RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i>
Cytochrome P450 (CYP) is a group of important detoxification enzymes found in insects related to their resistance to insecticides. To elucidate the CYP6 family genes of P450, which are potentially related to imidacloprid resistance in <i>Aphis glycines</i>, the CYP6 cDNA sequences of <...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/15/3/188 |
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| author | Shuangyu Li Hongjia Yang Yixiao Wang Lisi Wei Jiawei Lyu Zhimeng Shan Xinxin Zhang Dong Fan |
| author_facet | Shuangyu Li Hongjia Yang Yixiao Wang Lisi Wei Jiawei Lyu Zhimeng Shan Xinxin Zhang Dong Fan |
| author_sort | Shuangyu Li |
| collection | DOAJ |
| description | Cytochrome P450 (CYP) is a group of important detoxification enzymes found in insects related to their resistance to insecticides. To elucidate the CYP6 family genes of P450, which are potentially related to imidacloprid resistance in <i>Aphis glycines</i>, the CYP6 cDNA sequences of <i>A. glycines</i> were studied. The transcriptome of <i>A. glycines</i> was constructed, and the CYP6 cDNA sequences of <i>A. glycines</i> were screened. Their relative expression levels in response to imidacloprid induction were examined through qRT-PCR, and the CYP6s with higher expression levels were used to study the detoxification of imidacloprid through RNA interference and a bioassay. Twelve CYP6s were obtained from the <i>A. glycines</i> transcriptome. These samples were named by the International P450 Nomenclature Committee and registered in GenBank. After 3, 6, 12, 24 and 48 h of induction with LC50 concentrations of imidacloprid, the relative expression levels of these CYP6s increased; the expression level of <i>CYP6CY7</i> experienced the highest increase, being more than 3-fold higher than that of those of the non-imidacloprid-induced CYP6s. After RNA interference for <i>CYP6CY7</i>, the relative expression level of <i>CYP6CY7</i> significantly decreased after 3, 6 and 12 h, while the corresponding P450 enzyme activity decreased after 12 and 24 h. The mortality of <i>A. glycines</i> due to imidacloprid treatment increased by 14.71% at 24 h. <i>CYP6CY7</i> might detoxify imidacloprid in <i>A. glycines</i>. This study provides a theoretical basis for the further study of the mechanism of action of CYP6s and potential new methods for improving insecticidal efficacy. |
| first_indexed | 2024-04-24T18:10:22Z |
| format | Article |
| id | doaj.art-7f4e778b14e8419e8040401db6a2402a |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-04-24T18:10:22Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-7f4e778b14e8419e8040401db6a2402a2024-03-27T13:47:17ZengMDPI AGInsects2075-44502024-03-0115318810.3390/insects15030188RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i>Shuangyu Li0Hongjia Yang1Yixiao Wang2Lisi Wei3Jiawei Lyu4Zhimeng Shan5Xinxin Zhang6Dong Fan7College of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCytochrome P450 (CYP) is a group of important detoxification enzymes found in insects related to their resistance to insecticides. To elucidate the CYP6 family genes of P450, which are potentially related to imidacloprid resistance in <i>Aphis glycines</i>, the CYP6 cDNA sequences of <i>A. glycines</i> were studied. The transcriptome of <i>A. glycines</i> was constructed, and the CYP6 cDNA sequences of <i>A. glycines</i> were screened. Their relative expression levels in response to imidacloprid induction were examined through qRT-PCR, and the CYP6s with higher expression levels were used to study the detoxification of imidacloprid through RNA interference and a bioassay. Twelve CYP6s were obtained from the <i>A. glycines</i> transcriptome. These samples were named by the International P450 Nomenclature Committee and registered in GenBank. After 3, 6, 12, 24 and 48 h of induction with LC50 concentrations of imidacloprid, the relative expression levels of these CYP6s increased; the expression level of <i>CYP6CY7</i> experienced the highest increase, being more than 3-fold higher than that of those of the non-imidacloprid-induced CYP6s. After RNA interference for <i>CYP6CY7</i>, the relative expression level of <i>CYP6CY7</i> significantly decreased after 3, 6 and 12 h, while the corresponding P450 enzyme activity decreased after 12 and 24 h. The mortality of <i>A. glycines</i> due to imidacloprid treatment increased by 14.71% at 24 h. <i>CYP6CY7</i> might detoxify imidacloprid in <i>A. glycines</i>. This study provides a theoretical basis for the further study of the mechanism of action of CYP6s and potential new methods for improving insecticidal efficacy.https://www.mdpi.com/2075-4450/15/3/188<i>Aphis glycines</i>CYP6detoxificationRNA interferenceinsecticidal efficacy |
| spellingShingle | Shuangyu Li Hongjia Yang Yixiao Wang Lisi Wei Jiawei Lyu Zhimeng Shan Xinxin Zhang Dong Fan RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i> Insects <i>Aphis glycines</i> CYP6 detoxification RNA interference insecticidal efficacy |
| title | RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i> |
| title_full | RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i> |
| title_fullStr | RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i> |
| title_full_unstemmed | RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i> |
| title_short | RNA Interference Reveals the Impacts of <i>CYP6CY7</i> on Imidacloprid Resistance in <i>Aphis glycines</i> |
| title_sort | rna interference reveals the impacts of i cyp6cy7 i on imidacloprid resistance in i aphis glycines i |
| topic | <i>Aphis glycines</i> CYP6 detoxification RNA interference insecticidal efficacy |
| url | https://www.mdpi.com/2075-4450/15/3/188 |
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