Isolation of flavonoids from onion skins and their effects on K562 cell viability
To investigate the anti-proliferative activity of flavonoids from onion skins, extraction by 50% ethanol (v/v), soxhlet polar fractionation, pH gradient separation, thin-layer chromatography, and recrystallization methods were used to isolate and purify flavonoids from dry onion skins. Anti-prolifer...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Pharmacological Society
2016-02-01
|
| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/26419 |
| _version_ | 1819137653329625088 |
|---|---|
| author | Guo-Qing Shi Jing Yang Jiang Liu Sheng-Nan Liu Han-Xue Song Wen-En Zhao Yan-Qi Liu |
| author_facet | Guo-Qing Shi Jing Yang Jiang Liu Sheng-Nan Liu Han-Xue Song Wen-En Zhao Yan-Qi Liu |
| author_sort | Guo-Qing Shi |
| collection | DOAJ |
| description | To investigate the anti-proliferative activity of flavonoids from onion skins, extraction by 50% ethanol (v/v), soxhlet polar fractionation, pH gradient separation, thin-layer chromatography, and recrystallization methods were used to isolate and purify flavonoids from dry onion skins. Anti-proliferative activities of some flavonoids obtained on leukemia K562 cell line were deter-mined by MTT assay. Results showed that flavonoids of onion skins were mainly in form of quercetin, kaempferol, isorhamnetin, apigenin-7-O-?-D-glucopyranoside, quercetin-3-O-?-D-glucopyranoside, kaempferol-7-O-?-D-glucopyranoside and rutin. Quercetin and kaempferol decreased K562 cell viability, and quercetin had stronger effect. However, isorhamnetin and rutin exhibited certain proliferation-promoting effects. It suggests that ortho hydroxyl groups on B ring of onion flavonoids might be the key structural elements of their cytotoxic effects on K562 cells, and hydroxyl groups in position 3 or carbonyl groups in position 4 might be one of the structural effect elements. |
| first_indexed | 2024-12-22T10:54:17Z |
| format | Article |
| id | doaj.art-7f4710d4ce8c484b9ff63157f5683fe0 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-22T10:54:17Z |
| publishDate | 2016-02-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-7f4710d4ce8c484b9ff63157f5683fe02022-12-21T18:28:40ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882016-02-0111S110.3329/bjp.v11iS1.26419Isolation of flavonoids from onion skins and their effects on K562 cell viabilityGuo-Qing Shi0Jing Yang1Jiang Liu2Sheng-Nan Liu3Han-Xue Song4Wen-En Zhao5Yan-Qi Liu6School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002National Engineering Laboratory for Further Processing of Wheat and Corn, Henan University of Technology, Henan, Zhengzhou 450000School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002School of Chemical Engineering and Energy, Zhengzhou University, Henan, Zhengzhou 450052School of Food and Bioengineering, Zhengzhou University of Light Industry, Henan, Zhengzhou 450002To investigate the anti-proliferative activity of flavonoids from onion skins, extraction by 50% ethanol (v/v), soxhlet polar fractionation, pH gradient separation, thin-layer chromatography, and recrystallization methods were used to isolate and purify flavonoids from dry onion skins. Anti-proliferative activities of some flavonoids obtained on leukemia K562 cell line were deter-mined by MTT assay. Results showed that flavonoids of onion skins were mainly in form of quercetin, kaempferol, isorhamnetin, apigenin-7-O-?-D-glucopyranoside, quercetin-3-O-?-D-glucopyranoside, kaempferol-7-O-?-D-glucopyranoside and rutin. Quercetin and kaempferol decreased K562 cell viability, and quercetin had stronger effect. However, isorhamnetin and rutin exhibited certain proliferation-promoting effects. It suggests that ortho hydroxyl groups on B ring of onion flavonoids might be the key structural elements of their cytotoxic effects on K562 cells, and hydroxyl groups in position 3 or carbonyl groups in position 4 might be one of the structural effect elements.https://www.banglajol.info/index.php/BJP/article/view/26419FlavonoidK562 cellOnion skinViability |
| spellingShingle | Guo-Qing Shi Jing Yang Jiang Liu Sheng-Nan Liu Han-Xue Song Wen-En Zhao Yan-Qi Liu Isolation of flavonoids from onion skins and their effects on K562 cell viability Bangladesh Journal of Pharmacology Flavonoid K562 cell Onion skin Viability |
| title | Isolation of flavonoids from onion skins and their effects on K562 cell viability |
| title_full | Isolation of flavonoids from onion skins and their effects on K562 cell viability |
| title_fullStr | Isolation of flavonoids from onion skins and their effects on K562 cell viability |
| title_full_unstemmed | Isolation of flavonoids from onion skins and their effects on K562 cell viability |
| title_short | Isolation of flavonoids from onion skins and their effects on K562 cell viability |
| title_sort | isolation of flavonoids from onion skins and their effects on k562 cell viability |
| topic | Flavonoid K562 cell Onion skin Viability |
| url | https://www.banglajol.info/index.php/BJP/article/view/26419 |
| work_keys_str_mv | AT guoqingshi isolationofflavonoidsfromonionskinsandtheireffectsonk562cellviability AT jingyang isolationofflavonoidsfromonionskinsandtheireffectsonk562cellviability AT jiangliu isolationofflavonoidsfromonionskinsandtheireffectsonk562cellviability AT shengnanliu isolationofflavonoidsfromonionskinsandtheireffectsonk562cellviability AT hanxuesong isolationofflavonoidsfromonionskinsandtheireffectsonk562cellviability AT wenenzhao isolationofflavonoidsfromonionskinsandtheireffectsonk562cellviability AT yanqiliu isolationofflavonoidsfromonionskinsandtheireffectsonk562cellviability |