Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis
Sprouting angiogenesis is the common response of live tissues to physiological and pathological angiogenic stimuli. Its accurate evaluation is of utmost importance for basic research and practical medicine and pharmacology and requires adequate experimental models. A variety of assays for angiogenes...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/11/1/18 |
| _version_ | 1797495748455038976 |
|---|---|
| author | Igor V. Vakhrushev Elizaveta K. Nezhurina Pavel A. Karalkin Anastasia V. Tsvetkova Nataliya S. Sergeeva Alexander G. Majouga Konstantin N. Yarygin |
| author_facet | Igor V. Vakhrushev Elizaveta K. Nezhurina Pavel A. Karalkin Anastasia V. Tsvetkova Nataliya S. Sergeeva Alexander G. Majouga Konstantin N. Yarygin |
| author_sort | Igor V. Vakhrushev |
| collection | DOAJ |
| description | Sprouting angiogenesis is the common response of live tissues to physiological and pathological angiogenic stimuli. Its accurate evaluation is of utmost importance for basic research and practical medicine and pharmacology and requires adequate experimental models. A variety of assays for angiogenesis were developed, none of them perfect. In vitro approaches are generally less physiologically relevant due to the omission of essential components regulating the process. However, only in vitro models can be entirely non-xenogeneic. The limitations of the in vitro angiogenesis assays can be partially overcome using 3D models mimicking tissue O<sub>2</sub> and nutrient gradients, the influence of the extracellular matrix (ECM), and enabling cell-cell interactions. Here we present a review of the existing models of sprouting angiogenesis that are based on the use of endothelial cells (ECs) co-cultured with perivascular or other stromal cells. This approach provides an excellent in vitro platform for further decoding of the cellular and molecular mechanisms of sprouting angiogenesis under conditions close to the in vivo conditions, as well as for preclinical drug testing and preclinical research in tissue engineering and regenerative medicine. |
| first_indexed | 2024-03-10T01:54:03Z |
| format | Article |
| id | doaj.art-7f3d3ba9588b4d2fb1984eaa13d60f62 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T01:54:03Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-7f3d3ba9588b4d2fb1984eaa13d60f622023-11-23T12:59:48ZengMDPI AGBiology2079-77372021-12-011111810.3390/biology11010018Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting AngiogenesisIgor V. Vakhrushev0Elizaveta K. Nezhurina1Pavel A. Karalkin2Anastasia V. Tsvetkova3Nataliya S. Sergeeva4Alexander G. Majouga5Konstantin N. Yarygin6Laboratory of Cell Biology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaP.A. Hertsen Moscow Oncology Research Center, National Medical Research Radiological Center, 125284 Moscow, RussiaInstitute for Cluster Oncology, Sechenov University, 119435 Moscow, RussiaLaboratory of Cell Biology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaDepartment of Biology, Pirogov Russian National Research Medical University, 117997 Moscow, RussiaFaculty of Chemical and Pharmaceutical Technologies and Biomedical Products, D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, RussiaLaboratory of Cell Biology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaSprouting angiogenesis is the common response of live tissues to physiological and pathological angiogenic stimuli. Its accurate evaluation is of utmost importance for basic research and practical medicine and pharmacology and requires adequate experimental models. A variety of assays for angiogenesis were developed, none of them perfect. In vitro approaches are generally less physiologically relevant due to the omission of essential components regulating the process. However, only in vitro models can be entirely non-xenogeneic. The limitations of the in vitro angiogenesis assays can be partially overcome using 3D models mimicking tissue O<sub>2</sub> and nutrient gradients, the influence of the extracellular matrix (ECM), and enabling cell-cell interactions. Here we present a review of the existing models of sprouting angiogenesis that are based on the use of endothelial cells (ECs) co-cultured with perivascular or other stromal cells. This approach provides an excellent in vitro platform for further decoding of the cellular and molecular mechanisms of sprouting angiogenesis under conditions close to the in vivo conditions, as well as for preclinical drug testing and preclinical research in tissue engineering and regenerative medicine.https://www.mdpi.com/2079-7737/11/1/18tissue spheroidssprouting angiogenesisendothelial cellsperivascular cellsmesenchymal stem cellsin vitro angiogenesis models |
| spellingShingle | Igor V. Vakhrushev Elizaveta K. Nezhurina Pavel A. Karalkin Anastasia V. Tsvetkova Nataliya S. Sergeeva Alexander G. Majouga Konstantin N. Yarygin Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis Biology tissue spheroids sprouting angiogenesis endothelial cells perivascular cells mesenchymal stem cells in vitro angiogenesis models |
| title | Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis |
| title_full | Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis |
| title_fullStr | Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis |
| title_full_unstemmed | Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis |
| title_short | Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis |
| title_sort | heterotypic multicellular spheroids as experimental and preclinical models of sprouting angiogenesis |
| topic | tissue spheroids sprouting angiogenesis endothelial cells perivascular cells mesenchymal stem cells in vitro angiogenesis models |
| url | https://www.mdpi.com/2079-7737/11/1/18 |
| work_keys_str_mv | AT igorvvakhrushev heterotypicmulticellularspheroidsasexperimentalandpreclinicalmodelsofsproutingangiogenesis AT elizavetaknezhurina heterotypicmulticellularspheroidsasexperimentalandpreclinicalmodelsofsproutingangiogenesis AT pavelakaralkin heterotypicmulticellularspheroidsasexperimentalandpreclinicalmodelsofsproutingangiogenesis AT anastasiavtsvetkova heterotypicmulticellularspheroidsasexperimentalandpreclinicalmodelsofsproutingangiogenesis AT nataliyassergeeva heterotypicmulticellularspheroidsasexperimentalandpreclinicalmodelsofsproutingangiogenesis AT alexandergmajouga heterotypicmulticellularspheroidsasexperimentalandpreclinicalmodelsofsproutingangiogenesis AT konstantinnyarygin heterotypicmulticellularspheroidsasexperimentalandpreclinicalmodelsofsproutingangiogenesis |