Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies
The aim of this study was to determine the grain composition and (poly)phenolic profile of pollen from Abrantes (Portugal), as well as its antioxidative and antidiabetic properties, and abilities to protect human erythrocytes against induced hemoglobin oxidation, lipid peroxidation, and hemolysis. T...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/11/2804 |
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| author | Ana C. Gonçalves Radhia Aitfella Lahlou Gilberto Alves Cristina Garcia-Viguera Diego A. Moreno Luís R. Silva |
| author_facet | Ana C. Gonçalves Radhia Aitfella Lahlou Gilberto Alves Cristina Garcia-Viguera Diego A. Moreno Luís R. Silva |
| author_sort | Ana C. Gonçalves |
| collection | DOAJ |
| description | The aim of this study was to determine the grain composition and (poly)phenolic profile of pollen from Abrantes (Portugal), as well as its antioxidative and antidiabetic properties, and abilities to protect human erythrocytes against induced hemoglobin oxidation, lipid peroxidation, and hemolysis. The phytochemical profile of the Abrantes’ bee pollen revealed twenty phenolic compounds, identified by high-performance liquid chromatography with electrospray ionization mass spectrometry coupled with photodiode array detection. Among them, quercetin derivatives were the most abundant. Concerning the biological potential, the pollen extract showed notable capacity for 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, and superoxide radicals, as well as for inhibition of α-glucosidase action, and protection of human erythrocytes against oxidative damage. Non-cytotoxic effects regarding the NHDF normal cell line, human adenocarcinoma Caco-2, and human liver HepG2 cells were observed. The results obtained contributed to further research on modes of action related to oxidative damage and metabolic health problems, to generate deeper knowledge of potential health-promoting effects to develop novel pharmaceutical drugs, nutraceuticals, and dietary supplements. |
| first_indexed | 2024-03-10T05:29:37Z |
| format | Article |
| id | doaj.art-7f43a276a86c4d07a49fb8f02b75a27c |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T05:29:37Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-7f43a276a86c4d07a49fb8f02b75a27c2023-11-22T23:22:17ZengMDPI AGFoods2304-81582021-11-011011280410.3390/foods10112804Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model StudiesAna C. Gonçalves0Radhia Aitfella Lahlou1Gilberto Alves2Cristina Garcia-Viguera3Diego A. Moreno4Luís R. Silva5CICS–UBI–Health Sciences Research Centre, Department of Medical Science, University of Beira Interior, 6201-506 Covilhã, PortugalChemistry Department, University of Beira Interior, 6201-001 Covilhã, PortugalCICS–UBI–Health Sciences Research Centre, Department of Medical Science, University of Beira Interior, 6201-506 Covilhã, PortugalPhytochemistry and Healthy Foods Laboratory, Food Science and Technology Department, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Espinardo, Murcia, SpainPhytochemistry and Healthy Foods Laboratory, Food Science and Technology Department, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Espinardo, Murcia, SpainCICS–UBI–Health Sciences Research Centre, Department of Medical Science, University of Beira Interior, 6201-506 Covilhã, PortugalThe aim of this study was to determine the grain composition and (poly)phenolic profile of pollen from Abrantes (Portugal), as well as its antioxidative and antidiabetic properties, and abilities to protect human erythrocytes against induced hemoglobin oxidation, lipid peroxidation, and hemolysis. The phytochemical profile of the Abrantes’ bee pollen revealed twenty phenolic compounds, identified by high-performance liquid chromatography with electrospray ionization mass spectrometry coupled with photodiode array detection. Among them, quercetin derivatives were the most abundant. Concerning the biological potential, the pollen extract showed notable capacity for 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, and superoxide radicals, as well as for inhibition of α-glucosidase action, and protection of human erythrocytes against oxidative damage. Non-cytotoxic effects regarding the NHDF normal cell line, human adenocarcinoma Caco-2, and human liver HepG2 cells were observed. The results obtained contributed to further research on modes of action related to oxidative damage and metabolic health problems, to generate deeper knowledge of potential health-promoting effects to develop novel pharmaceutical drugs, nutraceuticals, and dietary supplements.https://www.mdpi.com/2304-8158/10/11/2804bee pollenphenolic characterizationbiological potentialfunctional foodantioxidant effects |
| spellingShingle | Ana C. Gonçalves Radhia Aitfella Lahlou Gilberto Alves Cristina Garcia-Viguera Diego A. Moreno Luís R. Silva Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies Foods bee pollen phenolic characterization biological potential functional food antioxidant effects |
| title | Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies |
| title_full | Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies |
| title_fullStr | Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies |
| title_full_unstemmed | Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies |
| title_short | Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies |
| title_sort | potential activity of abrantes pollen extract biochemical and cellular model studies |
| topic | bee pollen phenolic characterization biological potential functional food antioxidant effects |
| url | https://www.mdpi.com/2304-8158/10/11/2804 |
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