Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes
Peroxidized phospholipid-mediated cytotoxicity is involved in the pathophysiology of diseases [i.e., an abnormal increase of phosphatidylcholine hydroperoxide (PCOOH) in plasma of type 2 diabetic patients]. The PCOOH accumulation may relate to Amadori-glycated phosphatidylethanolamine (Amadori-PE; d...
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| Format: | Article |
| Language: | English |
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Elsevier
2005-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752032890X |
| _version_ | 1818601193479340032 |
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| author | Kiyotaka Nakagawa Jeong-Ho Oak Ohki Higuchi Tsuyoshi Tsuzuki Shinichi Oikawa Haruhisa Otani Masatoshi Mune Hua Cai Teruo Miyazawa |
| author_facet | Kiyotaka Nakagawa Jeong-Ho Oak Ohki Higuchi Tsuyoshi Tsuzuki Shinichi Oikawa Haruhisa Otani Masatoshi Mune Hua Cai Teruo Miyazawa |
| author_sort | Kiyotaka Nakagawa |
| collection | DOAJ |
| description | Peroxidized phospholipid-mediated cytotoxicity is involved in the pathophysiology of diseases [i.e., an abnormal increase of phosphatidylcholine hydroperoxide (PCOOH) in plasma of type 2 diabetic patients]. The PCOOH accumulation may relate to Amadori-glycated phosphatidylethanolamine (Amadori-PE; deoxy-d-fructosyl phosphatidylethanolamine), because Amadori-PE causes oxidative stress. However, the occurrence of lipid glycation products, including Amadori-PE, in vivo is still unclear. Consequently, we developed an analysis method of Amadori-PE using a quadrupole/linear ion-trap mass spectrometer, the Applied Biosystems QTRAP. In positive ion mode, collision-induced dissociation of Amadori-PE produced a well-characterized diglyceride ion ([M+H−303]+) permitting neutral loss scanning and multiple reaction monitoring (MRM). When lipid extract from diabetic plasma was infused directly into the QTRAP, Amadori-PE molecular species could be screened out by neutral loss scanning. Interfacing liquid chromatography with QTRAP mass spectrometry enabled the separation and determination of predominant plasma Amadori-PE species with sensitivity of ∼0.1 pmol/injection in MRM. The plasma Amadori-PE level was 0.08 mol% of total PE in healthy subjects and 0.15–0.29 mol% in diabetic patients. Furthermore, plasma Amadori-PE levels were positively correlated with PCOOH (a maker for oxidative stress).These results show the involvement between lipid glycation and lipid peroxidation in diabetes pathogenesis. |
| first_indexed | 2024-12-16T12:47:29Z |
| format | Article |
| id | doaj.art-7f3a77deb0fe4ac08ebf4babcbf9195f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T12:47:29Z |
| publishDate | 2005-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7f3a77deb0fe4ac08ebf4babcbf9195f2022-12-21T22:31:15ZengElsevierJournal of Lipid Research0022-22752005-11-01461125142524Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetesKiyotaka Nakagawa0Jeong-Ho Oak1Ohki Higuchi2Tsuyoshi Tsuzuki3Shinichi Oikawa4Haruhisa Otani5Masatoshi Mune6Hua Cai7Teruo Miyazawa8Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanFood and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanFood and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanFood and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanDepartment of Medicine, Nippon Medical School, Tokyo 113-8603, JapanDepartment of Internal Medicine, Wakayama Medical College, Wakayama 641-8509, JapanDepartment of Internal Medicine, Wakayama Medical College, Wakayama 641-8509, JapanSection of Cardiology, Department of Medicine, Division of Biological Sciences and Pritzker School of Medicine, University of Chicago, Chicago, IL 60637To whom correspondence should be addressed.; Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanPeroxidized phospholipid-mediated cytotoxicity is involved in the pathophysiology of diseases [i.e., an abnormal increase of phosphatidylcholine hydroperoxide (PCOOH) in plasma of type 2 diabetic patients]. The PCOOH accumulation may relate to Amadori-glycated phosphatidylethanolamine (Amadori-PE; deoxy-d-fructosyl phosphatidylethanolamine), because Amadori-PE causes oxidative stress. However, the occurrence of lipid glycation products, including Amadori-PE, in vivo is still unclear. Consequently, we developed an analysis method of Amadori-PE using a quadrupole/linear ion-trap mass spectrometer, the Applied Biosystems QTRAP. In positive ion mode, collision-induced dissociation of Amadori-PE produced a well-characterized diglyceride ion ([M+H−303]+) permitting neutral loss scanning and multiple reaction monitoring (MRM). When lipid extract from diabetic plasma was infused directly into the QTRAP, Amadori-PE molecular species could be screened out by neutral loss scanning. Interfacing liquid chromatography with QTRAP mass spectrometry enabled the separation and determination of predominant plasma Amadori-PE species with sensitivity of ∼0.1 pmol/injection in MRM. The plasma Amadori-PE level was 0.08 mol% of total PE in healthy subjects and 0.15–0.29 mol% in diabetic patients. Furthermore, plasma Amadori-PE levels were positively correlated with PCOOH (a maker for oxidative stress).These results show the involvement between lipid glycation and lipid peroxidation in diabetes pathogenesis.http://www.sciencedirect.com/science/article/pii/S002222752032890XglycationQTRAP mass spectrometerlipid peroxidation |
| spellingShingle | Kiyotaka Nakagawa Jeong-Ho Oak Ohki Higuchi Tsuyoshi Tsuzuki Shinichi Oikawa Haruhisa Otani Masatoshi Mune Hua Cai Teruo Miyazawa Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes Journal of Lipid Research glycation QTRAP mass spectrometer lipid peroxidation |
| title | Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes |
| title_full | Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes |
| title_fullStr | Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes |
| title_full_unstemmed | Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes |
| title_short | Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes |
| title_sort | ion trap tandem mass spectrometric analysis of amadori glycated phosphatidylethanolamine in human plasma with or without diabetes |
| topic | glycation QTRAP mass spectrometer lipid peroxidation |
| url | http://www.sciencedirect.com/science/article/pii/S002222752032890X |
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