Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development
Selenium (Se) deficiency has been known for many years to be associated with disease, impaired growth and a variety of other metabolic disorders in mammals. Only recently has the major role that Se-containing proteins, designated selenoproteins, play in many aspects of health and development begun t...
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| Format: | Article |
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MDPI AG
2009-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/14/9/3509/ |
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| author | Dolph L. Hatfield Vadim N. Gladyshev Petra A. Tsuji Min-Hyuk Yoo Bradley A. Carlson |
| author_facet | Dolph L. Hatfield Vadim N. Gladyshev Petra A. Tsuji Min-Hyuk Yoo Bradley A. Carlson |
| author_sort | Dolph L. Hatfield |
| collection | DOAJ |
| description | Selenium (Se) deficiency has been known for many years to be associated with disease, impaired growth and a variety of other metabolic disorders in mammals. Only recently has the major role that Se-containing proteins, designated selenoproteins, play in many aspects of health and development begun to emerge. Se is incorporated into protein by way of the Se-containing amino acid, selenocysteine (Sec). The synthesis of selenoproteins is dependent on Sec tRNA for insertion of Sec, the 21st amino acid in the genetic code, into protein. We have taken advantage of this dependency to modulate the expression of Sec tRNA that in turn modulates the expression of selenoproteins by generating transgenic, conditional knockout, transgenic/standard knockout and transgenic/conditional knockout mouse models, all of which involve the Sec tRNA gene, to elucidate the intracellular roles of this protein class. |
| first_indexed | 2024-12-19T02:46:40Z |
| format | Article |
| id | doaj.art-7f4760f901b14960a43fa89c404ce3f6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T02:46:40Z |
| publishDate | 2009-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7f4760f901b14960a43fa89c404ce3f62022-12-21T20:38:50ZengMDPI AGMolecules1420-30492009-09-011493509352710.3390/molecules14093509Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and DevelopmentDolph L. HatfieldVadim N. GladyshevPetra A. TsujiMin-Hyuk YooBradley A. CarlsonSelenium (Se) deficiency has been known for many years to be associated with disease, impaired growth and a variety of other metabolic disorders in mammals. Only recently has the major role that Se-containing proteins, designated selenoproteins, play in many aspects of health and development begun to emerge. Se is incorporated into protein by way of the Se-containing amino acid, selenocysteine (Sec). The synthesis of selenoproteins is dependent on Sec tRNA for insertion of Sec, the 21st amino acid in the genetic code, into protein. We have taken advantage of this dependency to modulate the expression of Sec tRNA that in turn modulates the expression of selenoproteins by generating transgenic, conditional knockout, transgenic/standard knockout and transgenic/conditional knockout mouse models, all of which involve the Sec tRNA gene, to elucidate the intracellular roles of this protein class.http://www.mdpi.com/1420-3049/14/9/3509/mouse modelsseleniumselenocysteine tRNAselenoproteins |
| spellingShingle | Dolph L. Hatfield Vadim N. Gladyshev Petra A. Tsuji Min-Hyuk Yoo Bradley A. Carlson Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development Molecules mouse models selenium selenocysteine tRNA selenoproteins |
| title | Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development |
| title_full | Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development |
| title_fullStr | Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development |
| title_full_unstemmed | Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development |
| title_short | Mouse Models Targeting Selenocysteine tRNA Expression for Elucidating the Role of Selenoproteins in Health and Development |
| title_sort | mouse models targeting selenocysteine trna expression for elucidating the role of selenoproteins in health and development |
| topic | mouse models selenium selenocysteine tRNA selenoproteins |
| url | http://www.mdpi.com/1420-3049/14/9/3509/ |
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