Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> Extract
This study aimed to evaluate the potentials for skin-beneficial effects of the hydroethanolic extract of the aerial parts of ironwort (<i>Sideritis raeseri</i> Boiss. & Heldr. subsp. <i>raeseri</i>) and its fractions (petroleum ether, ethyl acetate, n-butanol and water)....
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2677 |
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| author | Nemanja Krgović Miloš Jovanović Ana Alimpić Aradski Teodora Janković Tatjana Stević Gordana Zdunić Sonja Duletić Laušević Katarina Šavikin |
| author_facet | Nemanja Krgović Miloš Jovanović Ana Alimpić Aradski Teodora Janković Tatjana Stević Gordana Zdunić Sonja Duletić Laušević Katarina Šavikin |
| author_sort | Nemanja Krgović |
| collection | DOAJ |
| description | This study aimed to evaluate the potentials for skin-beneficial effects of the hydroethanolic extract of the aerial parts of ironwort (<i>Sideritis raeseri</i> Boiss. & Heldr. subsp. <i>raeseri</i>) and its fractions (petroleum ether, ethyl acetate, n-butanol and water). For these purposes, chemical analysis and in vitro antioxidant, anti-tyrosinase and antimicrobial assays, as well as determination of sun protection factor (SPF), were performed. The highest total phenolic content and quantity of individual flavonoids, chlorogenic acid and verbascoside were determined in the n-butanol fraction, which is in line with results obtained for antioxidant activity. The greatest antimicrobial activity against <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i> and <i>Pseudomonas aeruginosa</i>, the most common causative agents of the skin infections in humans, was exhibited by the ethyl acetate fraction. The strongest anti-tyrosinase activity was shown by the hydroethanolic extract (52.64% of inhibition at 100 μg/mL). Almost all tested samples showed photoprotective activity with SPF higher than 6 obtained at a low concentration of 0.1 mg/mL, which was more than 15 for the butanol fraction. These findings revealed that the hydroethanolic extract of <i>S. raeseri</i> aerial parts could be a valuable ingredient in the formulation of cosmetic and medicinal products intended to overcome various skin disorders. |
| first_indexed | 2024-03-09T19:33:43Z |
| format | Article |
| id | doaj.art-7f4e2e7f71734abb870576d2bb8c98f3 |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:33:43Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-7f4e2e7f71734abb870576d2bb8c98f32023-11-24T02:03:20ZengMDPI AGPlants2223-77472022-10-011120267710.3390/plants11202677Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> ExtractNemanja Krgović0Miloš Jovanović1Ana Alimpić Aradski2Teodora Janković3Tatjana Stević4Gordana Zdunić5Sonja Duletić Laušević6Katarina Šavikin7Institute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaInstitute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaInstitute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, SerbiaInstitute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaInstitute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaInstitute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaInstitute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, SerbiaInstitute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaThis study aimed to evaluate the potentials for skin-beneficial effects of the hydroethanolic extract of the aerial parts of ironwort (<i>Sideritis raeseri</i> Boiss. & Heldr. subsp. <i>raeseri</i>) and its fractions (petroleum ether, ethyl acetate, n-butanol and water). For these purposes, chemical analysis and in vitro antioxidant, anti-tyrosinase and antimicrobial assays, as well as determination of sun protection factor (SPF), were performed. The highest total phenolic content and quantity of individual flavonoids, chlorogenic acid and verbascoside were determined in the n-butanol fraction, which is in line with results obtained for antioxidant activity. The greatest antimicrobial activity against <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i> and <i>Pseudomonas aeruginosa</i>, the most common causative agents of the skin infections in humans, was exhibited by the ethyl acetate fraction. The strongest anti-tyrosinase activity was shown by the hydroethanolic extract (52.64% of inhibition at 100 μg/mL). Almost all tested samples showed photoprotective activity with SPF higher than 6 obtained at a low concentration of 0.1 mg/mL, which was more than 15 for the butanol fraction. These findings revealed that the hydroethanolic extract of <i>S. raeseri</i> aerial parts could be a valuable ingredient in the formulation of cosmetic and medicinal products intended to overcome various skin disorders.https://www.mdpi.com/2223-7747/11/20/2677ironwortphenolicsantioxidant activityantimicrobial activitytyrosinase inhibitionsun protection factor |
| spellingShingle | Nemanja Krgović Miloš Jovanović Ana Alimpić Aradski Teodora Janković Tatjana Stević Gordana Zdunić Sonja Duletić Laušević Katarina Šavikin Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> Extract Plants ironwort phenolics antioxidant activity antimicrobial activity tyrosinase inhibition sun protection factor |
| title | Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> Extract |
| title_full | Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> Extract |
| title_fullStr | Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> Extract |
| title_full_unstemmed | Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> Extract |
| title_short | Bioassay-Guided Skin-Beneficial Effects of Fractionated <i>Sideritis raeseri</i> subsp. <i>raeseri</i> Extract |
| title_sort | bioassay guided skin beneficial effects of fractionated i sideritis raeseri i subsp i raeseri i extract |
| topic | ironwort phenolics antioxidant activity antimicrobial activity tyrosinase inhibition sun protection factor |
| url | https://www.mdpi.com/2223-7747/11/20/2677 |
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