey Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal UniversityKey Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal UniversityAbstract Background Stemazole is a novel small molecule that has been suggested to have the ability to protect multiple stem cells. The proliferation-promoting activity and promising neuroprotective effects of stemazole make it a prospective drug for neurodegenerative disease treatment. Methods Since previous studies have shown that it protective effect in extreme conditions, to understand more aspects of stemazole, in this study, a systematic tandem mass tags (TMT)-labelled proteomics approach was used to address the whole proteome expression profile with or without stemazole in normal conditions instead of extreme conditions. Bioinformatics analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and protein-protein interaction (PPI) network analyses, were employed. Results The effect of stemazole on the expression profiles of neural stem cells was obtained. A total of 408 proteins with changes at the abundance level of two groups were identified: 178 proteins increase in abundance and 240 proteins decrease in abundance, respectively. Low abundance of some mitochondrial respiratory chain enzyme, overproduction of reactive oxygen species (ROS) and reduction of mitochondrial membrane potential may indicate stemazole has cytotoxicity. Conclusions It is the first proteomics research about stemazole, and the possible cytotoxicity of stemazole has been reported for the first time. The information about proteins that were affected by stemazole and more characteristics of stemazole will help obtain a complete picture of this small molecule drug. These findings provide a scientific basis for further stemazole treatment research.https://doi.org/10.1186/s12953-020-00168-2StemazoleSmall moleculeNeural stem cellsProteomicsProtein expression profiling |
| spellingShingle | Huajun Li Yubo Zhang Jing Zhang Chaoran Zhao Yizi Zhu Mei Han A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells Proteome Science Stemazole Small molecule Neural stem cells Proteomics Protein expression profiling |
| title | A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells |
| title_full | A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells |
| title_fullStr | A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells |
| title_full_unstemmed | A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells |
| title_short | A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells |
| title_sort | quantitative proteomics analysis for small molecule stemazole s effect on human neural stem cells |
| topic | Stemazole Small molecule Neural stem cells Proteomics Protein expression profiling |
| url | https://doi.org/10.1186/s12953-020-00168-2 |
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