Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention
A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome. Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophiliz...
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| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/12/4350 |
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| author | Gloria I. Solano-Aguilar Sukla Lakshman Jonathan Shao Celine Chen Ethiopia Beshah Harry D. Dawson Bryan Vinyard Steven G. Schroeder Saebyeol Jang Aleksey Molokin Joseph F. Urban |
| author_facet | Gloria I. Solano-Aguilar Sukla Lakshman Jonathan Shao Celine Chen Ethiopia Beshah Harry D. Dawson Bryan Vinyard Steven G. Schroeder Saebyeol Jang Aleksey Molokin Joseph F. Urban |
| author_sort | Gloria I. Solano-Aguilar |
| collection | DOAJ |
| description | A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome. Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks. Host transcriptome changes in the WBC were evaluated by RNA sequencing. Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function. Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling. Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from <i>Lachnospiraceae</i> and <i>Ruminococcaceae</i> families within the order Clostridiales and <i>Erysipelotrichaceae</i> family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet. These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health. |
| first_indexed | 2024-03-10T03:24:29Z |
| format | Article |
| id | doaj.art-7f4d94afba4141a4808a37948d49c655 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:24:29Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-7f4d94afba4141a4808a37948d49c6552023-11-23T09:56:30ZengMDPI AGNutrients2072-66432021-12-011312435010.3390/nu13124350Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding InterventionGloria I. Solano-Aguilar0Sukla Lakshman1Jonathan Shao2Celine Chen3Ethiopia Beshah4Harry D. Dawson5Bryan Vinyard6Steven G. Schroeder7Saebyeol Jang8Aleksey Molokin9Joseph F. Urban10U.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAStatistics and Bioinformatics Group, Agricultural Research Service, U.S. Department of Agriculture, Northeast Area, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAStatistics and Bioinformatics Group, Agricultural Research Service, U.S. Department of Agriculture, Northeast Area, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Genomics and Improvement Laboratory, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAU.S. Department of Agriculture, Northeast Area, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, MD 20705, USAA study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome. Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks. Host transcriptome changes in the WBC were evaluated by RNA sequencing. Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function. Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling. Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from <i>Lachnospiraceae</i> and <i>Ruminococcaceae</i> families within the order Clostridiales and <i>Erysipelotrichaceae</i> family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet. These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.https://www.mdpi.com/2072-6643/13/12/4350fruit and vegetablemicrobiomeimmune response16S rDNARNA sequencingWBC transcriptome |
| spellingShingle | Gloria I. Solano-Aguilar Sukla Lakshman Jonathan Shao Celine Chen Ethiopia Beshah Harry D. Dawson Bryan Vinyard Steven G. Schroeder Saebyeol Jang Aleksey Molokin Joseph F. Urban Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention Nutrients fruit and vegetable microbiome immune response 16S rDNA RNA sequencing WBC transcriptome |
| title | Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention |
| title_full | Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention |
| title_fullStr | Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention |
| title_full_unstemmed | Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention |
| title_short | Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention |
| title_sort | fruit and vegetable supplemented diet modulates the pig transcriptome and microbiome after a two week feeding intervention |
| topic | fruit and vegetable microbiome immune response 16S rDNA RNA sequencing WBC transcriptome |
| url | https://www.mdpi.com/2072-6643/13/12/4350 |
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