A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots
Glucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. Herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and CdTe/thioglycolic acid (TGA) quantum dots (QDs) on a thin layer chromatograph...
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| Format: | Article |
| Language: | English |
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Iranian Chemical Society
2014-12-01
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| Series: | Analytical and Bioanalytical Chemistry Research |
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| Online Access: | http://www.analchemres.org/article_7315_93a545f2cae1055282d1846a1d75c527.pdf |
| _version_ | 1818426836040810496 |
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| author | Javad Tashkhourian Morteza Akhond S. Hooshmand T. Khosousi B. Hemmateenejad |
| author_facet | Javad Tashkhourian Morteza Akhond S. Hooshmand T. Khosousi B. Hemmateenejad |
| author_sort | Javad Tashkhourian |
| collection | DOAJ |
| description | Glucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. Herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and CdTe/thioglycolic acid (TGA) quantum dots (QDs) on a thin layer chromatography (TLC) plate has been described. The detection was based on the combination of the glucose enzymatic reaction and the quenching effect of H2O2 on the CdTe/TGA quantum dots photoluminescence. This QDs-based assay exhibits several advantages. Enzyme immobilization and QDs modification process are not required and the high stability of the QDs towards photobleaching is beneficial to this sensing system. The proposed method is linear in concentration range of 1.00 × 10-1-3.00 × 10-5 M of glucose and has a detection limit of 1.25 × 10-8 M. The results of real sample analysis show that the glucose oxidase CdTe/TGA QDs system would be a promising glucose-biosensing system. |
| first_indexed | 2024-12-14T14:36:09Z |
| format | Article |
| id | doaj.art-7f3685def3e44dbca7e3499b54a2e681 |
| institution | Directory Open Access Journal |
| issn | 2383-093X 2383-093X |
| language | English |
| last_indexed | 2024-12-14T14:36:09Z |
| publishDate | 2014-12-01 |
| publisher | Iranian Chemical Society |
| record_format | Article |
| series | Analytical and Bioanalytical Chemistry Research |
| spelling | doaj.art-7f3685def3e44dbca7e3499b54a2e6812022-12-21T22:57:37ZengIranian Chemical SocietyAnalytical and Bioanalytical Chemistry Research2383-093X2383-093X2014-12-011211712710.22036/abcr.2014.73157315A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum DotsJavad Tashkhourian0Morteza Akhond1S. Hooshmand2T. Khosousi3B. Hemmateenejad4Chemistry Department, Shiraz UniversityChemistry Department, Shiraz UniversityChemistry Department, Shiraz UniversityChemistry Department, Shiraz UniversityChemistry Department, Shiraz UniversityGlucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. Herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and CdTe/thioglycolic acid (TGA) quantum dots (QDs) on a thin layer chromatography (TLC) plate has been described. The detection was based on the combination of the glucose enzymatic reaction and the quenching effect of H2O2 on the CdTe/TGA quantum dots photoluminescence. This QDs-based assay exhibits several advantages. Enzyme immobilization and QDs modification process are not required and the high stability of the QDs towards photobleaching is beneficial to this sensing system. The proposed method is linear in concentration range of 1.00 × 10-1-3.00 × 10-5 M of glucose and has a detection limit of 1.25 × 10-8 M. The results of real sample analysis show that the glucose oxidase CdTe/TGA QDs system would be a promising glucose-biosensing system.http://www.analchemres.org/article_7315_93a545f2cae1055282d1846a1d75c527.pdfImage analysisCdTe/TGA Quantum dotsGlucoseGlucose oxidase |
| spellingShingle | Javad Tashkhourian Morteza Akhond S. Hooshmand T. Khosousi B. Hemmateenejad A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots Analytical and Bioanalytical Chemistry Research Image analysis CdTe/TGA Quantum dots Glucose Glucose oxidase |
| title | A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots |
| title_full | A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots |
| title_fullStr | A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots |
| title_full_unstemmed | A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots |
| title_short | A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots |
| title_sort | simple image analysis method for determination of glucose by using glucose oxidase cdte tga quantum dots |
| topic | Image analysis CdTe/TGA Quantum dots Glucose Glucose oxidase |
| url | http://www.analchemres.org/article_7315_93a545f2cae1055282d1846a1d75c527.pdf |
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