Nanopore analysis of salvianolic acids in herbal medicines
Abstract Natural herbs, which contain pharmacologically active compounds, have been used historically as medicines. Conventionally, the analysis of chemical components in herbal medicines requires time-consuming sample separation and state-of-the-art analytical instruments. Nanopore, a versatile sin...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-45543-1 |
| _version_ | 1797273961184100352 |
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| author | Pingping Fan Shanyu Zhang Yuqin Wang Tian Li Hanhan Zhang Panke Zhang Shuo Huang |
| author_facet | Pingping Fan Shanyu Zhang Yuqin Wang Tian Li Hanhan Zhang Panke Zhang Shuo Huang |
| author_sort | Pingping Fan |
| collection | DOAJ |
| description | Abstract Natural herbs, which contain pharmacologically active compounds, have been used historically as medicines. Conventionally, the analysis of chemical components in herbal medicines requires time-consuming sample separation and state-of-the-art analytical instruments. Nanopore, a versatile single molecule sensor, might be suitable to identify bioactive compounds in natural herbs. Here, a phenylboronic acid appended Mycobacterium smegmatis porin A (MspA) nanopore is used as a sensor for herbal medicines. A variety of bioactive compounds based on salvianolic acids, including caffeic acid, protocatechuic acid, protocatechualdehyde, salvianic acid A, rosmarinic acid, lithospermic acid, salvianolic acid A and salvianolic acid B are identified. Using a custom machine learning algorithm, analyte identification is performed with an accuracy of 99.0%. This sensing principle is further used with natural herbs such as Salvia miltiorrhiza, Rosemary and Prunella vulgaris. No complex sample separation or purification is required and the sensing device is highly portable. |
| first_indexed | 2024-03-07T14:51:36Z |
| format | Article |
| id | doaj.art-7f380fafcd0041129841b4ffab2c4d6e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-07T14:51:36Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-7f380fafcd0041129841b4ffab2c4d6e2024-03-05T19:39:39ZengNature PortfolioNature Communications2041-17232024-03-0115111210.1038/s41467-024-45543-1Nanopore analysis of salvianolic acids in herbal medicinesPingping Fan0Shanyu Zhang1Yuqin Wang2Tian Li3Hanhan Zhang4Panke Zhang5Shuo Huang6State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing UniversityState Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing UniversityAbstract Natural herbs, which contain pharmacologically active compounds, have been used historically as medicines. Conventionally, the analysis of chemical components in herbal medicines requires time-consuming sample separation and state-of-the-art analytical instruments. Nanopore, a versatile single molecule sensor, might be suitable to identify bioactive compounds in natural herbs. Here, a phenylboronic acid appended Mycobacterium smegmatis porin A (MspA) nanopore is used as a sensor for herbal medicines. A variety of bioactive compounds based on salvianolic acids, including caffeic acid, protocatechuic acid, protocatechualdehyde, salvianic acid A, rosmarinic acid, lithospermic acid, salvianolic acid A and salvianolic acid B are identified. Using a custom machine learning algorithm, analyte identification is performed with an accuracy of 99.0%. This sensing principle is further used with natural herbs such as Salvia miltiorrhiza, Rosemary and Prunella vulgaris. No complex sample separation or purification is required and the sensing device is highly portable.https://doi.org/10.1038/s41467-024-45543-1 |
| spellingShingle | Pingping Fan Shanyu Zhang Yuqin Wang Tian Li Hanhan Zhang Panke Zhang Shuo Huang Nanopore analysis of salvianolic acids in herbal medicines Nature Communications |
| title | Nanopore analysis of salvianolic acids in herbal medicines |
| title_full | Nanopore analysis of salvianolic acids in herbal medicines |
| title_fullStr | Nanopore analysis of salvianolic acids in herbal medicines |
| title_full_unstemmed | Nanopore analysis of salvianolic acids in herbal medicines |
| title_short | Nanopore analysis of salvianolic acids in herbal medicines |
| title_sort | nanopore analysis of salvianolic acids in herbal medicines |
| url | https://doi.org/10.1038/s41467-024-45543-1 |
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