Monocyte biology conserved across species: Functional insights from cattle
Similar to human monocytes, bovine monocytes can be split into CD14highCD16- classical, CD14highCD16high intermediate and CD14-/dimCD16high nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, high...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.889175/full |
| _version_ | 1827993497420955648 |
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| author | Stephanie C. Talker Stephanie C. Talker G. Tuba Barut G. Tuba Barut Heidi E.L. Lischer Reto Rufener Lilly von Münchow Rémy Bruggmann Artur Summerfield Artur Summerfield |
| author_facet | Stephanie C. Talker Stephanie C. Talker G. Tuba Barut G. Tuba Barut Heidi E.L. Lischer Reto Rufener Lilly von Münchow Rémy Bruggmann Artur Summerfield Artur Summerfield |
| author_sort | Stephanie C. Talker |
| collection | DOAJ |
| description | Similar to human monocytes, bovine monocytes can be split into CD14highCD16- classical, CD14highCD16high intermediate and CD14-/dimCD16high nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both pronounced functional specializations and transcriptomic relatedness. Bulk gene transcription indicates pro-inflammatory and antibacterial roles of cM, while ncM and intM appear to be specialized in regulatory/anti-inflammatory functions and tissue repair, as well as antiviral responses and T-cell immunomodulation. Notably, intM stood out by high expression of several genes associated with antigen presentation. Anti-inflammatory and antiviral functions of ncM are further supported by dominant oxidative phosphorylation and selective strong responses to TLR7/8 ligands, respectively. Moreover, single-cell RNA-seq revealed previously unappreciated heterogeneity within cM and proposes intM as a transient differentiation intermediate between cM and ncM. |
| first_indexed | 2024-04-10T04:26:16Z |
| format | Article |
| id | doaj.art-7f3910f9bb254742bf6e108c4517f8f6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T04:26:16Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7f3910f9bb254742bf6e108c4517f8f62023-03-10T12:11:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.889175889175Monocyte biology conserved across species: Functional insights from cattleStephanie C. Talker0Stephanie C. Talker1G. Tuba Barut2G. Tuba Barut3Heidi E.L. Lischer4Reto Rufener5Lilly von Münchow6Rémy Bruggmann7Artur Summerfield8Artur Summerfield9Institute of Virology and Immunology, Bern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandInstitute of Virology and Immunology, Bern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandInterfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, SwitzerlandInstitute of Parasitology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandBucher Biotec AG, Basel, SwitzerlandInterfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, SwitzerlandInstitute of Virology and Immunology, Bern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandSimilar to human monocytes, bovine monocytes can be split into CD14highCD16- classical, CD14highCD16high intermediate and CD14-/dimCD16high nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both pronounced functional specializations and transcriptomic relatedness. Bulk gene transcription indicates pro-inflammatory and antibacterial roles of cM, while ncM and intM appear to be specialized in regulatory/anti-inflammatory functions and tissue repair, as well as antiviral responses and T-cell immunomodulation. Notably, intM stood out by high expression of several genes associated with antigen presentation. Anti-inflammatory and antiviral functions of ncM are further supported by dominant oxidative phosphorylation and selective strong responses to TLR7/8 ligands, respectively. Moreover, single-cell RNA-seq revealed previously unappreciated heterogeneity within cM and proposes intM as a transient differentiation intermediate between cM and ncM.https://www.frontiersin.org/articles/10.3389/fimmu.2022.889175/fullbovine monocyte subsetsclassical monocytesintermediate monocytesnonclassical monocytestranscriptomesingle-cell RNA sequencing (scRNA-seq) |
| spellingShingle | Stephanie C. Talker Stephanie C. Talker G. Tuba Barut G. Tuba Barut Heidi E.L. Lischer Reto Rufener Lilly von Münchow Rémy Bruggmann Artur Summerfield Artur Summerfield Monocyte biology conserved across species: Functional insights from cattle Frontiers in Immunology bovine monocyte subsets classical monocytes intermediate monocytes nonclassical monocytes transcriptome single-cell RNA sequencing (scRNA-seq) |
| title | Monocyte biology conserved across species: Functional insights from cattle |
| title_full | Monocyte biology conserved across species: Functional insights from cattle |
| title_fullStr | Monocyte biology conserved across species: Functional insights from cattle |
| title_full_unstemmed | Monocyte biology conserved across species: Functional insights from cattle |
| title_short | Monocyte biology conserved across species: Functional insights from cattle |
| title_sort | monocyte biology conserved across species functional insights from cattle |
| topic | bovine monocyte subsets classical monocytes intermediate monocytes nonclassical monocytes transcriptome single-cell RNA sequencing (scRNA-seq) |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.889175/full |
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