Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress
Background The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable...
| Main Authors: | , , , , , |
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| Format: | Article |
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PeerJ Inc.
2018-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/5136.pdf |
| _version_ | 1797421754976567296 |
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| author | Matheus Marcon Ricieri Mocelin Adrieli Sachett Anna M. Siebel Ana P. Herrmann Angelo Piato |
| author_facet | Matheus Marcon Ricieri Mocelin Adrieli Sachett Anna M. Siebel Ana P. Herrmann Angelo Piato |
| author_sort | Matheus Marcon |
| collection | DOAJ |
| description | Background The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Methods Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Results Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days increased CAT activity. Discussion Our findings reinforce the idea that EE exerts neuromodulatory effects in the zebrafish brain. EE promoted positive effects as it helped maintain the redox homeostasis, which may reduce the susceptibility to stress and its oxidative impact. |
| first_indexed | 2024-03-09T07:22:00Z |
| format | Article |
| id | doaj.art-7f3f528217de4925ba46d7c347c1df3e |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:22:00Z |
| publishDate | 2018-07-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-7f3f528217de4925ba46d7c347c1df3e2023-12-03T07:14:46ZengPeerJ Inc.PeerJ2167-83592018-07-016e513610.7717/peerj.5136Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stressMatheus Marcon0Ricieri Mocelin1Adrieli Sachett2Anna M. Siebel3Ana P. Herrmann4Angelo Piato5Programa de Pós-graduação em Neurociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilPrograma de Pós-graduação em Neurociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilPrograma de Pós-graduação em Neurociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilPrograma de Pós-graduação em Ciências Ambientais, Universidade Comunitária da Região de Chapecó, Chapecó, SC, BrazilPrograma de Pós-graduação em Farmacologia e Terapêutica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilPrograma de Pós-graduação em Neurociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilBackground The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Methods Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Results Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days increased CAT activity. Discussion Our findings reinforce the idea that EE exerts neuromodulatory effects in the zebrafish brain. EE promoted positive effects as it helped maintain the redox homeostasis, which may reduce the susceptibility to stress and its oxidative impact.https://peerj.com/articles/5136.pdfEnvironmental enrichmentUnpredictable chronic stressOxidative stressZebrafish |
| spellingShingle | Matheus Marcon Ricieri Mocelin Adrieli Sachett Anna M. Siebel Ana P. Herrmann Angelo Piato Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress PeerJ Environmental enrichment Unpredictable chronic stress Oxidative stress Zebrafish |
| title | Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
| title_full | Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
| title_fullStr | Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
| title_full_unstemmed | Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
| title_short | Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
| title_sort | enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress |
| topic | Environmental enrichment Unpredictable chronic stress Oxidative stress Zebrafish |
| url | https://peerj.com/articles/5136.pdf |
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