Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing
In cattle, the oviduct plays a fundamental role in the reproductive process. Oviductal functions are controlled by the ovarian sex steroids: estradiol and progesterone. Here, we tested the hypothesis that the exposure to contrasting sex steroid milieus differentially impacts the oviductal transcript...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Genomics Data |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213596017300600 |
| _version_ | 1828885668124688384 |
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| author | Angela Maria Gonella-Diaza Sónia Cristina da Silva Andrade Mariana Sponchiado Guilherme Pugliesi Fernando Silveira Mesquita Veerle Van Hoeck Ricardo de Francisco Strefezzi Gustavo R. Gasparin Luiz L. Coutinho Mario Binelli |
| author_facet | Angela Maria Gonella-Diaza Sónia Cristina da Silva Andrade Mariana Sponchiado Guilherme Pugliesi Fernando Silveira Mesquita Veerle Van Hoeck Ricardo de Francisco Strefezzi Gustavo R. Gasparin Luiz L. Coutinho Mario Binelli |
| author_sort | Angela Maria Gonella-Diaza |
| collection | DOAJ |
| description | In cattle, the oviduct plays a fundamental role in the reproductive process. Oviductal functions are controlled by the ovarian sex steroids: estradiol and progesterone. Here, we tested the hypothesis that the exposure to contrasting sex steroid milieus differentially impacts the oviductal transcriptional profile. We manipulated growth of the pre-ovulatory follicle to obtain cows that ovulated a larger (LF group) or a smaller (SF group) follicle. The LF group presented greater proestrus/estrus concentrations of estradiol and metaestrus concentrations of progesterone (Gonella-Diaza et al. 2015 [1], Mesquita et al. 2014 [2]). Also, the LF group was associated with greater fertility in timed-artificial insemination programs (Pugliesi et al. 2016 [3]). Cows were slaughtered on day 4 of the estrous cycle and total RNA was extracted from ampulla and isthmus fragments and analyzed by RNAseq. The resulting reads were mapped to the bovine genome (Bos taurus UMD 3.1, NCBI). The differential expression analyses revealed that 325 and 367 genes in ampulla and 274 and 316 genes in the isthmus were up-regulated and down-regulated in LF samples, respectively. To validate the RNAseq results, transcript abundance of 23 genes was assessed by qPCR and expression patterns were consistent between the two techniques. A functional enrichment analysis was performed using Database for Annotation, Visualization and Integrated Discovery (DAVID) software. Processes enriched in the LF group included tissue morphology changes (extracellular matrix remodeling), cellular changes (proliferation), and secretion changes (growth factors, ions and metal transporters). An overview of the gene expression data was deposited in the NCBI's Gene Expression Omnibus (GEO) and is accessible through the accession number GSE65681. In conclusion, differences in the peri-ovulatory sex steroid milieu modify the oviductal gene expression profiles. Such differences may be associated with the greater fertility of the LF cows. This dataset is useful for further investigations of the oviductal biology and the impact of sex-steroid on the female reproductive tract. |
| first_indexed | 2024-12-13T11:30:46Z |
| format | Article |
| id | doaj.art-7f448b10503940a99742cca44c72c9d8 |
| institution | Directory Open Access Journal |
| issn | 2213-5960 |
| language | English |
| last_indexed | 2024-12-13T11:30:46Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics Data |
| spelling | doaj.art-7f448b10503940a99742cca44c72c9d82022-12-21T23:47:57ZengElsevierGenomics Data2213-59602017-09-0113C272910.1016/j.gdata.2017.06.004Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencingAngela Maria Gonella-Diaza0Sónia Cristina da Silva Andrade1Mariana Sponchiado2Guilherme Pugliesi3Fernando Silveira Mesquita4Veerle Van Hoeck5Ricardo de Francisco Strefezzi6Gustavo R. Gasparin7Luiz L. Coutinho8Mario Binelli9Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, São Paulo, BrazilDepartamento de Genética e Biologia Evolutiva, IB-Universidade de São Paulo, Cidade Universitária, São Paulo, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, São Paulo, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, São Paulo, BrazilFaculdade de Medicina Veterinária, Universidade Federal do Pampa, Uruguaiana, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, São Paulo, BrazilFaculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, São Paulo, BrazilLaboratório de Biotecnologia Animal, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av Pádua Dias, 11, Piracicaba, SP, BrazilLaboratório de Biotecnologia Animal, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av Pádua Dias, 11, Piracicaba, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, São Paulo, BrazilIn cattle, the oviduct plays a fundamental role in the reproductive process. Oviductal functions are controlled by the ovarian sex steroids: estradiol and progesterone. Here, we tested the hypothesis that the exposure to contrasting sex steroid milieus differentially impacts the oviductal transcriptional profile. We manipulated growth of the pre-ovulatory follicle to obtain cows that ovulated a larger (LF group) or a smaller (SF group) follicle. The LF group presented greater proestrus/estrus concentrations of estradiol and metaestrus concentrations of progesterone (Gonella-Diaza et al. 2015 [1], Mesquita et al. 2014 [2]). Also, the LF group was associated with greater fertility in timed-artificial insemination programs (Pugliesi et al. 2016 [3]). Cows were slaughtered on day 4 of the estrous cycle and total RNA was extracted from ampulla and isthmus fragments and analyzed by RNAseq. The resulting reads were mapped to the bovine genome (Bos taurus UMD 3.1, NCBI). The differential expression analyses revealed that 325 and 367 genes in ampulla and 274 and 316 genes in the isthmus were up-regulated and down-regulated in LF samples, respectively. To validate the RNAseq results, transcript abundance of 23 genes was assessed by qPCR and expression patterns were consistent between the two techniques. A functional enrichment analysis was performed using Database for Annotation, Visualization and Integrated Discovery (DAVID) software. Processes enriched in the LF group included tissue morphology changes (extracellular matrix remodeling), cellular changes (proliferation), and secretion changes (growth factors, ions and metal transporters). An overview of the gene expression data was deposited in the NCBI's Gene Expression Omnibus (GEO) and is accessible through the accession number GSE65681. In conclusion, differences in the peri-ovulatory sex steroid milieu modify the oviductal gene expression profiles. Such differences may be associated with the greater fertility of the LF cows. This dataset is useful for further investigations of the oviductal biology and the impact of sex-steroid on the female reproductive tract.http://www.sciencedirect.com/science/article/pii/S2213596017300600RNAseqBeef cattleAmpullaIsthmusSex steroids |
| spellingShingle | Angela Maria Gonella-Diaza Sónia Cristina da Silva Andrade Mariana Sponchiado Guilherme Pugliesi Fernando Silveira Mesquita Veerle Van Hoeck Ricardo de Francisco Strefezzi Gustavo R. Gasparin Luiz L. Coutinho Mario Binelli Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing Genomics Data RNAseq Beef cattle Ampulla Isthmus Sex steroids |
| title | Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing |
| title_full | Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing |
| title_fullStr | Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing |
| title_full_unstemmed | Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing |
| title_short | Oviductal transcriptional profiling of a bovine fertility model by next-generation sequencing |
| title_sort | oviductal transcriptional profiling of a bovine fertility model by next generation sequencing |
| topic | RNAseq Beef cattle Ampulla Isthmus Sex steroids |
| url | http://www.sciencedirect.com/science/article/pii/S2213596017300600 |
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